PMID- 31583656
OWN - NLM
STAT- MEDLINE
DCOM- 20201211
LR  - 20231213
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2061
DP  - 2020
TI  - Cytological Techniques to Study Cytomixis in Plant Male Meiosis.
PG  - 117-129
LID - 10.1007/978-1-4939-9818-0_9 [doi]
AB  - In this chapter we describe cytological techniques to study cytomixis, a process 
      of nuclear migration between plant cells, in squashed plant male meiocytes of 
      Nicotiana tabacum and Secale cereale. To perform immunostaining or fluorescence 
      in situ hybridization (FISH) on meiotic cells involved in cytomixis common 
      protocols are modified. During preparation of specimens for subsequent 
      cytological analysis, it is necessary not only to make DNA and proteins 
      accessible to DNA probes and antibodies, but also to preserve cell cytoplasm. 
      There are also some important modifications in the protocols applied for 
      meiocytes of different plant species. Here we describe protocols for 
      immunostaining and FISH in rigid tobacco male meiocytes with dense cytoplasm and 
      thick callose wall, that tolerate hard squashing, and in soft rye male meiocytes, 
      that are easily damaged upon squashing, both to study cytomixis.
FAU - Mursalimov, Sergey R
AU  - Mursalimov SR
AD  - Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, 
      Novosibirsk, Russian Federation. mursalimovsr@gmail.com.
FAU - Sidorchuk, Yuriy V
AU  - Sidorchuk YV
AD  - Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, 
      Novosibirsk, Russian Federation.
FAU - Deineko, Elena V
AU  - Deineko EV
AD  - Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, 
      Novosibirsk, Russian Federation.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - *Cytogenetic Analysis/methods
MH  - In Situ Hybridization, Fluorescence
MH  - *Meiosis
MH  - Plant Cells
MH  - Plants/*genetics
MH  - Nicotiana/genetics
OTO - NOTNLM
OT  - Cytomixis
OT  - Immunolocalization
OT  - Meiosis
OT  - Rye
OT  - Tobacco
EDAT- 2019/10/05 06:00
MHDA- 2020/12/15 06:00
CRDT- 2019/10/05 06:00
PHST- 2019/10/05 06:00 [entrez]
PHST- 2019/10/05 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
AID - 10.1007/978-1-4939-9818-0_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2020;2061:117-129. doi: 10.1007/978-1-4939-9818-0_9.