PMID- 3158542
OWN - NLM
STAT- MEDLINE
DCOM- 19850717
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 185
IP  - 1
DP  - 1985 Jun 3
TI  - The identification and characterization of alpha-N-acetylated beta-endorphin in 
      the human pituitary gland.
PG  - 109-11
AB  - By using a radioimmunoassay specific for alpha-N-acetyl beta-endorphin and its 
      C-terminally shortened forms, we have established the presence of immunoreactive 
      alpha-N-acetyl endorphin (irNacEP) in extracts of five postmortem human pituitary 
      glands (2.27 +/- 0.64 ng/gland). This immunoreactivity has been further 
      characterized by subjecting these extracts to reverse-phase high-performance 
      liquid chromatography (RP-HPLC). In all cases the major peaks of irNacEP 
      co-migrated with synthetic human standard alpha-N-acetyl alpha-endorphin (Nac 
      alpha EP), alpha-N-acetyl gamma-endorphin (Nac gamma EP) and Nac beta EP. These 
      studies thus represent the initial demonstration that alpha-N-acetylation of 
      beta-endorphin and its shorter molecular forms occurs in the human pituitary 
      gland.
FAU - Smith, A I
AU  - Smith AI
FAU - Cheng, M C
AU  - Cheng MC
FAU - Funder, J W
AU  - Funder JW
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Endorphins)
RN  - 60617-12-1 (beta-Endorphin)
RN  - 75719-50-5 (N-acetyl-beta-endorphin)
SB  - IM
MH  - Acetylation
MH  - Aged
MH  - Chromatography, High Pressure Liquid
MH  - Endorphins/*analysis/metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pituitary Gland/*analysis/metabolism
MH  - Radioimmunoassay
MH  - beta-Endorphin
EDAT- 1985/06/03 00:00
MHDA- 1985/06/03 00:01
CRDT- 1985/06/03 00:00
PHST- 1985/06/03 00:00 [pubmed]
PHST- 1985/06/03 00:01 [medline]
PHST- 1985/06/03 00:00 [entrez]
AID - 0014-5793(85)80750-X [pii]
AID - 10.1016/0014-5793(85)80750-x [doi]
PST - ppublish
SO  - FEBS Lett. 1985 Jun 3;185(1):109-11. doi: 10.1016/0014-5793(85)80750-x.