PMID- 31585654
OWN - NLM
STAT- MEDLINE
DCOM- 20200128
LR  - 20200128
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 237
DP  - 2019 Oct
TI  - Indirect assessment of porcine reproductive and respiratory syndrome virus status 
      in pigs prior to weaning by sampling sows and the environment.
PG  - 108406
LID - S0378-1135(19)30640-6 [pii]
LID - 10.1016/j.vetmic.2019.108406 [doi]
AB  - There is a need to develop cost effective approaches to sample large populations 
      in particular to determine the disease status of pigs prior to weaning. In this 
      study we assessed the presence of the porcine reproductive and respiratory 
      syndrome virus (PRRSV) in the environment (surfaces and air) of farrowing rooms, 
      and udder skin of lactating sows as an indirect measure of piglet PRRSV status. 
      Samples were collected at processing and weaning every three weeks for 23 weeks 
      after a PRRSV outbreak was diagnosed in a swine breeding herd. PRRSV was detected 
      at processing in udder skin wipes, environmental wipes and airborne deposited 
      particle samples up to 14 weeks post outbreak and at weaning in udder skin wipes 
      up to 17 weeks post outbreak. Similar sensitivities were observed for udder skin 
      wipes (43% [95% CI: 23%-66%]) and surface wipes (57% [95% CI: 34%-77%]) when 
      compared to serum at the litter level from piglets at processing. PRRSV was 
      detected in the environment and the udder skin of lactating sows, which indicates 
      that aggregate samples of the environment or lactating sows may be used to 
      evaluate the PRRSV status of the herd in pigs prior to weaning. However, the use 
      of environmental samples to detect PRRSV by RT-PCR should not be used as the 
      single method to assess the PRRSV status at the litter level. Furthermore, our 
      findings also highlight potential sources of PRRSV infection for piglets in 
      breeding herds.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Vilalta, Carles
AU  - Vilalta C
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave, St Paul, MN 55108, USA. Electronic 
      address: cvilalta@umn.edu.
FAU - Sanhueza, Juan
AU  - Sanhueza J
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave, St Paul, MN 55108, USA.
FAU - Garrido, Jorge
AU  - Garrido J
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave, St Paul, MN 55108, USA.
FAU - Murray, Deb
AU  - Murray D
AD  - New Fashion Pork, Jackson, MN, USA.
FAU - Morrison, Robert
AU  - Morrison R
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave, St Paul, MN 55108, USA.
FAU - Corzo, Cesar A
AU  - Corzo CA
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave, St Paul, MN 55108, USA.
FAU - Torremorell, Montserrat
AU  - Torremorell M
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, 1365 Gortner Ave, St Paul, MN 55108, USA.
LA  - eng
PT  - Journal Article
DEP - 20190903
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Aging
MH  - Animal Husbandry
MH  - Animals
MH  - *Environmental Microbiology
MH  - Female
MH  - *Housing, Animal
MH  - Porcine Reproductive and Respiratory Syndrome/*transmission/virology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Sensitivity and Specificity
MH  - Swine/*virology
MH  - Virus Cultivation
MH  - Weaning
OTO - NOTNLM
OT  - Environment
OT  - Monitoring
OT  - Nurse sow
OT  - PRRSV
OT  - Udder
EDAT- 2019/10/06 06:00
MHDA- 2020/01/29 06:00
CRDT- 2019/10/06 06:00
PHST- 2019/05/30 00:00 [received]
PHST- 2019/09/02 00:00 [revised]
PHST- 2019/09/02 00:00 [accepted]
PHST- 2019/10/06 06:00 [entrez]
PHST- 2019/10/06 06:00 [pubmed]
PHST- 2020/01/29 06:00 [medline]
AID - S0378-1135(19)30640-6 [pii]
AID - 10.1016/j.vetmic.2019.108406 [doi]
PST - ppublish
SO  - Vet Microbiol. 2019 Oct;237:108406. doi: 10.1016/j.vetmic.2019.108406. Epub 2019 
      Sep 3.