PMID- 31586845
OWN - NLM
STAT- MEDLINE
DCOM- 20191227
LR  - 20191227
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 185
DP  - 2019 Dec 15
TI  - How Serratia marcescens HB-4 absorbs cadmium and its implication on 
      phytoremediation.
PG  - 109723
LID - S0147-6513(19)31054-1 [pii]
LID - 10.1016/j.ecoenv.2019.109723 [doi]
AB  - A novel strain Serratia marcescens HB-4 with high Cadmium adsorption capacity was 
      isolated from heavy metal contaminated soil in Hunan province, China. S. 
      marcescens HB-4 reduced the concentration of Cd present in wastewater to less 
      than 0.1 mg/L when the inlet stream contained no higher than 5.0 mg/L Cd. After 
      treatment, wastewater meets Integrated Wastewater Discharge Standard of China 
      (GB8978-1996). The naturally dead S. marcescens HB-4 still maintained over 80% of 
      its Cd adsorption capacity. Scanning electron microscope (SEM), transmission 
      electron microscope (TEM) and energy dispersive spectroscopy (EDS) results 
      suggested that the mechanism of Cd adsorption can be explained as the synergy of 
      extracellular adsorption, periplasm accumulation and intracellular absorption. 
      The size of the accumulated Cd particular is at the nanometer scale, which can be 
      washed out by EDTA without damaging cell integrity. SDS-polyacrylamide gel 
      electrophoresis experiment showed that the heavy metal binding protein 
      (especially Fe binding protein), transporter, amino acid and histidine 
      periplasmic binding proteins and oxidoreductases were responsible for Cd removal. 
      The pot experiment of S. marcescens HB-4 combined with Houttuynia cordata to 
      detoxify Cd contaminated soil showed that the cadmium content in the aboveground 
      and underground parts of Houttuynia cordata increased by 34.48% and 59.13% (w/w), 
      respectively. The cadmium accumulation in Houttuynia cordata increased by 44.27% 
      compared with the blank group which was not combined with S. marcescens HB-4. 
      This work demonstrates that microbial synergistic phytoremediation has a 
      significant potential to treat heavy metal contaminated soil.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Chen, Yakui
AU  - Chen Y
AD  - Key Lab of Industrial Biocatalysis, Ministry of Education, China; Department of 
      Chemical Engineering, Tsinghua University, Beijing, 100084, China.
FAU - Zhu, Qifa
AU  - Zhu Q
AD  - Anhui Southern Tobacco Co., Ltd., Xuancheng, Anhui Province, 242000, China.
FAU - Dong, Xiangzhou
AU  - Dong X
AD  - Anhui Southern Tobacco Co., Ltd., Xuancheng, Anhui Province, 242000, China.
FAU - Huang, Weiwei
AU  - Huang W
AD  - Anhui Southern Tobacco Co., Ltd., Xuancheng, Anhui Province, 242000, China.
FAU - Du, Chenyu
AU  - Du C
AD  - School of Applied Science, The University of Huddersfield, Queensgate, 
      Huddersfield, HD1 3DH, UK. Electronic address: c.du@hud.ac.uk.
FAU - Lu, Diannan
AU  - Lu D
AD  - Key Lab of Industrial Biocatalysis, Ministry of Education, China; Department of 
      Chemical Engineering, Tsinghua University, Beijing, 100084, China. Electronic 
      address: ludiannan@tsinghua.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20191003
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Bacterial Proteins)
RN  - 0 (Chelating Agents)
RN  - 0 (Soil Pollutants)
RN  - 00BH33GNGH (Cadmium)
SB  - IM
MH  - Adsorption
MH  - Bacterial Proteins/metabolism
MH  - Biodegradation, Environmental
MH  - Cadmium/*analysis/metabolism
MH  - Chelating Agents
MH  - China
MH  - Houttuynia/*chemistry
MH  - Protein Binding
MH  - Serratia marcescens/*metabolism
MH  - *Soil Microbiology
MH  - Soil Pollutants/*analysis/metabolism
OTO - NOTNLM
OT  - Binding protein
OT  - Bioremediation
OT  - Extracellular adsorption
OT  - Intracellular absorption
OT  - Microorganism
OT  - Periplasm accumulation
EDAT- 2019/10/07 06:00
MHDA- 2019/12/28 06:00
CRDT- 2019/10/07 06:00
PHST- 2019/08/07 00:00 [received]
PHST- 2019/09/21 00:00 [revised]
PHST- 2019/09/24 00:00 [accepted]
PHST- 2019/10/07 06:00 [pubmed]
PHST- 2019/12/28 06:00 [medline]
PHST- 2019/10/07 06:00 [entrez]
AID - S0147-6513(19)31054-1 [pii]
AID - 10.1016/j.ecoenv.2019.109723 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Dec 15;185:109723. doi: 
      10.1016/j.ecoenv.2019.109723. Epub 2019 Oct 3.