PMID- 31587341
OWN - NLM
STAT- MEDLINE
DCOM- 20210507
LR  - 20210507
IS  - 1440-1681 (Electronic)
IS  - 0305-1870 (Linking)
VI  - 47
IP  - 2
DP  - 2020 Feb
TI  - Stroke-prone spontaneously hypertensive rats have reduced hydroxysteroid 17-beta 
      dehydrogenase 7 levels for low cholesterol biosynthesis.
PG  - 255-262
LID - 10.1111/1440-1681.13188 [doi]
AB  - Reduced serum cholesterol content was recently reported to be one of the factors 
      responsible for cerebral haemorrhage. Stroke-prone spontaneously hypertensive 
      rats (SHRSP) are known to have lower serum cholesterol content than normotensive 
      Wistar-Kyoto rats (WKY). We previously reported that lower levels of mevalonate 
      pyrophosphate decarboxylase (MPD) and squalene epoxidase (SQE), which are 
      associated with cholesterol biosynthesis in the liver, are involved in the low 
      serum cholesterol content in SHRSP. Here, we investigate the levels of sterol 
      14-demethylase (CYP51), methylsterol monooxygenase (SC4MOL), and hydroxysteroid 
      17-beta dehydrogenase 7 (HSD17B7), which contribute to the cholesterol synthesis 
      pathway in the conversion of lanosterol to zymosterol, in SHRSP and WKY. The 
      HSD17B7 mRNA levels in the liver of SHRSP were markedly lower than those in WKY, 
      whereas no significant differences were observed in CYP51 and SC4MOL levels in 
      the two types of rats. The relative levels of protein, heteronuclear RNA, and 
      mRNA of HSD17B7 were also significantly lower in SHRSP than in WKY. The 
      degradation rates of HSD17B7 were the same in SHRSP and WKY. The protein levels 
      of HSD17B7 were not significantly reduced in tissues other than the liver, 
      including the brain, lung, heart, spleen, kidney, and testis, in SHRSP. Moreover, 
      HSD17B7 activity was significantly lower in SHRSP than in WKY. Thus, our results 
      indicated that low protein levels and activity of HSD17B7 are responsible for the 
      reduced cholesterol content in SHRSP, indicating that HSD17B7, along with MPD and 
      SQE, is involved in the decreased cholesterol synthesis in the liver of SHRSP.
CI  - (c) 2019 John Wiley & Sons Australia, Ltd.
FAU - Matsuoka, Hiroshi
AU  - Matsuoka H
AUID- ORCID: 0000-0001-9423-5473
AD  - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, 
      Hiroshima, Japan.
FAU - Uchino, Yuka
AU  - Uchino Y
AD  - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, 
      Hiroshima, Japan.
FAU - Choshi, Mayuko
AU  - Choshi M
AD  - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, 
      Hiroshima, Japan.
FAU - Nakamura, Tetsuya
AU  - Nakamura T
AD  - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, 
      Hiroshima, Japan.
FAU - Michihara, Akihiro
AU  - Michihara A
AD  - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, 
      Hiroshima, Japan.
LA  - eng
PT  - Journal Article
DEP - 20191121
PL  - Australia
TA  - Clin Exp Pharmacol Physiol
JT  - Clinical and experimental pharmacology & physiology
JID - 0425076
RN  - 0 (Hsd17b7 protein, rat)
RN  - 0 (Phosphoproteins)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cholesterol/*biosynthesis
MH  - Hepatocytes/metabolism
MH  - Hypertension/genetics/*metabolism
MH  - Male
MH  - Phosphoproteins/genetics/*metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Rats, Inbred WKY
MH  - Species Specificity
MH  - Stroke/genetics/*metabolism
OTO - NOTNLM
OT  - cholesterol biosynthesis
OT  - hydroxysteroid 17-beta dehydrogenase 7
OT  - liver
OT  - stroke-prone spontaneously hypertensive rats
EDAT- 2019/10/07 06:00
MHDA- 2021/05/08 06:00
CRDT- 2019/10/07 06:00
PHST- 2019/02/13 00:00 [received]
PHST- 2019/09/27 00:00 [revised]
PHST- 2019/09/29 00:00 [accepted]
PHST- 2019/10/07 06:00 [pubmed]
PHST- 2021/05/08 06:00 [medline]
PHST- 2019/10/07 06:00 [entrez]
AID - 10.1111/1440-1681.13188 [doi]
PST - ppublish
SO  - Clin Exp Pharmacol Physiol. 2020 Feb;47(2):255-262. doi: 10.1111/1440-1681.13188. 
      Epub 2019 Nov 21.