PMID- 31589967
OWN - NLM
STAT- MEDLINE
DCOM- 20200323
LR  - 20200323
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 247
DP  - 2020 Jan 30
TI  - Euryops arabicus displays anti-inflammatory activities in experimental models.
PG  - 112278
LID - S0378-8741(19)30783-4 [pii]
LID - 10.1016/j.jep.2019.112278 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Euryops arabicus (Asteraceae) is grown in Arab 
      Peninsula. Its aerial parts possess ethnomedicinal applications against several 
      inflammatory conditions. AIM OF THE STUDY: To evaluate the anti-inflammatory 
      activity of Euryops arabicus (E. arabicus) organic extract as well as its major 
      polymethoxylated flavonoids. MATERIALS AND METHODS: Acute toxicity of the total 
      extract of E. ararbicus was evaluated by assessing LD(50). In vivo 
      anti-inflammatory activity was evaluated in rats injected with carrageenan in the 
      plantar area. Paw edema volume, histological changes and rats'stair climbing and 
      motility were assessed. In vitro anti-inflammatory activity of the isolated 
      compounds was evaluated in peripheral blood mononuclear cells (PBMCs) challenged 
      with carrageenan. Inflammation markers were assessed in cellular lysates and 
      collected media. RESULTS: The extract was found safe and considered unclassified 
      with an oral LD(50) > 2000 mg/kg in rats. Pretreatment of rats with a total 
      extract of E. arabicus at doses of 100 and 250 mg/kg significantly inhibited 
      carrageenan-induced increase in paw edema volume and histopathological changes. 
      Also, it significantly ameliorated diminution of climbing and motility. 
      Phytochemical studies led to the isolation and identification of five 
      polymethoxylated flavonoids. The anti-inflammatory properties of the isolated 
      compounds were evaluated in carrageenan-challenged peripheral blood mononuclear 
      cells (PBMCs). All compounds exhibited appreciable antioxidant activities. 
      Further, pre-incubation of the cells with the isolated metabolites significantly 
      ameliorated the rise in cyclo-oxygenase-2 (COX-2), inducible nitric oxide 
      synthase (iNOS) and monocyte chemoattractant protein-1 (MCP-1) induced by 
      carrageenan challenge. Further, the compounds inhibited the leakage of 
      interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and myeloperoxidase (MPO) in media 
      collected from stimulated cells. CONCLUSION: E. arabicus exhibited in vivo 
      anti-inflammatory effects in the carrageenan model as it ameliorated rat paw 
      edema, histopathological changes and movement dysfunction. in vitro activity of 
      isolated compounds was confirmed in stimulated PBMCs. Thus, the anti-inflammatory 
      activity of E. arabicus can be attributed, at least partly, to its anti-oxidant, 
      anti-inflammatory and anti-chemotactic properties.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Abdel-Lateff, Ahmed
AU  - Abdel-Lateff A
AD  - Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, 
      King Abdulaziz University, Jeddah, PO Box 80260, 21589, Saudi Arabia; Department 
      of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, PO. Box 61511, 
      61519, Egypt. Electronic address: aabdellatteff@kau.edu.sa.
FAU - Alarif, Walied Mohamed
AU  - Alarif WM
AD  - Department of Marine Chemistry, Faculty of Marine Science, King Abdulaziz 
      University, Jeddah, PO. Box 80207, 21589, Saudi Arabia.
FAU - Algandaby, Mardi Mohamed
AU  - Algandaby MM
AD  - Department of Biological Sciences, Faculty of Science, King Abdulaziz University, 
      Jeddah, PO. Box 80203, 21589, Saudi Arabia.
FAU - Alburae, Najla Ali
AU  - Alburae NA
AD  - Department of Biological Sciences, Faculty of Science, King Abdulaziz University, 
      Jeddah, PO. Box 80203, 21589, Saudi Arabia; Biology Department, College of 
      Science, Princess Nourah Bint Abdulrahman University, PO. Box 84428, Riyadh, 
      11671, Saudi Arabia.
FAU - Abdel-Naim, Ashraf B
AU  - Abdel-Naim AB
AD  - Department of Pharmacology, Faculty of Pharmacy, King Abdulaziz University, 
      Jeddah, PO Box 80260, 21589, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20191004
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Flavonoids)
RN  - 0 (Plant Extracts)
RN  - 9000-07-1 (Carrageenan)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology/therapeutic use
MH  - Asteraceae/*chemistry
MH  - Carrageenan/immunology
MH  - Chemotaxis/drug effects
MH  - Disease Models, Animal
MH  - Ethnopharmacology
MH  - Flavonoids/*pharmacology/therapeutic use
MH  - Humans
MH  - Inflammation/*drug therapy/immunology
MH  - Lethal Dose 50
MH  - Leukocytes, Mononuclear
MH  - Male
MH  - Medicine, Arabic/methods
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Rats
MH  - Toxicity Tests, Acute
OTO - NOTNLM
OT  - Asteraceae
OT  - Carrageenan
OT  - Climbing
OT  - Inflammation
OT  - Motility
OT  - PBMCs
OT  - Paw edema
EDAT- 2019/10/08 06:00
MHDA- 2020/03/24 06:00
CRDT- 2019/10/08 06:00
PHST- 2019/02/25 00:00 [received]
PHST- 2019/09/05 00:00 [revised]
PHST- 2019/10/03 00:00 [accepted]
PHST- 2019/10/08 06:00 [pubmed]
PHST- 2020/03/24 06:00 [medline]
PHST- 2019/10/08 06:00 [entrez]
AID - S0378-8741(19)30783-4 [pii]
AID - 10.1016/j.jep.2019.112278 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2020 Jan 30;247:112278. doi: 10.1016/j.jep.2019.112278. Epub 
      2019 Oct 4.