PMID- 31591376
OWN - NLM
STAT- MEDLINE
DCOM- 20200309
LR  - 20200309
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 25
DP  - 2019 Oct 8
TI  - Tumor Necrosis Factor-alpha-Induced Protein 8-like 2 Downregulation Reduces CD4(+) T 
      Lymphocyte Apoptosis in Mice with Thermal Injury.
PG  - 7547-7556
LID - 10.12659/MSM.917229 [doi]
AB  - BACKGROUND Cellular immunity plays a crucial role in sepsis, and lymphocyte 
      apoptosis is a key factor in immune homeostasis. Tumor necrosis factor-alpha 
      (TNF-alpha)-induced protein 8-like 2 (TIPE2) is suggested to play a critical role 
      in maintaining immune homeostasis. This study investigated the role of TIPE2 in 
      CD4(+) T lymphocyte apoptosis based on a mouse model of thermal injury. MATERIAL 
      AND METHODS BALB/c male mice were randomized into 6 groups: sham, burn, burn with 
      siTIPE2, burn with siTIPE2 control, burn with TIPE2, and burn with TIPE2 control 
      groups. Splenic CD4(+) T lymphocytes were collected by use of a magnetic cell 
      sorting system. RESULTS We found that TIPE2 downregulation reduced the CD4(+) T 
      lymphocytes apoptosis in the burn with siTIPE2 group, and the protein expression 
      of P-smad2/P-Smad3 were remarkably downregulated. In the burn with siTIPE2 group, 
      Bcl-2 expression was increased compared with that in the sham group (P<0.05), and 
      Bim expression was reduced (P<0.05). In the burn with TIPE2 group, the 
      mitochondrial membrane potential was markedly reduced (P<0.01), while cytochrome 
      C expression was clearly higher than that in the other groups (P<0.01). 
      Activities of caspase-3, -8, and -9 were notably higher in the burn with TIPE2 
      group relative to those for other groups (P<0.05). CONCLUSIONS Downregulation of 
      TIPE2 in vivo can reduce the apoptosis of CD4(+) T lymphocytes following thermal 
      damage, and activate the TGFss downstream signaling of Smad2/Smad3, upregulating 
      Bim, and downregulating Bcl-2.
FAU - Huang, He
AU  - Huang H
AD  - Department of Critical Care Medicine, The 960th Hospital of the PLA (People's 
      Liberation Army) Joint Logistics Support Force, Jinan, Shandong, China 
      (mainland).
FAU - Cui, Yunliang
AU  - Cui Y
AD  - Department of Critical Care Medicine, The 960th Hospital of the PLA (People's 
      Liberation Army) Joint Logistics Support Force, Jinan, Shandong, China 
      (mainland).
FAU - Tian, Zhaotao
AU  - Tian Z
AD  - Department of Critical Care Medicine, The 960th Hospital of the PLA (People's 
      Liberation Army) Joint Logistics Support Force, Jinan, Shandong, China 
      (mainland).
FAU - Li, Tanshi
AU  - Li T
AD  - Emergency Department, Chinese PLA (People's Liberation Army) General Hospital, 
      Beijing, China (mainland).
FAU - Yao, Yongming
AU  - Yao Y
AD  - Trauma Research Center, First Hospital Affiliated to the Chinese PLA (People's 
      Liberation Army) General Hospital, Beijing, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20191008
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad2 protein, mouse)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad3 protein, mouse)
RN  - 0 (TIPE2 protein, mouse)
RN  - 9007-43-6 (Cytochromes c)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Burns/*metabolism/physiopathology
MH  - CD4-Positive T-Lymphocytes/metabolism
MH  - Cytochromes c/metabolism
MH  - Hot Temperature/adverse effects
MH  - Intracellular Signaling Peptides and Proteins/*metabolism/physiology
MH  - Male
MH  - Membrane Potential, Mitochondrial/physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Animal
MH  - RNA, Small Interfering/genetics
MH  - Sepsis/immunology
MH  - Signal Transduction
MH  - Smad2 Protein/metabolism
MH  - Smad3 Protein/metabolism
PMC - PMC6795105
EDAT- 2019/10/09 06:00
MHDA- 2020/03/10 06:00
PMCR- 2019/10/08
CRDT- 2019/10/09 06:00
PHST- 2019/10/09 06:00 [entrez]
PHST- 2019/10/09 06:00 [pubmed]
PHST- 2020/03/10 06:00 [medline]
PHST- 2019/10/08 00:00 [pmc-release]
AID - 917229 [pii]
AID - 10.12659/MSM.917229 [doi]
PST - epublish
SO  - Med Sci Monit. 2019 Oct 8;25:7547-7556. doi: 10.12659/MSM.917229.