PMID- 31592232
OWN - NLM
STAT- MEDLINE
DCOM- 20200427
LR  - 20200427
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 15
IP  - 10
DP  - 2019
TI  - Extracellular Vesicles (EVs) from Lung Adenocarcinoma Cells Promote Human 
      Umbilical Vein Endothelial Cell (HUVEC) Angiogenesis through Yes 
      Kinase-associated Protein (YAP) Transport.
PG  - 2110-2118
LID - 10.7150/ijbs.31605 [doi]
AB  - Yes kinase-associated protein (YAP) plays an important role in angiogenesis and 
      can promote the occurrence and development of many tumor types. However, whether 
      YAP affects tumor angiogenesis in lung cancer, and its potential mechanism in 
      lung cancer, are unknown. In this study, we explored the role of YAP in the 
      angiogenesis of lung adenocarcinoma, and further illustrated its possible 
      mechanism. The expression levels of YAP and the vascular endothelial marker 
      protein CD31 were examined by immunohistochemistry and immunofluorescence in 
      human lung adenocarcinoma tissues, revealing a possible positive correlation 
      between YAP and CD31 in lung adenocarcinoma. The results of the western blotting 
      (WB) of Human Umbilical Vein Endothelial Cells (HUVECs) after coculture with lung 
      adenocarcinoma H1975 cells, H1975 cell-supernatants and H1975-derived EVs showed 
      that YAP derived from H1975 cells can enter HUVECs via EVs. These results were 
      confirmed by immunofluorescence. Finally, we generated H1975 low-YAP expression 
      cells by transfecting the cells with a shYAP lentivirus, and confirmed that the 
      low expression of YAP in H1975 cells inhibits HUVEC angiogenesis by reducing the 
      amount of YAP that enters HUVECs. We found, for the first time, that YAP promotes 
      angiogenesis in lung adenocarcinoma via EVs, at least partially. Our work may 
      provide a promising method for lung cancer treatment by targeting angiogenesis in 
      the future.
CI  - (c) The author(s).
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Respiration, Nanjing First Hospital, Nanjing Medical University, 68 
      Changle Road, Nanjing, Jiangsu 210006, People' s Republic of China.
FAU - Dong, Liyang
AU  - Dong L
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, 
      438 Jiefang Road, Zhenjiang, Jiangsu 212002, People' s Republic of China.
FAU - Zhong, Hai
AU  - Zhong H
AD  - Department of Respiration, Jiangning Hospital, Nanjing Medical University, 168 
      Gushan Road, Nanjing, Jiangsu 211101, People' s Republic of China.
FAU - Yang, Linfei
AU  - Yang L
AD  - Department of Respiration, Nanjing First Hospital, Nanjing Medical University, 68 
      Changle Road, Nanjing, Jiangsu 210006, People' s Republic of China.
FAU - Li, Qian
AU  - Li Q
AD  - Department of Respiration, Nanjing First Hospital, Nanjing Medical University, 68 
      Changle Road, Nanjing, Jiangsu 210006, People' s Republic of China.
FAU - Su, Chuan
AU  - Su C
AD  - Jiangsu Key Laboratory of Pathogen Biology, Department of Pathogen Biology and 
      Immunology, Center for Global Health, Nanjing Medical University, Nanjing, 
      Jiangsu 211166, People's Republic of China.
FAU - Gu, Wei
AU  - Gu W
AD  - Department of Respiration, Nanjing First Hospital, Nanjing Medical University, 68 
      Changle Road, Nanjing, Jiangsu 210006, People' s Republic of China.
FAU - Qian, Yingying
AU  - Qian Y
AD  - Department of Respiration, Nanjing First Hospital, Nanjing Medical University, 68 
      Changle Road, Nanjing, Jiangsu 210006, People' s Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190807
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Platelet Endothelial Cell Adhesion Molecule-1)
RN  - 0 (Transcription Factors)
RN  - 0 (YY1AP1 protein, human)
SB  - IM
MH  - Adenocarcinoma of Lung/genetics/*metabolism
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Extracellular Vesicles/*metabolism
MH  - Human Umbilical Vein Endothelial Cells/*metabolism
MH  - Humans
MH  - Lung Neoplasms/*metabolism
MH  - Microscopy, Electron, Transmission
MH  - Platelet Endothelial Cell Adhesion Molecule-1/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Transcription Factors/genetics/*metabolism
PMC - PMC6775294
OTO - NOTNLM
OT  - EVs
OT  - H1975 cell
OT  - YAP
OT  - angiogenesis
OT  - lung adenocarcinoma
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2019/10/09 06:00
MHDA- 2020/04/28 06:00
PMCR- 2019/01/01
CRDT- 2019/10/09 06:00
PHST- 2018/11/18 00:00 [received]
PHST- 2019/05/06 00:00 [accepted]
PHST- 2019/10/09 06:00 [entrez]
PHST- 2019/10/09 06:00 [pubmed]
PHST- 2020/04/28 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - ijbsv15p2110 [pii]
AID - 10.7150/ijbs.31605 [doi]
PST - epublish
SO  - Int J Biol Sci. 2019 Aug 7;15(10):2110-2118. doi: 10.7150/ijbs.31605. eCollection 
      2019.