PMID- 31592623
OWN - NLM
STAT- MEDLINE
DCOM- 20210719
LR  - 20210719
IS  - 1543-2742 (Electronic)
IS  - 1526-484X (Linking)
VI  - 29
IP  - 6
DP  - 2019 Nov 1
TI  - The Effect of Carbohydrate Ingestion Following Eccentric Resistance Exercise on 
      AKT/mTOR and ERK Pathways: A Randomized, Double-Blinded, Crossover Study.
PG  - 664-670
LID - ijsnem.2019-0075 [pii]
LID - 10.1123/ijsnem.2019-0075 [doi]
AB  - PURPOSE: To determine the acute effects of carbohydrate (CHO) ingestion following 
      a bout of maximal eccentric resistance exercise on key anabolic kinases of 
      mammalian target of rapamycin and extracellular signal-regulated kinase (ERK) 
      pathways. The authors' hypothesis was that the activation of anabolic signaling 
      pathways known to be upregulated by resistance exercise would be further 
      stimulated by the physiological hyperinsulinemia resulting from CHO 
      supplementation. METHODS: Ten resistance-trained men were randomized in a 
      crossover, double-blind, placebo (PLA)-controlled manner to ingest either a 
      noncaloric PLA or 3 g/kg of CHO beverage throughout recovery from resistance 
      exercise. Muscle biopsies were collected at rest, immediately after a single bout 
      of intense lower body resistance exercise, and after 3 hr of recovery. RESULTS: 
      CHO ingestion elevated plasma glucose and insulin concentrations throughout 
      recovery compared with PLA ingestion. The ERK pathway (phosphorylation of ERK1/2 
      [Thr202/Tyr204], RSK [Ser380], and p70S6K [Thr421/Ser424]) was markedly activated 
      immediately after resistance exercise, without any effect of CHO supplementation. 
      The phosphorylation state of AKT (Thr308) was unchanged postexercise in the PLA 
      trial and increased at 3 hr of recovery above resting with ingestion of CHO 
      compared with PLA. Despite stimulating-marked phosphorylation of AKT, CHO 
      ingestion did not enhance resistance exercise-induced phosphorylation of p70S6K 
      (Thr389) and rpS6 (Ser235/236 and Ser240/244). CONCLUSION: CHO supplementation 
      after resistance exercise and hyperinsulinemia does not influence the ERK pathway 
      nor the mTORC1 target p70S6K and its downstream proteins, despite the increased 
      AKT phosphorylation.
FAU - Figueiredo, Vandre C
AU  - Figueiredo VC
AD  - University of Auckland.
FAU - Farnfield, Michelle M
AU  - Farnfield MM
AD  - Deakin University.
FAU - Ross, Megan L R
AU  - Ross MLR
AD  - Australian Catholic University.
AD  - Australian Institute of Sport.
FAU - Gran, Petra
AU  - Gran P
AD  - Deakin University.
FAU - Halson, Shona L
AU  - Halson SL
AD  - Australian Institute of Sport.
FAU - Peake, Jonathan M
AU  - Peake JM
AD  - Queensland Academy of Sport.
AD  - Queensland University of Technology.
FAU - Cameron-Smith, David
AU  - Cameron-Smith D
AD  - University of Auckland.
AD  - AgResearch.
FAU - Markworth, James F
AU  - Markworth JF
AD  - University of Auckland.
AD  - University of Michigan.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - United States
TA  - Int J Sport Nutr Exerc Metab
JT  - International journal of sport nutrition and exercise metabolism
JID - 100939812
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Insulin)
RN  - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Blood Glucose/metabolism
MH  - Cross-Over Studies
MH  - Dietary Carbohydrates/*pharmacology
MH  - Double-Blind Method
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Humans
MH  - Insulin/blood
MH  - Male
MH  - Mechanistic Target of Rapamycin Complex 1/*metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - *Resistance Training
MH  - Young Adult
OTO - NOTNLM
OT  - clinical trial
OT  - insulin
OT  - muscle hypertrophy
OT  - protein synthesis
OT  - recovery
OT  - skeletal muscle
EDAT- 2019/10/09 06:00
MHDA- 2021/07/20 06:00
CRDT- 2019/10/09 06:00
PHST- 2019/03/11 00:00 [received]
PHST- 2019/05/09 00:00 [revised]
PHST- 2019/06/09 00:00 [accepted]
PHST- 2019/10/09 06:00 [pubmed]
PHST- 2021/07/20 06:00 [medline]
PHST- 2019/10/09 06:00 [entrez]
AID - ijsnem.2019-0075 [pii]
AID - 10.1123/ijsnem.2019-0075 [doi]
PST - ppublish
SO  - Int J Sport Nutr Exerc Metab. 2019 Nov 1;29(6):664-670. doi: 
      10.1123/ijsnem.2019-0075.