PMID- 31593984
OWN - NLM
STAT- MEDLINE
DCOM- 20200127
LR  - 20200127
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 60
IP  - 13
DP  - 2019 Oct 1
TI  - Therapeutic Effect of Curcumin, a Plant Polyphenol Extracted From Curcuma longae, 
      in Fibroblasts From Patients With Graves' Orbitopathy.
PG  - 4129-4140
LID - 10.1167/iovs.19-27376 [doi]
AB  - PURPOSE: We examined the therapeutic effect of nontoxic concentrations of 
      curcumin, a plant polyphenol extracted from Curcuma longae, in primary cultures 
      of orbital fibroblasts from Graves' orbitopathy (GO). METHODS: The effect of 
      curcumin on interleukin (IL)-1beta induced-proinflammatory cytokine production was 
      determined using quantitative real-time PCR, enzyme-linked immunosorbent assay 
      (ELISA), and Western blot analysis. Adipogenic differentiation was identified 
      using Oil-Red O staining, and levels of peroxisome proliferator activator gamma 
      (PPARgamma) and CCAAT-enhancer-binding proteins (C/EBP) alpha/beta were determined by 
      Western blot analyses. Antioxidant activity was measured using an 
      oxidant-sensitive fluorescent probe to detect intracellular reactive oxygen 
      species (ROS) generated in response to hydrogen peroxide (H2O2) and cigarette 
      smoke extract (CSE). RESULTS: Treatment with curcumin resulted in a dose- and 
      time-dependent decrease in IL-1beta-induced synthesis of inflammatory cytokines, 
      including IL-6, IL-8, MCP-1, and ICAM-1 at both mRNA and protein levels. Decrease 
      in lipid droplets and expression of PPARgamma and c/EBPalpha/beta were noted in fibroblasts 
      treated with curcumin during adipose differentiation. CSE- or H2O2-induced ROS 
      synthesis was significantly lower in curcumin-treated fibroblasts in comparison 
      with the control. Curcumin significantly suppressed IL-1beta-induced phosphorylated 
      extracellular signal-regulated kinase, Akt, c-Jun NH(2)-terminal kinase, and 
      nuclear factor kappa-light-chain-enhancer of activated B cells, p65 proteins and 
      stimulated beta-catenin translocation into nucleus during adipogenesis. CONCLUSIONS: 
      Curcumin inhibits proinflammatory cytokine production, ROS synthesis, and 
      adipogenesis in orbital fibroblasts of GO patients in vitro possibly related to 
      multiple proinflammatory signaling molecules and beta-catenin pathway. The results 
      of the study support potential use of the curcumin in the treatment of GO.
FAU - Lee, Jihei Sara
AU  - Lee JS
AD  - Department of Ophthalmology, Severance Hospital, Institute of Vision Research, 
      Yonsei University College of Medicine, Seoul, Korea.
FAU - Kim, Jinjoo
AU  - Kim J
AD  - Department of Ophthalmology, Severance Hospital, Institute of Vision Research, 
      Yonsei University College of Medicine, Seoul, Korea.
FAU - Lee, Eun Jig
AU  - Lee EJ
AD  - Department of Endocrinology, Severance Hospital, Yonsei University College of 
      Medicine, Seoul, Korea.
FAU - Yoon, Jin Sook
AU  - Yoon JS
AD  - Department of Ophthalmology, Severance Hospital, Institute of Vision Research, 
      Yonsei University College of Medicine, Seoul, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Cytokines)
RN  - 0 (Plant Extracts)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Curcumin/*pharmacology
MH  - Cytokines/metabolism
MH  - Fibroblasts/*drug effects/metabolism
MH  - Graves Ophthalmopathy/*drug therapy/metabolism
MH  - Humans
MH  - Oxidative Stress/drug effects
MH  - Plant Extracts/*pharmacology
EDAT- 2019/10/09 06:00
MHDA- 2020/01/28 06:00
CRDT- 2019/10/09 06:00
PHST- 2019/10/09 06:00 [entrez]
PHST- 2019/10/09 06:00 [pubmed]
PHST- 2020/01/28 06:00 [medline]
AID - 2752497 [pii]
AID - 10.1167/iovs.19-27376 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2019 Oct 1;60(13):4129-4140. doi: 
      10.1167/iovs.19-27376.