PMID- 31595117
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 0976-3147 (Print)
IS  - 0976-3155 (Electronic)
IS  - 0976-3155 (Linking)
VI  - 10
IP  - 3
DP  - 2019 Jul
TI  - Altered Expression of Heat Shock Protein-27 and Monocyte Chemoattractant 
      Protein-1 after Acute Spinal Cord Injury: A Pilot Study.
PG  - 452-458
LID - 10.1055/s-0039-1697683 [doi]
AB  - Background  Spinal cord injury (SCI) leads to serious complications involving 
      primary trauma and progressive loss due to inflammation, local ischemia, or 
      infection. Despite a worldwide annual incidence of 15 to 40 cases per million, 
      methylprednisolone is the only treatment available to alleviate neurologic 
      dysfunction; therefore, research is currently focused on identifying novel 
      targets by biochemical and molecular studies. Purpose  Here, we investigated the 
      expression of various molecular markers at the messenger ribonucleic acid (mRNA) 
      and protein level at day 0 and day 30 post-SCI. Methods  Enzyme-linked 
      immunosorbent assay (ELISA) was performed to determine the expression of 
      CASPASE-3 and heat shock protein-27 (HSP-27) in serum samples. Real-time 
      polymerase chain reaction (RT-PCR) was performed to determine the level of mRNA 
      expression of vascular endothelial growth factor receptor-1 (VEGFR-1), VEGFR-2, 
      HSP-27, monocyte chemoattractant protein-1 (MCP-1), and CASPASE-3. Results 
       HSP-27 expression at day 30, as compared with day 0, showed significant 
      downregulation. In contrast, there was elevated expression of MCP-1. ELISA 
      analysis showed no significant change in the expression of CASPASE-3 or HSP-27. 
      Conclusion  There may be possible opposing role of HSP-27 and MCP-1 governing 
      SCI. Their association can be studied by designing in vitro studies.
FAU - Boraiah, Vidyasagar
AU  - Boraiah V
AD  - Department of Orthopaedics, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Modgil, Shweta
AU  - Modgil S
AD  - Department of Neurology, Neuroscience Research Laboratory, Post Graduate 
      Institute of Medical Education and Research, Chandigarh, India.
AD  - Department of Zoology, Panjab University, Chandigarh, India.
FAU - Sharma, Kaushal
AU  - Sharma K
AD  - Department of Neurology, Neuroscience Research Laboratory, Post Graduate 
      Institute of Medical Education and Research, Chandigarh, India.
AD  - Centre for System Biology and Bioinformatics, Panjab University, Chandigarh, 
      India.
FAU - Podder, Vivek
AU  - Podder V
AD  - Department of General Medicine, Kamineni Institute of Medical Sciences, 
      Narketpally, Telangana, India.
FAU - Sivapuram, Madhava Sai
AU  - Sivapuram MS
AD  - Department of General Medicine, Dr. Pinnamaneni Siddhartha Institute of Medical 
      Sciences and Research Foundation, Chinna Avutapalli, Andhra Pradesh, India.
FAU - Miranpuri, Gurwattan S
AU  - Miranpuri GS
AD  - Department of Neurological Surgery, University of Wisconsin School of Medicine 
      and Public Health, Madison, Wisconsin, United States.
FAU - Anand, Akshay
AU  - Anand A
AD  - Department of Neurology, Neuroscience Research Laboratory, Post Graduate 
      Institute of Medical Education and Research, Chandigarh, India.
FAU - Goni, Vijay
AU  - Goni V
AD  - Department of Orthopaedics, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
LA  - eng
PT  - Journal Article
DEP - 20191007
PL  - United States
TA  - J Neurosci Rural Pract
JT  - Journal of neurosciences in rural practice
JID - 101533710
PMC - PMC6779554
OTO - NOTNLM
OT  - CASPASE-3
OT  - angiogenesis
OT  - heat shock protein-27
OT  - inflammation
OT  - molecular markers
OT  - monocyte chemoattractant protein-1
OT  - spinal cord injury
OT  - vascular endothelial growth factor
OT  - vascular endothelial growth factor receptor-1
OT  - vascular endothelial growth factor receptor-2
COIS- Conflict of Interest None declared.
EDAT- 2019/10/09 06:00
MHDA- 2019/10/09 06:01
PMCR- 2019/07/01
CRDT- 2019/10/10 06:00
PHST- 2019/10/10 06:00 [entrez]
PHST- 2019/10/09 06:00 [pubmed]
PHST- 2019/10/09 06:01 [medline]
PHST- 2019/07/01 00:00 [pmc-release]
AID - JNRP3900018 [pii]
AID - 10.1055/s-0039-1697683 [doi]
PST - ppublish
SO  - J Neurosci Rural Pract. 2019 Jul;10(3):452-458. doi: 10.1055/s-0039-1697683. Epub 
      2019 Oct 7.