PMID- 31595252
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 2444-8672 (Electronic)
IS  - 2444-8664 (Print)
IS  - 2444-8664 (Linking)
VI  - 4
IP  - 1
DP  - 2019 Jan-Feb
TI  - Xanthohumol and 8-prenylnaringenin reduce type 2 diabetes-associated oxidative 
      stress by downregulating galectin-3.
PG  - e23
LID - 10.1016/j.pbj.0000000000000023 [doi]
LID - e23
AB  - BACKGROUND: Galectin-3 (Gal3) expression is associated with accumulation of 
      Advanced Glycation End products (AGE), a common feature in diabetes mellitus 
      (DM). The role of Gal3 in oxidative stress is, however, controversial, being 
      considered in the literature to play either a protective role or exacerbating 
      disease. METHODS: Herein, we examined the interplay between Gal3 and oxidative 
      stress in a high-fat diet -induced type 2 DMC57Bl/6 mice model. Because natural 
      polyphenols are known to play antioxidant and anti-inflammatory roles and to 
      modulate metabolic activity, we further evaluated the effect of xanthohumol and 
      8-prenylnaringenin polyphenols in this crosstalk. RESULTS: Gal3 expression was 
      accompanied by 3-nitrotyrosine and AGE production in liver and kidney of diabetic 
      mice compared to healthy animals (fed with standard diet). Oral supplementation 
      with polyphenols decreased the levels of these oxidative biomarkers as evaluated 
      by immunohistochemistry and western blotting. Interestingly, blocking Gal3 by 
      incubating human microvascular endothelial cells with modified citrus pectin 
      increased 3-nitrotyrosine protein expression. CONCLUSIONS: These findings imply 
      that Gal3 overexpression is probably controlling oxidative stress in endothelial 
      cells. In conclusion, our results indicate that supplementation with 
      8-prenylnaringenin or xanthohumol reverses diabetes-associated oxidation in liver 
      and kidney, and consequently decreases this diabetic biomarker that predispose to 
      cardiovascular complications.
CI  - Copyright (c) 2018 The Authors. Published by Wolters Kluwer Health, Inc. on behalf 
      of PBJ-Associacao Porto Biomedical/Porto Biomedical Society. All rights reserved.
FAU - Luis, Carla
AU  - Luis C
AD  - Department of Biomedicine, Unit of Biochemistry, Faculty of Medicine.
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
FAU - Costa, Raquel
AU  - Costa R
AD  - Department of Biomedicine, Unit of Biochemistry, Faculty of Medicine.
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
FAU - Rodrigues, Ilda
AU  - Rodrigues I
AD  - Department of Biomedicine, Unit of Biochemistry, Faculty of Medicine.
FAU - Castela, Angela
AU  - Castela A
AD  - Department of Biomedicine, Unit of Biochemistry, Faculty of Medicine.
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
FAU - Coelho, Pedro
AU  - Coelho P
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
AD  - ESTSP-Escola Superior de Tecnologia da Saude do Porto.
FAU - Guerreiro, Susana
AU  - Guerreiro S
AD  - Department of Biomedicine, Unit of Biochemistry, Faculty of Medicine.
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
FAU - Gomes, Joana
AU  - Gomes J
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
AD  - IPATIMUP-Institute of Molecular Pathology and Immunology, University of Porto.
FAU - Reis, Celso
AU  - Reis C
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
AD  - IPATIMUP-Institute of Molecular Pathology and Immunology, University of Porto.
AD  - Department of Pathology, Faculty of Medicine, University of Porto, Porto, 
      Portugal.
FAU - Soares, Raquel
AU  - Soares R
AD  - Department of Biomedicine, Unit of Biochemistry, Faculty of Medicine.
AD  - i3S, Instituto de Investigacao e Inovacao em Saude, University of Porto.
LA  - eng
PT  - Journal Article
DEP - 20180808
PL  - United States
TA  - Porto Biomed J
JT  - Porto biomedical journal
JID - 101707479
PMC - PMC6750249
OTO - NOTNLM
OT  - 3-nitrotyrosine
OT  - advanced glycation end products
OT  - diet polyphenols
OT  - microvascular endothelial cells
OT  - oxidative stress biomarker
COIS- Sponsorships or competing interests that may be relevant to content are disclosed 
      at the end of this article.The authors report no conflicts of interest.
EDAT- 2018/08/08 00:00
MHDA- 2018/08/08 00:01
PMCR- 2018/08/08
CRDT- 2019/10/10 06:00
PHST- 2018/05/15 00:00 [received]
PHST- 2018/07/11 00:00 [accepted]
PHST- 2019/10/10 06:00 [entrez]
PHST- 2018/08/08 00:00 [pubmed]
PHST- 2018/08/08 00:01 [medline]
PHST- 2018/08/08 00:00 [pmc-release]
AID - PBJ-D-18-00030 [pii]
AID - 10.1016/j.pbj.0000000000000023 [doi]
PST - epublish
SO  - Porto Biomed J. 2018 Aug 8;4(1):e23. doi: 10.1016/j.pbj.0000000000000023. 
      eCollection 2019 Jan-Feb.