PMID- 3159539
OWN - NLM
STAT- MEDLINE
DCOM- 19850812
LR  - 20190919
IS  - 0300-8207 (Print)
IS  - 0300-8207 (Linking)
VI  - 13
IP  - 3
DP  - 1985
TI  - Activation of nuclear chromatin in stretch-dependent growth of tissues.
PG  - 217-25
AB  - The growth of connective tissue cells can be controlled both by mechanical 
      extension and by diffusion gradients. We have used nuclear fluorescence with 
      acridine orange (AO) as a measure of nuclear activation, to study growth control 
      in vivo in rat mesometrium and mouse spine. Frozen sections of spinal muscle 
      taken from animals after two h exercise, revealed nuclear activation in 
      peripheral cells. The number of active muscle nuclei decreased drastically with 
      age. During mesometrial growth in pregnancy, AO fluorescence showed that 
      activated cell nuclei occurred mainly near the capillaries. The width of the zone 
      of activated nuclei was within 20 microns from the capillary walls. AO appears to 
      be a sensitive stain for tracing gradients of growth in intact connective tissue.
FAU - Maroudas, N G
AU  - Maroudas NG
FAU - Wray, S
AU  - Wray S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Chromatin)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange
MH  - Age Factors
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Cell Nucleus/ultrastructure
MH  - Chromatin/*ultrastructure
MH  - Connective Tissue/*ultrastructure
MH  - Female
MH  - Mice
MH  - Physical Exertion
MH  - Pregnancy
MH  - Rats
MH  - Spine/growth & development/ultrastructure
EDAT- 1985/01/01 00:00
MHDA- 1985/01/01 00:01
CRDT- 1985/01/01 00:00
PHST- 1985/01/01 00:00 [pubmed]
PHST- 1985/01/01 00:01 [medline]
PHST- 1985/01/01 00:00 [entrez]
AID - 10.3109/03008208509152401 [doi]
PST - ppublish
SO  - Connect Tissue Res. 1985;13(3):217-25. doi: 10.3109/03008208509152401.