PMID- 31597821
OWN - NLM
STAT- MEDLINE
DCOM- 20200402
LR  - 20200402
IS  - 1881-7823 (Electronic)
IS  - 1881-7815 (Linking)
VI  - 13
IP  - 5
DP  - 2019 Nov 13
TI  - Anti-oxidant, anti-apoptotic, anti-hypoxic and anti-inflammatory conditions 
      induced by PTY-2 against STZ-induced stress in islets.
PG  - 382-393
LID - 10.5582/bst.2019.01181 [doi]
AB  - The earlier assessment of Pueraria tuberosa (PT) has shown anti-diabetic effects 
      through enhancing incretin action and DPP-IV (Dipeptidyl peptidase-IV) 
      inhibition. The aim of this work was to further explore the protective role of 
      aqueous extract of Pueraria tuberosa tuber (PTY-2) against streptozotocin (STZ) 
      induced islet stress in rats. Diabetes was induced by STZ (65 mg/kg body weight) 
      in charles foster male rats. After 60 days of STZ administration, animals with 
      blood glucose levels > 200 g/dL were considered as diabetic. All the rats were 
      later divided into three groups: Group-1 (STZ untreated normal rats), Group-2 
      (Diabetic control), and Group-3 (PTY-2 [50 mg/100 g bw treatment for next 10 days 
      to diabetic rats). The rats were then sacrificed after the 10(th) day of 
      treatment accordingly. STZ treatment led to an increase in expression of Matrix 
      metalloproteinases-9 (MMP-9), Tumour necrosis factor-alpha (Tnf-alpha), Hypoxia inducible 
      factor-alpha (HIF-1alpha), Vascular endothelial growth factor (VEGF), Interleukin-6 
      (IL-6), Protein kinase C-epsilon (PKC-epsilon), Nuclear factor kappa-light-chain-enhancer of 
      activated B-cells (NFkB), and Caspase-3. Reverse Transcriptase-PCR (RT-PCR), 
      Immunohistochemistry and Western-Blot analysis showed an increase in the 
      expressions of Superoxide Dismutase (SOD) and Nephrin, and a decrease in the 
      expressions of NFkB, PKC-epsilon, TNF-alpha, MMP-9, HIF-1alpha, VEGF, Caspase-3 and IL-6 after 
      10 days of PTY-2 treatment. The results showed that PTY-2 favorably changed all 
      the expressions via anti-oxidant, anti-apoptotic, anti-hypoxic and 
      anti-inflammatory pathways, making itself as a protective agent against STZ 
      induced islet stress. Further evaluation of PTY-2 might be helpful in 
      establishing its role in the management of diabetes mellitus.
FAU - Srivastava, Shivani
AU  - Srivastava S
AD  - Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu 
      University, Varanasi, India.
FAU - Pandey, Harsh
AU  - Pandey H
AD  - Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu 
      University, Varanasi, India.
FAU - Singh, Surya Kumar
AU  - Singh SK
AD  - Department of Endocrinology and Metabolism, Institute of Medical Sciences, 
      Banaras Hindu University, Varanasi, India.
FAU - Tripathi, Yamini Bhusan
AU  - Tripathi YB
AD  - Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu 
      University, Varanasi, India.
LA  - eng
PT  - Journal Article
DEP - 20191008
PL  - Japan
TA  - Biosci Trends
JT  - Bioscience trends
JID - 101502754
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Plant Extracts)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Hypoxia/*drug effects
MH  - Diabetes Mellitus, Experimental/drug therapy
MH  - Islets of Langerhans/*drug effects
MH  - Male
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Pueraria/chemistry
MH  - Rats
MH  - Streptozocin/pharmacology
MH  - Stress, Physiological/*drug effects
OTO - NOTNLM
OT  - PTY 2
OT  - STZ
OT  - diabetes
OT  - expressions
OT  - islets
OT  - stress
EDAT- 2019/10/11 06:00
MHDA- 2020/04/03 06:00
CRDT- 2019/10/11 06:00
PHST- 2019/10/11 06:00 [pubmed]
PHST- 2020/04/03 06:00 [medline]
PHST- 2019/10/11 06:00 [entrez]
AID - 10.5582/bst.2019.01181 [doi]
PST - ppublish
SO  - Biosci Trends. 2019 Nov 13;13(5):382-393. doi: 10.5582/bst.2019.01181. Epub 2019 
      Oct 8.