PMID- 31600210
OWN - NLM
STAT- MEDLINE
DCOM- 20200312
LR  - 20200426
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 10
DP  - 2019
TI  - Plasma mitochondrial DNA is elevated in obese type 2 diabetes mellitus patients 
      and correlates positively with insulin resistance.
PG  - e0222278
LID - 10.1371/journal.pone.0222278 [doi]
LID - e0222278
AB  - Cells damaged by mechanical or infectious injury release proinflammatory 
      mitochondrial DNA (mtDNA) fragments into the circulation. We evaluated the 
      relation between plasma levels of mtDNA fragments in obese type 2 diabetes 
      mellitus (T2DM) patients and measures of chronic inflammation and insulin 
      resistance. In 10 obese T2DM patients and 12 healthy control (HC) subjects, we 
      measured levels of plasma cell-free mtDNA with quantitative real-time polymerase 
      chain reaction, and mtDNA damage in skeletal muscle with quantitative alkaline 
      Southern blot. Also, markers of systemic inflammation and oxidative stress in 
      skeletal muscle were measured. Plasma levels of mtDNA fragments, mtDNA damage in 
      skeletal muscle and plasma tumor necrosis factor alpha levels were greater in obese 
      T2DM patients than HC subjects. Also, the abundance of plasma mtDNA fragments in 
      obese T2DM patients levels positively correlated with insulin resistance. To the 
      best of our knowledge, this is the first published evidence that elevated level 
      of plasma mtDNA fragments is associated with mtDNA damage and oxidative stress in 
      skeletal muscle and correlates with insulin resistance in obese T2DM patients. 
      Plasma mtDNA may be a useful biomarker for predicting and monitoring insulin 
      resistance in obese patients.
FAU - Yuzefovych, Larysa V
AU  - Yuzefovych LV
AD  - Department of Pharmacology, College of Medicine, University of South Alabama, 
      Mobile, Alabama, United States of America.
FAU - Pastukh, Viktor M
AU  - Pastukh VM
AD  - Department of Pharmacology, College of Medicine, University of South Alabama, 
      Mobile, Alabama, United States of America.
FAU - Ruchko, Mykhaylo V
AU  - Ruchko MV
AD  - Department of Pharmacology, College of Medicine, University of South Alabama, 
      Mobile, Alabama, United States of America.
FAU - Simmons, Jon D
AU  - Simmons JD
AD  - Department of Pharmacology, College of Medicine, University of South Alabama, 
      Mobile, Alabama, United States of America.
AD  - Department of Surgery, College of Medicine, University of South Alabama, Mobile, 
      Alabama, United States of America.
FAU - Richards, William O
AU  - Richards WO
AD  - Department of Surgery, College of Medicine, University of South Alabama, Mobile, 
      Alabama, United States of America.
FAU - Rachek, Lyudmila I
AU  - Rachek LI
AD  - Department of Pharmacology, College of Medicine, University of South Alabama, 
      Mobile, Alabama, United States of America.
LA  - eng
GR  - K08 GM109113/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191010
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (DNA, Mitochondrial)
SB  - IM
MH  - Biomarkers/blood
MH  - Biopsy
MH  - Blood Glucose/genetics
MH  - DNA, Mitochondrial/*blood
MH  - Diabetes Mellitus, Type 2/*blood/complications/pathology
MH  - Female
MH  - Humans
MH  - Insulin Resistance/*genetics
MH  - Male
MH  - Muscle, Skeletal/metabolism
MH  - Obesity/*blood/complications/pathology
MH  - Oxidative Stress/genetics
PMC - PMC6786592
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/10/11 06:00
MHDA- 2020/03/13 06:00
PMCR- 2019/10/10
CRDT- 2019/10/11 06:00
PHST- 2019/02/19 00:00 [received]
PHST- 2019/08/26 00:00 [accepted]
PHST- 2019/10/11 06:00 [entrez]
PHST- 2019/10/11 06:00 [pubmed]
PHST- 2020/03/13 06:00 [medline]
PHST- 2019/10/10 00:00 [pmc-release]
AID - PONE-D-19-04960 [pii]
AID - 10.1371/journal.pone.0222278 [doi]
PST - epublish
SO  - PLoS One. 2019 Oct 10;14(10):e0222278. doi: 10.1371/journal.pone.0222278. 
      eCollection 2019.