PMID- 31601349
OWN - NLM
STAT- MEDLINE
DCOM- 20191114
LR  - 20220411
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 48
IP  - 4
DP  - 2019 Jul
TI  - [Determination of 5 kinds of fish anesthetics residues in fish by ultra-high 
      performance liquid chromatography-tandem mass spectrometry].
PG  - 640-650
AB  - OBJECTIVE: A method for the simultaneous determination of 5 kinds of fish 
      anesthetics residues in fish has been developed by ultra-high performance liquid 
      chromatography-tandem mass spectrometry(UPLC-MS/MS). Eugenol, methyl-eugenol, 
      methyl-isoeugenol, acetyl-isoeugenol and tricaine methanesulfonate(MS-222) were 
      concerned. METHODS: After homogenization fish samples were extracted by 
      acetonitrile-water(80up harpoon-left 0, V/V), purified by Oasis PRiME HLB solid-phase extraction 
      column. Then after centrifuged and concentrated, the samples were separated by 
      Waters ACQUITY UPLC BEH Phenyl column(2. 1 mmx100 mm, 1. 7 mum). The detection was 
      confirmed and quantified by mass spectrum of triple quadrupole in the multiple 
      reaction monitoring(MRM) mode. RESULTS: The calibration curves showed good 
      linearity in each range with correlation coefficients greater than 0. 995. Three 
      levels spiked recovery experiments were carried out using blank fish mud 
      extraction as substrate, the recoveries ranged from 72. 6% to 106. 0%, the 
      relative standard deviations(RSDs) ranged from 2. 2% to 20. 1%(n=6). The 
      qualitative limits of detections(S/N>3) were 0. 14-0. 30 mug/kg and the 
      quantitative limits(S/N>10) were 0. 5-1. 0 mug/kg. CONSLUSION: The method is 
      simple and easy to operate, with less organic reagent, high sensitivity and good 
      stability. The isomers of methyl eugenol and methyl isoeugenol were successfully 
      separated. It is suitable for the detection of 5 kinds of fish anesthetics in 
      fish.
FAU - Liu, Ping
AU  - Liu P
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Xin, Shuangying
AU  - Xin S
AD  - Market Supervison Administration of Xinji City, Xinji 052360, China.
FAU - Fan, Sai
AU  - Fan S
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Wang, Lili
AU  - Wang L
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Luo, Rencai
AU  - Luo R
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Wu, Guohua
AU  - Wu G
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Zhao, Rong
AU  - Zhao R
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Zhao, Yao
AU  - Zhao Y
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Anesthetics)
SB  - IM
MH  - Anesthetics/chemistry/*metabolism
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - *Fishes
MH  - Seafood
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - UPLC-MS/MS
OT  - eugenol
OT  - fish anesthetic
OT  - food detection
OT  - tricaine methanesulfonate(MS-222)
EDAT- 2019/10/12 06:00
MHDA- 2019/11/15 06:00
CRDT- 2019/10/12 06:00
PHST- 2019/10/12 06:00 [entrez]
PHST- 2019/10/12 06:00 [pubmed]
PHST- 2019/11/15 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2019 Jul;48(4):640-650.