PMID- 31601350
OWN - NLM
STAT- MEDLINE
DCOM- 20191114
LR  - 20200108
IS  - 1000-8020 (Print)
IS  - 1000-8020 (Linking)
VI  - 48
IP  - 4
DP  - 2019 Jul
TI  - [Determination of ochratoxin A and ochratoxin alpha in wine by ultra-performance 
      liquid chromatography tandem mass spectrometry].
PG  - 646-650
AB  - OBJECTIVE: To establish a method for determination of ochratoxin A and ochratoxin 
      alpha in wine by ultra-performance liquid chromatography tandem mass 
      spectrometry(UPLC-MS/MS) based on isotopic internal standard method. METHODS: The 
      wine sample was adjusted to pH 9. 0 by 5% ammonia and concentrated by a MAX solid 
      phase extraction cartridge. The UPLC separation was performed on a ACQUITY BEH 
      C_(18) column(100 mmx2. 1 mm, 1. 7 mum)with a isocratic elution program of 
      acetonitrile and 5 mmol/L ammonium acetate as the mobile phase. Electrospray 
      ionization was applied and operated in the positive ion mode. The concentration 
      of wine was quantified by isotope internal standard. RESULTS: The calibration 
      curves were linear in the concentration range of 1. 0-50. 0 mug/L, the 
      coefficients of correlation were 0. 9996 and 0. 9993, respectively. The limits of 
      detection(LODs) of both were 0. 10 mug/kg, and the limits of quantitative were 0. 
      35 mug/kg. The average recoveries at the spiked levels of 0. 35, 2. 00 and 10. 00 
      mg/kg were 88. 6%-108. 0%, and the relative standard deviations(RSDs) were 2. 
      1%-9. 2%, respectively. CONCLUSION: This method is simple, sensitive, accurate 
      and reliable, which is suitable for the determination of ochratoxin A and 
      ochratoxin alpha in wine.
FAU - Chen, Dong
AU  - Chen D
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Xin, Shuangying
AU  - Xin S
AD  - Market Supervision Administration of Xinji City, Xinji 052360, China.
FAU - Liu, Ping
AU  - Liu P
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Li, Bing
AU  - Li B
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Fan, Sai
AU  - Fan S
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Zhao, Rong
AU  - Zhao R
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
FAU - Wang, Zheng
AU  - Wang Z
AD  - Beijing Center for Disease Control and Prevention, Beijing Center for Preventive 
      Medicine Research, Beijing 100013, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Wei Sheng Yan Jiu
JT  - Wei sheng yan jiu = Journal of hygiene research
JID - 9426367
RN  - 0 (Ochratoxins)
RN  - 16281-39-3 (5-chloro-8-hydroxy-3,4-dihydro-3-methylisocoumarin-7-carboxylic acid)
RN  - 1779SX6LUY (ochratoxin A)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Ochratoxins/*analysis
MH  - *Tandem Mass Spectrometry
MH  - Wine/*analysis
OTO - NOTNLM
OT  - food detection
OT  - isotopic internal standard method
OT  - ochratoxin A
OT  - ochratoxin alpha
OT  - ultra-performance liquid chromatography tandem
OT  - wine
EDAT- 2019/10/12 06:00
MHDA- 2019/11/15 06:00
CRDT- 2019/10/12 06:00
PHST- 2019/10/12 06:00 [entrez]
PHST- 2019/10/12 06:00 [pubmed]
PHST- 2019/11/15 06:00 [medline]
PST - ppublish
SO  - Wei Sheng Yan Jiu. 2019 Jul;48(4):646-650.