PMID- 31601818
OWN - NLM
STAT- MEDLINE
DCOM- 20200124
LR  - 20210110
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 10
IP  - 1
DP  - 2019 Oct 10
TI  - Meiotic crossovers characterized by haplotype-specific chromosome painting in 
      maize.
PG  - 4604
LID - 10.1038/s41467-019-12646-z [doi]
LID - 4604
AB  - Meiotic crossovers (COs) play a critical role in generating genetic variation and 
      maintaining faithful segregation of homologous chromosomes during meiosis. We 
      develop a haplotype-specific fluorescence in situ hybridization (FISH) technique 
      that allows visualization of COs directly on metaphase chromosomes. 
      Oligonucleotides (oligos) specific to chromosome 10 of maize inbreds B73 and 
      Mo17, respectively, are synthesized and labeled as FISH probes. The parental and 
      recombinant chromosome 10 in B73 x Mo17 F(1) hybrids and F(2) progenies can be 
      unambiguously identified by haplotype-specific FISH. Analysis of 58 F(2) plants 
      reveals lack of COs in the entire proximal half of chromosome 10. However, we 
      detect COs located in regions very close to the centromere in recombinant inbred 
      lines from an intermated B73 x Mo17 population, suggesting effective accumulation 
      of COs in recombination-suppressed chromosomal regions through intermating and 
      the potential to generate favorable allelic combinations of genes residing in 
      these regions.
FAU - do Vale Martins, Livia
AU  - do Vale Martins L
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA.
AD  - Department of Horticulture, Michigan State University, East Lansing, MI, 48824, 
      USA.
FAU - Yu, Fan
AU  - Yu F
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA.
AD  - Department of Horticulture, Michigan State University, East Lansing, MI, 48824, 
      USA.
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou, China.
FAU - Zhao, Hainan
AU  - Zhao H
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA.
AD  - Department of Horticulture, Michigan State University, East Lansing, MI, 48824, 
      USA.
FAU - Dennison, Tesia
AU  - Dennison T
AUID- ORCID: 0000-0003-4045-1175
AD  - Genetics and Genomics Graduate Program, Iowa State University, Ames, IA, 50011, 
      USA.
FAU - Lauter, Nick
AU  - Lauter N
AUID- ORCID: 0000-0002-3127-8609
AD  - Genetics and Genomics Graduate Program, Iowa State University, Ames, IA, 50011, 
      USA.
AD  - USDA-ARS Corn Insects and Crop Genetics Research Unit, Iowa State University, 
      Ames, IA, 50011, USA.
FAU - Wang, Haiyan
AU  - Wang H
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA.
AD  - Department of Horticulture, Michigan State University, East Lansing, MI, 48824, 
      USA.
FAU - Deng, Zuhu
AU  - Deng Z
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou, China.
FAU - Thompson, Addie
AU  - Thompson A
AUID- ORCID: 0000-0002-4442-6578
AD  - Department of Plant, Soil and Microbial Sciences, Michigan State University, East 
      Lansing, MI, 48824, USA.
AD  - Michigan State University AgBioResearch, East Lansing, MI, 48824, USA.
FAU - Semrau, Kassandra
AU  - Semrau K
AD  - Arbor Biosciences, Ann Arbor, MI, 48103, USA.
AD  - Department of Natural Sciences, University of Michigan-Dearborn, Dearborn, MI, 
      48128, USA.
FAU - Rouillard, Jean-Marie
AU  - Rouillard JM
AD  - Arbor Biosciences, Ann Arbor, MI, 48103, USA.
AD  - Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, 
      USA.
FAU - Birchler, James A
AU  - Birchler JA
AD  - Division of Biological Sciences, University of Missouri, Columbia, MO, 65211, 
      USA.
FAU - Jiang, Jiming
AU  - Jiang J
AUID- ORCID: 0000-0002-6435-6140
AD  - Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, 
      USA. jiangjm@msu.edu.
AD  - Department of Horticulture, Michigan State University, East Lansing, MI, 48824, 
      USA. jiangjm@msu.edu.
AD  - Michigan State University AgBioResearch, East Lansing, MI, 48824, USA. 
      jiangjm@msu.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20191010
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Oligonucleotides)
SB  - IM
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Plant
MH  - *Crossing Over, Genetic
MH  - Haplotypes/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Meiosis
MH  - Oligonucleotides/genetics
MH  - Reproducibility of Results
MH  - Sequence Analysis, DNA
MH  - Zea mays/*genetics
PMC - PMC6787048
COIS- K.S. and J.M.-R. are employed by Arbor Biosciences. J.M.-R. has personal 
      financial interest in Arbor Biosciences. The remaining authors declare no 
      competing interests.
EDAT- 2019/10/12 06:00
MHDA- 2020/01/25 06:00
PMCR- 2019/10/10
CRDT- 2019/10/12 06:00
PHST- 2019/04/27 00:00 [received]
PHST- 2019/09/20 00:00 [accepted]
PHST- 2019/10/12 06:00 [entrez]
PHST- 2019/10/12 06:00 [pubmed]
PHST- 2020/01/25 06:00 [medline]
PHST- 2019/10/10 00:00 [pmc-release]
AID - 10.1038/s41467-019-12646-z [pii]
AID - 12646 [pii]
AID - 10.1038/s41467-019-12646-z [doi]
PST - epublish
SO  - Nat Commun. 2019 Oct 10;10(1):4604. doi: 10.1038/s41467-019-12646-z.