PMID- 31602210
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 5
DP  - 2019 Nov
TI  - Angelicin inhibits the malignant behaviours of human cervical cancer potentially 
      via inhibiting autophagy.
PG  - 3365-3374
LID - 10.3892/etm.2019.7985 [doi]
AB  - Angelicin is an active compound isolated from the Chinese herb Angelica 
      archangelica, which has been reported to exert antitumor effects by inhibiting 
      malignant behaviors in several types of tumor, including proliferation, colony 
      formation, migration and invasion. However, the effects of angelicin on human 
      cervical cancer cells is yet to be elucidated. The present study evaluated the 
      antitumor effects of angelicin on cervical cancer cells. The results demonstrated 
      that cervical cancer cells were more sensitive to angelicin than cervical 
      epithelial cells. At its IC(30), angelicin inhibited the proliferation of HeLa 
      and SiHa cells by blocking the cell cycle at the G1/G0 phase and inhibiting other 
      malignant behaviors, including colony formation, tumor formation in soft agar, 
      migration and invasion. At the IC(50), angelicin induced cell death potentially 
      by promoting apoptosis. By identifying the hallmarks of autophagy, it was 
      observed that angelicin treatment caused the accumulation of microtubule 
      associated protein 1 light chain 3-beta (LC3B) in the cytoplasm of HeLa and SiHa 
      cells. Western blotting results demonstrated that cleaved LC3B-II and autophagy 
      related proteins (Atg)3, Atg7 and Atg12-5 were upregulated following angelicin 
      treatment. It was also determined that the phosphorylation of mTOR was induced by 
      angelicin treatment. Furthermore, the inhibition of angelicin-induced mTOR 
      phosphorylation did not disrupt its inhibitory effect on autophagy, indicating 
      that angelicin inhibited autophagy in an mTOR-independent manner. Taken together, 
      the present results suggested that angelicin regulated malignant behaviors in 
      cervical cancer cells by inhibiting autophagy in an mTOR-independent manner. 
      Findings suggested that autophagy might be a potential therapeutic target for 
      cervical cancer.
CI  - Copyright: (c) Wang et al.
FAU - Wang, Yiran
AU  - Wang Y
AD  - Department of Obstetrics and Gynecology, Southwest Hospital, Third Military 
      Medical University, Chongqing 400038, P.R. China.
FAU - Chen, Yueqi
AU  - Chen Y
AD  - Department of Orthopedics, Southwest Hospital, Third Military Medical University, 
      Chongqing 400038, P.R. China.
FAU - Chen, Xuedan
AU  - Chen X
AD  - Department of Medical Genetics, College of Basic Medical Science, Third Military 
      Medical University, Chongqing 400038, P.R. China.
FAU - Liang, Yan
AU  - Liang Y
AD  - Department of Respiratory Medicine, The General Hospital of PLA Rocket Force, 
      Beijing 100088, P.R. China.
FAU - Yang, Dapeng
AU  - Yang D
AD  - Department of Obstetrics and Gynecology, Southwest Hospital, Third Military 
      Medical University, Chongqing 400038, P.R. China.
FAU - Dong, Jiao
AU  - Dong J
AD  - Department of Obstetrics and Gynecology, Southwest Hospital, Third Military 
      Medical University, Chongqing 400038, P.R. China.
FAU - Yang, Neng
AU  - Yang N
AD  - Department of Obstetrics and Gynecology, Southwest Hospital, Third Military 
      Medical University, Chongqing 400038, P.R. China.
FAU - Liang, Zhiqing
AU  - Liang Z
AD  - Department of Obstetrics and Gynecology, Southwest Hospital, Third Military 
      Medical University, Chongqing 400038, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190906
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6777310
OTO - NOTNLM
OT  - angelicin
OT  - autophagy
OT  - cervical cancer
OT  - mTOR
OT  - malignant behaviors
EDAT- 2019/10/12 06:00
MHDA- 2019/10/12 06:01
PMCR- 2019/09/06
CRDT- 2019/10/12 06:00
PHST- 2018/09/14 00:00 [received]
PHST- 2019/07/24 00:00 [accepted]
PHST- 2019/10/12 06:00 [entrez]
PHST- 2019/10/12 06:00 [pubmed]
PHST- 2019/10/12 06:01 [medline]
PHST- 2019/09/06 00:00 [pmc-release]
AID - ETM-0-0-7985 [pii]
AID - 10.3892/etm.2019.7985 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Nov;18(5):3365-3374. doi: 10.3892/etm.2019.7985. Epub 2019 Sep 
      6.