PMID- 31602469
OWN - NLM
STAT- MEDLINE
DCOM- 20200204
LR  - 20201209
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 57
IP  - 10
DP  - 2020 Jan 17
TI  - Simple and Rapid Method by Ultra High-Performance Liquid Chromatography (UHPLC) 
      with Ultraviolet Detection for Determination of Efavirenz in Plasma: Application 
      in a Preclinical Pharmacokinetic Study.
PG  - 874-880
LID - 10.1093/chromsci/bmz078 [doi]
AB  - A simple and rapid ultra-high-performance liquid chromatography (UHPLC) method 
      for determination of efavirenz (EFV) in plasma was developed and applied in a 
      preclinical pharmacokinetic study. The method involves only addition of 
      acetonitrile to precipitation of plasma proteins followed by solvent evaporation. 
      The mobile phase consisted of methanol, acetonitrile and 0.1 M formic acid 
      (20:50:30) at a flow rate of 0.3 mL/min with run time of 5 min. A CSH C18 column 
      and a UHPLC-UV system operating at 245 nm were used. There was a linear response 
      in the range of 0.078 to 10 mug/mL, and the equation was obtained by weighting 
      (1/x2) with r2 = 0.9965. The pharmacokinetic disposition of EFV was investigated 
      in rabbits (two groups, n = 7) following a single intravenous administration (IV 
      group) at a dose of 2.7 mg/kg and a single oral administration (oral group) of 
      EFV co-administered with lamivudine (3TC) and tenofovir (TNF) at a dose of 50, 25 
      and 25 mg, respectively. The study demonstrated the applicability of the method 
      for determination of EFV in plasma without interference from other 
      co-administered drugs, and the pharmacokinetic parameters were calculated. The 
      method showed advantages over other methods in the literature, such as simplicity 
      of sample processing and fast results.
CI  - (c) The Author(s) 2019. Published by Oxford University Press. All rights reserved. 
      For permissions, please email: journals.permissions@oup.com.
FAU - Dos Santos Martins, Evelin
AU  - Dos Santos Martins E
AD  - Department of Natural Active Principles and Toxicology, School of Pharmaceutical 
      Sciences, Universidade Estadual Paulista ;;Julio de Mesquita Filho''-UNESP, 
      Araraquara, Sao Paulo, Brazil.
FAU - Oliveira, Jonata Augusto
AU  - Oliveira JA
AD  - Department of Natural Active Principles and Toxicology, School of Pharmaceutical 
      Sciences, Universidade Estadual Paulista ;;Julio de Mesquita Filho''-UNESP, 
      Araraquara, Sao Paulo, Brazil.
FAU - Franchin, Taisa Busaranho
AU  - Franchin TB
AD  - Department of Natural Active Principles and Toxicology, School of Pharmaceutical 
      Sciences, Universidade Estadual Paulista ;;Julio de Mesquita Filho''-UNESP, 
      Araraquara, Sao Paulo, Brazil.
FAU - Silva, Bruna Cristina Ulian
AU  - Silva BCU
AD  - Department of Natural Active Principles and Toxicology, School of Pharmaceutical 
      Sciences, Universidade Estadual Paulista ;;Julio de Mesquita Filho''-UNESP, 
      Araraquara, Sao Paulo, Brazil.
FAU - Candido, Caroline Damico
AU  - Candido CD
AD  - Department of Natural Active Principles and Toxicology, School of Pharmaceutical 
      Sciences, Universidade Estadual Paulista ;;Julio de Mesquita Filho''-UNESP, 
      Araraquara, Sao Paulo, Brazil.
FAU - Peccinini, Rosangela Goncalves
AU  - Peccinini RG
AD  - Department of Natural Active Principles and Toxicology, School of Pharmaceutical 
      Sciences, Universidade Estadual Paulista ;;Julio de Mesquita Filho''-UNESP, 
      Araraquara, Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 0 (Alkynes)
RN  - 0 (Benzoxazines)
RN  - 0 (Cyclopropanes)
RN  - JE6H2O27P8 (efavirenz)
SB  - IM
MH  - Alkynes
MH  - Animals
MH  - Benzoxazines/*blood/chemistry/*pharmacokinetics
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cyclopropanes
MH  - Drug Evaluation, Preclinical
MH  - Limit of Detection
MH  - Linear Models
MH  - Rabbits
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - AIDS
OT  - UHPLC
OT  - bioanalytical method
OT  - efavirenz
OT  - pharmacokinetics
EDAT- 2019/10/12 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/10/12 06:00
PHST- 2018/05/02 00:00 [received]
PHST- 2019/07/15 00:00 [revised]
PHST- 2019/08/27 00:00 [accepted]
PHST- 2019/10/12 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/10/12 06:00 [entrez]
AID - 5584834 [pii]
AID - 10.1093/chromsci/bmz078 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2020 Jan 17;57(10):874-880. doi: 10.1093/chromsci/bmz078.