PMID- 31602543
OWN - NLM
STAT- MEDLINE
DCOM- 20191126
LR  - 20191126
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 164
IP  - 12
DP  - 2019 Dec
TI  - Different in vivo growth of ostreid herpesvirus 1 at 18  degrees C and 22  degrees C alters 
      mortality of Pacific oysters (Crassostrea gigas).
PG  - 3035-3043
LID - 10.1007/s00705-019-04427-2 [doi]
AB  - Seasonally recurrent outbreaks of mass mortality in Pacific oysters (Crassostrea 
      gigas) caused by microvariant genotypes of ostreid herpesvirus 1 (OsHV-1) occur 
      in Europe, New Zealand and Australia. The incubation period for OsHV-1 under 
      experimental conditions is 48-72 hours and depends on water temperature, as does 
      the mortality. An in vivo growth curve for OsHV-1 was determined by quantifying 
      OsHV-1 DNA at 10 time points between 2 and 72 hours after exposure to OsHV-1. The 
      peak replication rate was the same at 18  degrees C and 22  degrees C; however, there was a 
      longer period of amplification leading to a higher peak concentration at 22  degrees C 
      (2.34 x 10(7) copies/mg at 18 hours) compared to 18  degrees C (1.38 x 10(5) copies/mg at 
      12 hours). The peak viral concentration preceded mortality by 72 hours and 
      20 hours at 18  degrees C and 22  degrees C, respectively. Cumulative mortality to day 14 was 
      45.9% at 22  degrees C compared to 0.3% at 18  degrees C. The prevalence of OsHV-1 infection 
      after 14 days at 18  degrees C was 33.3%. No mortality from OsHV-1 occurred when the 
      water temperature in tanks of oysters challenged at 18  degrees C was increased to 22  degrees C 
      for 14 days. The influence of water temperature prior to exposure to OsHV-1 and 
      during the initial virus replication is an important determinant of the outcome 
      of infection in C. gigas.
FAU - de Kantzow, Maximilian C
AU  - de Kantzow MC
AD  - Sydney School of Veterinary Science, Farm Animal Health, University of Sydney, 
      Camden, 2570, Australia.
FAU - Whittington, Richard J
AU  - Whittington RJ
AD  - Sydney School of Veterinary Science, Farm Animal Health, University of Sydney, 
      Camden, 2570, Australia.
FAU - Hick, Paul M
AU  - Hick PM
AUID- ORCID: 0000-0002-6821-2232
AD  - Sydney School of Veterinary Science, Farm Animal Health, University of Sydney, 
      Camden, 2570, Australia. Paul.hick@sydney.edu.au.
LA  - eng
GR  - FRDC: 2014-040/Fisheries Research and Development Corperation/
PT  - Comparative Study
PT  - Journal Article
DEP - 20191010
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Crassostrea/growth & development/*physiology/*virology
MH  - DNA Viruses/genetics/*growth & development
MH  - DNA, Viral/genetics
MH  - Shellfish/*virology
MH  - Temperature
EDAT- 2019/10/12 06:00
MHDA- 2019/11/27 06:00
CRDT- 2019/10/12 06:00
PHST- 2019/05/03 00:00 [received]
PHST- 2019/09/09 00:00 [accepted]
PHST- 2019/10/12 06:00 [pubmed]
PHST- 2019/11/27 06:00 [medline]
PHST- 2019/10/12 06:00 [entrez]
AID - 10.1007/s00705-019-04427-2 [pii]
AID - 10.1007/s00705-019-04427-2 [doi]
PST - ppublish
SO  - Arch Virol. 2019 Dec;164(12):3035-3043. doi: 10.1007/s00705-019-04427-2. Epub 
      2019 Oct 10.