PMID- 31604159
OWN - NLM
STAT- MEDLINE
DCOM- 20200117
LR  - 20200117
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 186
DP  - 2019 Dec 30
TI  - Responses of hemocyanin and energy metabolism to acute nitrite stress in 
      juveniles of the shrimp Litopenaeus vannamei.
PG  - 109753
LID - S0147-6513(19)31084-X [pii]
LID - 10.1016/j.ecoenv.2019.109753 [doi]
AB  - Nitrite is a common toxic substance in culture systems of Litopenaeus vannamei, 
      and the stress may disturb hemocyanin synthesis and energy metabolism and result 
      in shrimp death. In the present study, nitrite at concentrations of 0 (control), 
      3.3 (46.2 NO(2)-N mg/L), 6.6 (92.4) and 9.9 mM (138.6) was used to evaluate the 
      responses of hemocyanin level and energy metabolism in L. vannamei 
      (5.80 +/- 0.44 cm, 1.88 +/- 0.38 g) for 96 h. The mortality rate at 96 h increased 
      with nitrite concentration (50% at 9.9 mM, 40% at 6.6 mM, 30% at 3.3 mM, and 10% 
      at 0 mM). In general, HIF-1alpha and hemocyanin mRNA expression in the nitrite stress 
      groups was upregulated from 6 to 12 h and downregulated from 24 to 96 h. In the 
      hemolymph, nitrite levels were significantly elevated in a dose-dependent manner, 
      and exposure to nitrite stress significantly decreased the oxyhemocyanin content 
      from 24 to 96 h. The glucose and lactate levels in the hemolymph in the nitrite 
      stress groups were higher than those in the control group from 12 to 96 h. 
      Compared with the control group, the shrimp in the nitrite stress groups 
      exhibited decreased glycogen concentrations in the hepatopancreas. The 
      triglyceride (TG) levels in the nitrite stress groups were all higher than those 
      in the control group from 48 to 96 h. The hexokinase (HK) activity in the 
      hepatopancreas and muscle increased in the nitrite stress groups from 48 to 96 h. 
      In general, nitrite stress enhanced the activities of pyruvate kinase (PK), 
      phosphofructokinase (PFK) and lactate dehydrogenase (LDH) in muscle from 24 to 
      96 h. In addition, nitrite stress decreased the activities of succinate 
      dehydrogenase (SDH) and fatty acid synthase (FAS) from 24 to 96 h in the 
      hepatopancreas and muscle. This study indicates that exposure to nitrite stress 
      can enhance the accumulation of nitrite in the hemolymph and then reduce 
      oxygenation and hemocyanin synthesis, leading to tissue hypoxia and thereby 
      resulting in accelerated anaerobic metabolism and the inhibition of aerobic 
      metabolism. The effects of nitrite stress on hemocyanin synthesis and energy 
      metabolism may be one of the reasons for the mortality of L. vannamei in culture 
      systems.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Li, Z S
AU  - Li ZS
AD  - The Key Laboratory of Mariculture (Ocean University of China), Ministry of 
      Education, Qingdao, 266003, China. Electronic address: 844078171@qq.com.
FAU - Ma, S
AU  - Ma S
AD  - The Key Laboratory of Mariculture (Ocean University of China), Ministry of 
      Education, Qingdao, 266003, China.
FAU - Shan, H W
AU  - Shan HW
AD  - The Key Laboratory of Mariculture (Ocean University of China), Ministry of 
      Education, Qingdao, 266003, China. Electronic address: shanhongwei@ouc.edu.cn.
FAU - Wang, T
AU  - Wang T
AD  - The Key Laboratory of Mariculture (Ocean University of China), Ministry of 
      Education, Qingdao, 266003, China.
FAU - Xiao, W
AU  - Xiao W
AD  - The Key Laboratory of Mariculture (Ocean University of China), Ministry of 
      Education, Qingdao, 266003, China.
LA  - eng
PT  - Journal Article
DEP - 20191008
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nitrites)
RN  - 0 (Triglycerides)
RN  - 0 (oxy-beta-hemocyanin)
RN  - 9005-79-2 (Glycogen)
RN  - 9013-72-3 (Hemocyanins)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Anaerobiosis/*drug effects
MH  - Animals
MH  - Aquaculture
MH  - Energy Metabolism/*drug effects
MH  - Glycogen/metabolism
MH  - Hemocyanins/*metabolism
MH  - Hemolymph/drug effects/metabolism
MH  - Hepatopancreas/drug effects/metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Lipid Metabolism/drug effects
MH  - Muscles/drug effects/metabolism
MH  - Nitrites/*toxicity
MH  - Oxygen/*metabolism
MH  - Penaeidae/*drug effects/growth & development/metabolism
MH  - Seafood
MH  - *Stress, Physiological
MH  - Triglycerides/metabolism
OTO - NOTNLM
OT  - Energy metabolism
OT  - Hemocyanin
OT  - Litopenaeus vannamei
OT  - Nitrite stress
OT  - Tissue hypoxia
EDAT- 2019/10/12 06:00
MHDA- 2020/01/18 06:00
CRDT- 2019/10/12 06:00
PHST- 2019/03/01 00:00 [received]
PHST- 2019/05/21 00:00 [revised]
PHST- 2019/10/01 00:00 [accepted]
PHST- 2019/10/12 06:00 [pubmed]
PHST- 2020/01/18 06:00 [medline]
PHST- 2019/10/12 06:00 [entrez]
AID - S0147-6513(19)31084-X [pii]
AID - 10.1016/j.ecoenv.2019.109753 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Dec 30;186:109753. doi: 
      10.1016/j.ecoenv.2019.109753. Epub 2019 Oct 8.