PMID- 31605570 OWN - NLM STAT- MEDLINE DCOM- 20211005 LR - 20211005 IS - 1552-4981 (Electronic) IS - 1552-4973 (Print) IS - 1552-4973 (Linking) VI - 108 IP - 4 DP - 2020 May TI - Orthogonal test design for the optimization of superparamagnetic chitosan plasmid gelatin microspheres that promote vascularization of artificial bone. PG - 1439-1449 LID - 10.1002/jbm.b.34491 [doi] AB - The optimal conditions for the preparation of superparamagnetic chitosan plasmid (pReceiver-M29-VEGF165/DH5a) gelatin microspheres (SPCPGMs) were determined. Then, the performance of the SPCPGMs during neovascularization was evaluated in vivo. The SPCPGMs were prepared through a cross-linking curing method and then filled into the hollow scaffold of an artificial bone. Neovascularization at the bone defect position was histologically examined in samples collected 2, 4, 6, and 8 weeks after the operation. The cellular magnetofection rate of superparamagnetic chitosan nanoparticles/plasmid (pReceiver-M29-VEGF165/DH5a) complexes reached 1-3% under static magnetic field (SMF). Meanwhile, the optimal conditions for SPCPGM fabrication were 20% Fe(3) O(4) (w/v), 4 mg of plasmid, 5.3 mg of glutaraldehyde, and 500 rpm of emulsification rotate speed. Under oscillating magnetic fields (OMFs), 4-6 mug of plasmids was released daily for 21 days. Under the combined application of SMF and OMF, evident neovascularization occurred at the bone defect position 6 weeks after the operation. This result is expected to provide a new type of angiogenesis strategy for the research of bone tissue engineering. CI - (c) 2019 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc. FAU - Tao, Chen AU - Tao C AUID- ORCID: 0000-0002-8352-8892 AD - Department of Orthopaedics, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R China, Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of Stem Cell Therapy. FAU - Lina, Xie AU - Lina X AD - Department of Orthopaedics, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R China, Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of Stem Cell Therapy. FAU - Changxuan, Wang AU - Changxuan W AD - Department of Orthopaedics, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R China, Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of Stem Cell Therapy. FAU - Cong, Luo AU - Cong L AD - Department of Orthopaedics, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R China, Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of Stem Cell Therapy. FAU - Xiaolan, Yang AU - Xiaolan Y AD - Department of Pharmacology, Chongqing Medical University, Yuzhong District, Yixueyuan Road1#, Chongqing, 400016, China. FAU - Tao, Huang AU - Tao H AD - Department of Orthopaedics, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders (Chongqing), China International Science and Technology Cooperation base of Child development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, P.R China, Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of Stem Cell Therapy. FAU - Hong, An AU - Hong A AD - Department of Orthopaedics, The First Affiliated Hospital of Chongqing Medical University, Yuzhong District, Youyi Road 1#, Chongqing, 400016, China. LA - eng PT - Journal Article DEP - 20191012 PL - United States TA - J Biomed Mater Res B Appl Biomater JT - Journal of biomedical materials research. Part B, Applied biomaterials JID - 101234238 RN - 0 (VEGFA protein, human) RN - 0 (Vascular Endothelial Growth Factor A) RN - 9000-70-8 (Gelatin) RN - 9012-76-4 (Chitosan) SB - IM MH - Animals MH - *Chitosan/chemistry/pharmacokinetics/pharmacology MH - *Gelatin/chemistry/pharmacokinetics/pharmacology MH - Gene Transfer Techniques MH - Magnetic Iron Oxide Nanoparticles/*chemistry MH - *Microspheres MH - *Neovascularization, Physiologic/drug effects/genetics MH - *Plasmids/chemistry/genetics/pharmacokinetics/pharmacology MH - Rabbits MH - Radius/*blood supply/metabolism MH - *Vascular Endothelial Growth Factor A/biosynthesis/genetics PMC - PMC7187448 OTO - NOTNLM OT - VEGF OT - angiogenesis OT - magnetic field OT - magnetic gene-loaded microspheres EDAT- 2019/10/13 06:00 MHDA- 2021/10/06 06:00 PMCR- 2020/04/28 CRDT- 2019/10/13 06:00 PHST- 2019/06/13 00:00 [received] PHST- 2019/08/06 00:00 [revised] PHST- 2019/09/02 00:00 [accepted] PHST- 2019/10/13 06:00 [pubmed] PHST- 2021/10/06 06:00 [medline] PHST- 2019/10/13 06:00 [entrez] PHST- 2020/04/28 00:00 [pmc-release] AID - JBMB34491 [pii] AID - 10.1002/jbm.b.34491 [doi] PST - ppublish SO - J Biomed Mater Res B Appl Biomater. 2020 May;108(4):1439-1449. doi: 10.1002/jbm.b.34491. Epub 2019 Oct 12.