PMID- 31605918
OWN - NLM
STAT- MEDLINE
DCOM- 20200107
LR  - 20200107
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 109
DP  - 2020 Jan
TI  - Proliferation and odontogenic differentiation of human umbilical cord mesenchymal 
      stem cells and human dental pulp cells co-cultured in hydrogel.
PG  - 104582
LID - S0003-9969(19)30516-3 [pii]
LID - 10.1016/j.archoralbio.2019.104582 [doi]
AB  - OBJECTIVE: The aim of this study was to evaluate the proliferation and 
      odontogenic differentiation of human dental pulp cells (hDPCs) and human 
      umbilical cord mesenchymal stem cells (hUCMSCs) in three-dimensional co-culture 
      system which was established with the help of bone morphogenetic protein-2 
      (BMP-2) and hydrogel. METHODS: hDPCs and hUCMSCs were cultured in different 
      concentrations of hydrogel to explore the more suitable concentrations for 
      subsequent experiments. hUCMSCs and hDPCs induced by BMP-2 were co-cultured in 
      the hydrogel. MTT assay was used to measure the cell viability. The 
      differentiation into odontoblast-like cells were measured by the mRNA expression 
      of dentin salivary phosphoprotein (DSPP), dentin matrix protein-1 (DMP-1), 
      alkaline phosphatase and osteocalcin. Alizarin red staining was performed for the 
      formation of mineralized nodules. RESULTS: hUCMSCs and hDPCs could grow and 
      proliferate in hydrogel scaffold. The growth rate of cells in lower 
      concentrations hydrogels were higher than that of high concentrations hydrogels 
      (P < 0.05). The study showed that 0.25% hydrogel scaffold was more suitable for 
      subsequent experiments than other groups. Compared with hUCMSCs-monoculture and 
      hDPCs-monoculture, the co-culture groups exhibited more proliferative potential, 
      alkaline phosphatase activity and mineralization nodule formation (P < 0.05). The 
      mRNA expression in co-culture groups were higher than that of 
      hUCMSCs-monoculture, closed to or even higher than that of hDPCs-monoculture. 
      CONCLUSION: 0.25% hydrogel was the suitable concentration in co-culture system 
      for subsequent experiments. The co-culture groups had stronger abilities of 
      odontoblastic differentiation and mineralization than cells-monoculture groups, 
      indicated that the co-culture conditions could regulate cell proliferation and 
      differentiation within a certain range.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Huang, Chunyang
AU  - Huang C
AD  - Guangxi Medical University, No. 22 Shuangyong Road, Nanning, 530021, China.
FAU - Bao, Lirong
AU  - Bao L
AD  - Guangxi Medical University, No. 22 Shuangyong Road, Nanning, 530021, China.
FAU - Lin, Tian
AU  - Lin T
AD  - Guangxi Medical University, No. 22 Shuangyong Road, Nanning, 530021, China.
FAU - Lu, Yanling
AU  - Lu Y
AD  - Guangxi Medical University, No. 22 Shuangyong Road, Nanning, 530021, China.
FAU - Wu, Yu
AU  - Wu Y
AD  - Guangxi Medical University, No. 22 Shuangyong Road, Nanning, 530021, China; 
      College of Stomatology, Guangxi Medical University, No. 10 Shuangyong Road, 
      Nanning, 530021, China. Electronic address: wuyu0912@163.com.
LA  - eng
PT  - Journal Article
DEP - 20191004
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (BGLAP protein, human)
RN  - 0 (DMP1 protein, human)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Hydrogels)
RN  - 0 (Phosphoproteins)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (dentin sialophosphoprotein)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/genetics
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dental Pulp/*cytology
MH  - Extracellular Matrix Proteins/genetics
MH  - Humans
MH  - *Hydrogels
MH  - Mesenchymal Stem Cells/*cytology
MH  - Odontoblasts/cytology
MH  - Osteocalcin/genetics
MH  - Phosphoproteins/genetics
MH  - Sialoglycoproteins/genetics
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - Human dental pulp cells
OT  - Human umbilical cord mesenchymal stem cells
OT  - Hydrogel scaffold
OT  - Three-dimensional culture
EDAT- 2019/10/13 06:00
MHDA- 2020/01/08 06:00
CRDT- 2019/10/13 06:00
PHST- 2019/05/29 00:00 [received]
PHST- 2019/09/26 00:00 [revised]
PHST- 2019/10/01 00:00 [accepted]
PHST- 2019/10/13 06:00 [pubmed]
PHST- 2020/01/08 06:00 [medline]
PHST- 2019/10/13 06:00 [entrez]
AID - S0003-9969(19)30516-3 [pii]
AID - 10.1016/j.archoralbio.2019.104582 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2020 Jan;109:104582. doi: 10.1016/j.archoralbio.2019.104582. Epub 
      2019 Oct 4.