PMID- 31607001
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20200108
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 54
IP  - 10
DP  - 2019 Oct 9
TI  - [MicroRNA-26a-5p targets Wnt5a to regulate osteogenic differentiation of human 
      periodontal ligament stem cell from inflammatory microenvironment].
PG  - 662-669
LID - 10.3760/cma.j.issn.1002-0098.2019.10.003 [doi]
AB  - Objective: To investigate the effect of microRNA-26a-5p on osteogenic 
      differentiation of human periodontal ligament stem cells (hPDLSC) and its related 
      mechanisms. Methods: hPDLSC in periodontal tissues from healthy adults and hPDLSC 
      from periodontitis patients (PPDLSC) were isolated and cultured in vitro, 
      respectively. The PPDLSC were divided into Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ groups. Group Ⅰ is 
      control group, and the other four groups were transiently transfected with 
      miR-NC, miR-26a-5p, antimiR-NC and antimiR-26a-5p lentiviral vectors, 
      respectively. The osteogenic differentiation abilities of the cells in vitro were 
      determined by alizarin red staining, alkaline phosphatase (ALP) activity assay 
      and real-time quantitative PCR (qPCR). Totally 40 male mice (6-weeks) were 
      equally divided into five groups with 8 mice in each group. The PPDLSCs cells 
      (1x10(7)/ml) in Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ groups, which adhered to 
      hydroxyapatine-tricalcium phosphate (HA-TCP), were implanted into the nude mice 
      subcutaneously and the animal models were constructed to analyze the effect of 
      miR-26a-5p on the osteogenic differentiation of PPDLSCs in vivo. PPDLSCs were 
      divided into A, B, C, D groups, and transfected with miR-26a-5p+Wnt5a-Wt, 
      miR-NC+Wnt5a-Wt, miR-26a-5p+Wnt5a-Mut and miR-NC+Wnt5a-Mut in each of the above 
      mentioned 5 groups, respectively. The luciferase activity assay was used to 
      detect the relative luciferase in A, B, C and D groups to analyze the targeting 
      relationship between miR-26a-5p and Wnt5a. Osteogenic differentiation related 
      proteins expression were analyzed by western blotting. Results: hPDLSC and PPDLSC 
      were observed consistent with the characteristics of mesenchymal stem cells and 
      had osteogenic differentiation ability in vitro. Compared with hPDLSC 
      [(89.87+/-8.12)%], the osteogenic capacity of PPDLSC [(31.46+/-6.56)%] was 
      significantly lower (P<0.05). The ALP activity (1.88+/-0.59), calcified nodules 
      (79.88+/-5.92), the expression of the osteogenic differentiation markers 
      Runt-related transcription factor 2 (Runx2) (2.40+/-0.70), ALP (2.10+/-0.60) and 
      osteocalcin (3.00+/-0.90) mRNA in the PPDLSC from Group Ⅲ were significantly higher 
      in comparison with the control group [(0.88+/-0.34), (29.69+/-2.65), (1.30+/-0.30), 
      (0.09+/-0.25), (1.71+/-0.50)], while those from Group Ⅴ[(0.44+/-0.07), (14.83+/-3.05), 
      (0.50+/-0.11), (0.30+/-0.08) and (0.80+/-0.17)] were significantly lower (P<0.05). In 
      vivo studies in nude mice showed that the proportion of the osteogenic region 
      [(34.96+/-5.65)%] in the miR-26a-5p group was significantly increased in comparison 
      with the control group [(23.28+/-3.03)%], while in the antimiR-26a-5p group 
      [(8.02+/-2.27)%] was significantly lower (P<0.05). The luciferase activity of the 
      Group A (0.46+/-0.06) was significantly lower than Group B (3.46+/-0.45) (P<0.05). 
      Compared with the control group, the expression levels of Wnt5a protein, 
      calmodulin kinase Ⅱ and protein kinase C proteins in the Group Ⅲ were 
      significantly decreased, while those in the GroupⅤ were significantly increased 
      (P<0.05). Conclusions: MicroRNA-26a-5p could promote osteogenic differentiation 
      of PPDLSC in vivo and in vitro, and its mechanism might be inhibiting the 
      activation of Wnt/Ca(2+) signaling pathway by targeting Wnt5a.
FAU - Zhang, K K
AU  - Zhang KK
AD  - Department of Orthodontics, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, China.
FAU - Geng, Y D
AU  - Geng YD
AD  - Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of 
      Zhengzhou University, Zhengzhou 450052, China.
FAU - Wang, S B
AU  - Wang SB
AD  - Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of 
      Zhengzhou University, Zhengzhou 450052, China.
FAU - Huo, L
AU  - Huo L
AD  - Department of Orthodontics, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (MIRN26A microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (WNT5A protein, human)
RN  - 0 (Wnt-5a Protein)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Cell Differentiation
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - *MicroRNAs/physiology
MH  - *Osteogenesis
MH  - *Periodontal Ligament/metabolism
MH  - Stem Cells
MH  - *Wnt-5a Protein/metabolism
OTO - NOTNLM
OT  - Infla- mmatory microenvironment
OT  - MicroRNAs
OT  - Osteogenic differentiation
OT  - Periodontal ligament stem cells
EDAT- 2019/10/15 06:00
MHDA- 2019/11/26 06:00
CRDT- 2019/10/15 06:00
PHST- 2019/10/15 06:00 [entrez]
PHST- 2019/10/15 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
AID - 10.3760/cma.j.issn.1002-0098.2019.10.003 [doi]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2019 Oct 9;54(10):662-669. doi: 
      10.3760/cma.j.issn.1002-0098.2019.10.003.