PMID- 31609809
OWN - NLM
STAT- MEDLINE
DCOM- 20210614
LR  - 20210614
IS  - 1473-5709 (Electronic)
IS  - 0959-8278 (Linking)
VI  - 29
IP  - 2
DP  - 2020 Mar
TI  - Circular RNA Pleiotrophin promotes carcinogenesis in glioma via regulation of 
      microRNA-122/SRY-box transcription factor 6 axis.
PG  - 165-173
LID - 10.1097/CEJ.0000000000000535 [doi]
AB  - BACKGROUND: Circular RNAs (circRNAs) are recently identified as gene regulators 
      in mammals and play important roles in carcinogenesis of cancer. For example, 
      circRNA_PTN has been recognized as a biomarker of human cancer and is 
      overexpressed in glioma. The molecular function of circRNA_PTN and its downstream 
      targets in glioma, however, remains elusive. METHODS: Quantitative polymerase 
      chain reaction analysis was used to measure the expression of circular RNA 
      pleiotrophin (circ_PTN) and miR-122. 
      3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, propidium 
      iodide and Annexin-V/propidium iodide assay were performed to determine cell 
      proliferation and apoptosis of glioma cells. Circular RNA Interactome and 
      TargetScan were used to predict the potential microRNA targeting of circ_PTN and 
      the potential targets of miR-122, respectively. Luciferase activity assay was 
      used to validate these interactions. Downstream molecular mechanisms, including 
      SRY-box transcription factor 6 (SOX6), extracellular regulated protein kinases 
      (ERK), Cyclin D1, B-cell lymphoma-2 (BCL-2) and BCL2 associated X, apoptosis 
      regulator (BAX), were determined by western blot. RESULTS: Circ_PTN was 
      overexpressed in glioma cells, and its knockdown induced cell proliferation 
      inhibition, cell cycle arrest and apoptosis in glioma cells. The target microRNA 
      of circ_PTN was predicted to be miR-122, the expression of which was negatively 
      correlated with circ_PTN in glioma cells. Moreover, SOX6 was predicted as a 
      potential target of miR-122, and miR-122 overexpression decreased SOX6 
      expression. MiR-122 inhibitor reversed the tumor-suppressing effects of circ_PTN 
      knockdown, while overexpression of SOX6 impaired the miR-122 
      overexpression-induced cell growth inhibition and apoptosis. In addition, mitogen 
      activated kinase-like protein (MAPK)/ERK pathway was involved in 
      circ_PTN/miR-122/SOX6 axis. CONCLUSIONS: Circ_PTN acted as a sponge of miR-122 
      and upregulated miR-122 target SOX6, thus promoting carcinogenesis of glioma 
      cells.
FAU - Chen, Chen
AU  - Chen C
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, 
      Suzhou.
AD  - Department of Neurosurgery, Yancheng City No. 1 People's Hospital, Yancheng.
FAU - Deng, Lu
AU  - Deng L
AD  - Department of Orthopedics, Yancheng City No. 1 People's Hospital, Yancheng, P.R. 
      China.
FAU - Nie, De-Kang
AU  - Nie DK
AD  - Department of Neurosurgery, Yancheng City No. 1 People's Hospital, Yancheng.
FAU - Jia, Feng
AU  - Jia F
AD  - Department of Neurosurgery, Yancheng City No. 1 People's Hospital, Yancheng.
FAU - Fu, Lin-Shan
AU  - Fu LS
AD  - Department of Neurosurgery, Yancheng City No. 1 People's Hospital, Yancheng.
FAU - Wan, Zheng-Qiang
AU  - Wan ZQ
AD  - Department of Neurosurgery, Yancheng City No. 1 People's Hospital, Yancheng.
FAU - Lan, Qing
AU  - Lan Q
AD  - Department of Neurosurgery, The Second Affiliated Hospital of Soochow University, 
      Suzhou.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Cancer Prev
JT  - European journal of cancer prevention : the official journal of the European 
      Cancer Prevention Organisation (ECP)
JID - 9300837
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytokines)
RN  - 0 (MIRN122 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (SOX6 protein, human)
RN  - 0 (SOXD Transcription Factors)
RN  - 134034-50-7 (pleiotrophin)
SB  - IM
MH  - Apoptosis/genetics
MH  - Brain Neoplasms/*genetics/pathology
MH  - Carcinogenesis/genetics
MH  - Carrier Proteins/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Cytokines/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Glioma/*genetics/pathology
MH  - Humans
MH  - MAP Kinase Signaling System/genetics
MH  - MicroRNAs/*metabolism
MH  - RNA, Circular/genetics/*metabolism
MH  - SOXD Transcription Factors/*genetics
EDAT- 2019/10/15 06:00
MHDA- 2021/06/16 06:00
CRDT- 2019/10/15 06:00
PHST- 2019/10/15 06:00 [pubmed]
PHST- 2021/06/16 06:00 [medline]
PHST- 2019/10/15 06:00 [entrez]
AID - 00008469-202003000-00010 [pii]
AID - 10.1097/CEJ.0000000000000535 [doi]
PST - ppublish
SO  - Eur J Cancer Prev. 2020 Mar;29(2):165-173. doi: 10.1097/CEJ.0000000000000535.