PMID- 31610144
OWN - NLM
STAT- MEDLINE
DCOM- 20200810
LR  - 20200810
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Linking)
VI  - 458
IP  - 1
DP  - 2020 Feb 1
TI  - Amphiregulin mediates the hormonal regulation on Rspondin-1 expression in the 
      mammary gland.
PG  - 43-51
LID - S0012-1606(19)30232-5 [pii]
LID - 10.1016/j.ydbio.2019.10.006 [doi]
AB  - The steroid hormones are instrumental for the growth of mammary epithelial cells. 
      Our previous study indicates that hormones regulate the expression of Rspondin-1 
      (Rspo1). Yet, the regulatory mechanism remains unknown. In the current study, we 
      identify Amphiregulin (Areg) as a novel upstream regulator of Rspo1 expression 
      mediating the hormonal influence. In response to hormonal signaling, Areg 
      emanating from estrogen receptor (ER)-positive luminal cells, induce the 
      expression of Rspo1 in ER-negative luminal cells. The paracrine action of Areg on 
      Rspo1 expression is dependent on Egfr. Our data reveal a novel 
      Estrogen-Areg-Rspo1 regulatory axis in the mammary gland, providing new evidence 
      for the orchestrated action of systemic hormones and local growth factors.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Cai, Cheguo
AU  - Cai C
AD  - State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell 
      Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of 
      Sciences, Shanghai, 200031, China; Medical Research Institute, Wuhan University, 
      Wuhan, 430071, China.
FAU - Geng, Ajun
AU  - Geng A
AD  - State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell 
      Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of 
      Sciences, Shanghai, 200031, China.
FAU - Wang, Mengran
AU  - Wang M
AD  - Key Laboratory of Computational Biology, CAS-MPG Partner Institute for 
      Computational Biology, Shanghai Institutes for Biological Sciences, Chinese 
      Academy of Sciences, Shanghai, 200031, China.
FAU - Yang, Li
AU  - Yang L
AD  - Key Laboratory of Computational Biology, CAS-MPG Partner Institute for 
      Computational Biology, Shanghai Institutes for Biological Sciences, Chinese 
      Academy of Sciences, Shanghai, 200031, China.
FAU - Yu, Qing Cissy
AU  - Yu QC
AD  - State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell 
      Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of 
      Sciences, Shanghai, 200031, China. Electronic address: cissyyu@sibcb.ac.cn.
FAU - Zeng, Yi Arial
AU  - Zeng YA
AD  - State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell 
      Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of 
      Sciences, Shanghai, 200031, China. Electronic address: yzeng@sibcb.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191011
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Amphiregulin)
RN  - 0 (Areg protein, mouse)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RSPO1 protein, mouse)
RN  - 0 (Thrombospondins)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - DA87705X9K (Erlotinib Hydrochloride)
RN  - EC 2.7.10.1 (EGFR protein, mouse)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Amphiregulin/genetics/*physiology
MH  - Animals
MH  - Cells, Cultured
MH  - ErbB Receptors/antagonists & inhibitors/physiology
MH  - Erlotinib Hydrochloride/pharmacology
MH  - Estradiol/pharmacology/*physiology
MH  - Estrous Cycle/genetics/*physiology
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Mammary Glands, Animal/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Primary Cell Culture
MH  - Progesterone/pharmacology/*physiology
MH  - RNA, Small Interfering/genetics
MH  - Thrombospondins/*biosynthesis/genetics
MH  - Transcriptome
OTO - NOTNLM
OT  - Areg
OT  - Egfr
OT  - Estrogen
OT  - Hormone
OT  - Rspo1
COIS- Declaration of competing interest The authors declare no competing financial 
      interest.
EDAT- 2019/10/15 06:00
MHDA- 2020/08/11 06:00
CRDT- 2019/10/15 06:00
PHST- 2019/04/12 00:00 [received]
PHST- 2019/09/23 00:00 [revised]
PHST- 2019/10/08 00:00 [accepted]
PHST- 2019/10/15 06:00 [pubmed]
PHST- 2020/08/11 06:00 [medline]
PHST- 2019/10/15 06:00 [entrez]
AID - S0012-1606(19)30232-5 [pii]
AID - 10.1016/j.ydbio.2019.10.006 [doi]
PST - ppublish
SO  - Dev Biol. 2020 Feb 1;458(1):43-51. doi: 10.1016/j.ydbio.2019.10.006. Epub 2019 
      Oct 11.