PMID- 31610189
OWN - NLM
STAT- MEDLINE
DCOM- 20191217
LR  - 20191217
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 238
DP  - 2019 Dec 1
TI  - Dendritic cells aggregate inflammation in experimental osteoarthritis through a 
      toll-like receptor (TLR)-dependent machinery response to challenges.
PG  - 116920
LID - S0024-3205(19)30847-1 [pii]
LID - 10.1016/j.lfs.2019.116920 [doi]
AB  - AIMS: Dendritic cells (DCs) and Toll-like receptor (TLR) participate in mediating 
      inflammation process. However, the functional role of TLR expressed on DCs in 
      osteoarthritis (OA) development has not been defined yet. The purpose of this 
      study was to investigate the role and mechanism of TLR and DCs in the progression 
      of experimental osteoarthritis (OA). MATERIALS AND METHODS: Experimental OA model 
      was induced by iodoacetate injection. Expressions of toll-like receptors in DCs 
      of OA mice were detected by qRT-PCR and flow cytometry. TLR agonists 
      lipopolysaccharide (LPS) and R848 or TLR antagonist FP7 were used, and the levels 
      of TLRs and inflammatory cytokines were examined by qRT-PCR and ELISA. KEY 
      FINDINGS: The expression levels of TLR family members were increased in DCs 
      derived from synovial fluid of OA mice compared with the sham mice. In vitro, OA 
      mice-derived DCs had increased production of inflammatory cytokine after TLR 
      agonists LPS and R848 challenge, while TLR challenges did not affect DCs 
      maturation. Inhibition of TLR by TLR antagonist FP7 blocked TLR 
      challenges-induced increased inflammation in DCs. In mice, administration of FP7 
      attenuated LPS-induced inflammatory response and OA condition. SIGNIFICANCE: 
      Increased TLR expression in OA-derived DCs contributes to the inflammation 
      condition and potentially acts as a therapeutic target for osteoarthritis.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Nie, Fengfeng
AU  - Nie F
AD  - Department of Orthopedics, Linyi Central Hospital, Linyi, Shandong, 276400, 
      China.
FAU - Ding, Fei
AU  - Ding F
AD  - Department of Orthopedics, Sheyang County People's Hospital, Yancheng, Jiangsu, 
      224300, China.
FAU - Chen, Bo
AU  - Chen B
AD  - Department of Orthopedics, Linyi Central Hospital, Linyi, Shandong, 276400, 
      China.
FAU - Huang, Shouguo
AU  - Huang S
AD  - Department of Orthopedics, Linyi Central Hospital, Linyi, Shandong, 276400, 
      China.
FAU - Liu, Qingbai
AU  - Liu Q
AD  - Department of Orthopedics, Lianshui County People's Hospital, The Affiliated 
      Lianshui County People's Hospital of Kangda College of Nanjing Medical 
      University, Huai'an, Jiangsu, 223400, China. Electronic address: 
      liu678vip@163.com.
FAU - Xu, Changming
AU  - Xu C
AD  - Department of Orthopedics, Lianshui County People's Hospital, The Affiliated 
      Lianshui County People's Hospital of Kangda College of Nanjing Medical 
      University, Huai'an, Jiangsu, 223400, China. Electronic address: sea009@126.com.
LA  - eng
PT  - Journal Article
DEP - 20191011
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Animals
MH  - Arthritis, Rheumatoid/*complications
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - Inflammation/*etiology/metabolism/pathology
MH  - Lipopolysaccharides/toxicity
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoarthritis/*complications
MH  - Toll-Like Receptors/*metabolism
OTO - NOTNLM
OT  - Dendritic cells
OT  - Inflammation
OT  - Osteoarthritis
OT  - Toll-like receptor
EDAT- 2019/10/15 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/10/15 06:00
PHST- 2019/08/16 00:00 [received]
PHST- 2019/09/26 00:00 [revised]
PHST- 2019/09/27 00:00 [accepted]
PHST- 2019/10/15 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/10/15 06:00 [entrez]
AID - S0024-3205(19)30847-1 [pii]
AID - 10.1016/j.lfs.2019.116920 [doi]
PST - ppublish
SO  - Life Sci. 2019 Dec 1;238:116920. doi: 10.1016/j.lfs.2019.116920. Epub 2019 Oct 
      11.