PMID- 31611386
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20200622
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 116
IP  - 44
DP  - 2019 Oct 29
TI  - Highly ABA-Induced 1 (HAI1)-Interacting protein HIN1 and drought 
      acclimation-enhanced splicing efficiency at intron retention sites.
PG  - 22376-22385
LID - 10.1073/pnas.1906244116 [doi]
AB  - The Highly ABA-Induced 1 (HAI1) protein phosphatase is a central component of 
      drought-related signaling. A screen for HAI1-interacting proteins identified 
      HAI1-Interactor 1 (HIN1), a nuclear protein of unknown function which could be 
      dephosphorylated by HAI1 in vitro. HIN1 colocalization and interaction with 
      serine-arginine rich (SR) splicing factors and appearance of nuclear 
      speckle-localized HIN1 during low water potential (psi(w)) stress suggested a 
      pre-mRNA splicing-related function. RNA sequencing of Arabidopsis Col-0 wild type 
      identified more than 500 introns where moderate severity low psi(w) altered intron 
      retention (IR) frequency. Surprisingly, nearly 90% of these had increased 
      splicing efficiency (decreased IR) during stress. For one-third of these introns, 
      ectopic HIN1 expression (35S:HIN1) in unstressed plants mimicked the increased 
      splicing efficiency seen in stress-treated wild type. HIN1 bound to a GAA-repeat, 
      Exonic Splicing Enhancer-like RNA motif enriched in flanking sequence around 
      HIN1-regulated introns. Genes with stress and HIN1-affected splicing efficiency 
      were enriched for abiotic stress and signaling-related functions. The 35S:HIN1 
      plants had enhanced growth maintenance during low psi(w), while hin1 mutants had 
      reduced growth, further indicating the role of HIN1 in drought response. HIN1 is 
      annotated as an MYB/SANT domain protein but has limited homology to other 
      MYB/SANT proteins and is not related to known yeast or metazoan RNA-binding 
      proteins or splicing regulators. Together these data identify HIN1 as a 
      plant-specific RNA-binding protein, show a specific effect of drought acclimation 
      to promote splicing efficiency of IR-prone introns, and also discover HAI1-HIN1 
      interaction and dephosphorylation that connects stress signaling to splicing 
      regulation.
CI  - Copyright (c) 2019 the Author(s). Published by PNAS.
FAU - Chong, Geeng Loo
AU  - Chong GL
AD  - Institute of Plant and Microbial Biology, Academia Sinica, 115 Taipei, Taiwan.
AD  - Molecular and Biological Agricultural Sciences Program, Taiwan International 
      Graduate Program, National Chung-Hsing University, 402 Taichung, Taiwan, and 
      Academia Sinica, 115 Taipei, Taiwan.
AD  - Graduate Institute of Biotechnology, Biotechnology Center, National Chung-Hsing 
      University, 402 Taichung, Taiwan.
FAU - Foo, Mung Hsia
AU  - Foo MH
AD  - Institute of Plant and Microbial Biology, Academia Sinica, 115 Taipei, Taiwan.
FAU - Lin, Wen-Dar
AU  - Lin WD
AD  - Institute of Plant and Microbial Biology, Academia Sinica, 115 Taipei, Taiwan.
FAU - Wong, Min May
AU  - Wong MM
AD  - Institute of Plant and Microbial Biology, Academia Sinica, 115 Taipei, Taiwan.
AD  - Molecular and Biological Agricultural Sciences Program, Taiwan International 
      Graduate Program, National Chung-Hsing University, 402 Taichung, Taiwan, and 
      Academia Sinica, 115 Taipei, Taiwan.
AD  - Graduate Institute of Biotechnology, Biotechnology Center, National Chung-Hsing 
      University, 402 Taichung, Taiwan.
FAU - Verslues, Paul E
AU  - Verslues PE
AUID- ORCID: 0000-0001-5340-6010
AD  - Institute of Plant and Microbial Biology, Academia Sinica, 115 Taipei, Taiwan; 
      paulv@gate.sinica.edu.tw.
AD  - Molecular and Biological Agricultural Sciences Program, Taiwan International 
      Graduate Program, National Chung-Hsing University, 402 Taichung, Taiwan, and 
      Academia Sinica, 115 Taipei, Taiwan.
AD  - Biotechnology Center, National Chung-Hsing University, 402 Taichung, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191014
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (AT3G11290 protein, Arabidopsis)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Arabidopsis
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Droughts
MH  - Eukaryotic Initiation Factor-3/genetics/*metabolism
MH  - Gene Expression Regulation, Plant
MH  - Introns
MH  - *Osmotic Pressure
MH  - *RNA Splicing
MH  - RNA-Binding Proteins/genetics/*metabolism
PMC - PMC6825267
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - HAI1 protein phosphatase
OT  - MYB/SANT domain
OT  - alternative splicing
OT  - intron retention
COIS- The authors declare no competing interest.
EDAT- 2019/10/16 06:00
MHDA- 2020/04/14 06:00
PMCR- 2019/10/14
CRDT- 2019/10/16 06:00
PHST- 2019/10/16 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
PHST- 2019/10/16 06:00 [entrez]
PHST- 2019/10/14 00:00 [pmc-release]
AID - 1906244116 [pii]
AID - 201906244 [pii]
AID - 10.1073/pnas.1906244116 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2019 Oct 29;116(44):22376-22385. doi: 
      10.1073/pnas.1906244116. Epub 2019 Oct 14.