PMID- 31611441
OWN - NLM
STAT- MEDLINE
DCOM- 20200220
LR  - 20220410
IS  - 0974-5130 (Electronic)
IS  - 0377-4929 (Linking)
VI  - 62
IP  - 4
DP  - 2019 Oct-Dec
TI  - Morphological characterization and molecular profiling of malignant pericardial 
      effusion in patients with pulmonary adenocarcinoma.
PG  - 566-571
LID - 10.4103/IJPM.IJPM_69_19 [doi]
AB  - CONTEXT: Malignant pericardial effusions (MPCEs) is a common complication 
      observed in advanced pulmonary adenocarcinoma. In such cases, investigating 
      molecular alterations can have significant therapeutic implication in determining 
      anticancer drugs. AIM: The objective was to evaluate the significance of cell 
      block technique in the diagnosis of MPCE and further investigate the 
      morphological and molecular profiles of MPCE in patients with pulmonary 
      adenocarcinoma. SETTING AND DESIGN: Cytopathological and molecular profiles of 19 
      MPCE cases in patients with pulmonary adenocarcinoma were retrospectively 
      analyzed. The control group consisted of 14 malignant pleural effusion (MPE) 
      cases in patients with pulmonary adenocarcinoma. MATERIALS AND METHODS: 
      Anaplastic lymphoma kinase (ALK) and tyrosine-protein kinase Met (C-MET) 
      expression was evaluated by fluorescence in situ hybridization (FISH). Epithelial 
      growth factor receptor (EGFR) and K-Ras (KRAS) mutations were detected by ARMS 
      real-time polymerase chain reaction (RT-PCR). STATISTICAL ANALYSIS USED: 
      Associations between MPCE and MPE were analyzed using Fisher's exact test. 
      RESULTS: MPCE was found to have micropapillary and solid pattern predominant with 
      mucin secretion compared to acinar patterns, as seen in MPE. Seventeen MPCE cases 
      (89.5%) and all MPE cases (100%) underwent molecular analysis. Mutations in EGFR 
      and KRAS, ALK rearrangement, and C-MET amplification were observed in MPCE and 
      MPE with statistical differences. Additionally, two MPCE cases demonstrated EGFR 
      T790M mutation and multiple insertions at L858. CONCLUSIONS: MPCE shows 
      micropapillary and solid cytological patterns predominant with mucin secretion. 
      MPCE are suitable to analyze oncogenic mutations and to develop targeted therapy 
      for patients with pulmonary adenocarcinoma. Further molecular investigations may 
      reveal novel molecular alterations.
FAU - Zhou, Bing
AU  - Zhou B
AD  - Department of Oncology, The First Affiliated Hospital of Nanchang University, 
      Nanchang; Department of Pathology, The Affiliated Jiujiang Hospital of Nanchang 
      University, Jiujiang, Jiangxi, P.R. China.
FAU - Li, Lin
AU  - Li L
AD  - Department of Gerontology, Wuhan No. 1 Hospital, Wuhan, Hubei, P.R. China.
FAU - Shi, Dayou
AU  - Shi D
AD  - Department of Oncology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, P.R. China.
FAU - Jiang, Tao
AU  - Jiang T
AD  - Department of Pathology, The Affiliated Jiujiang Hospital of Nanchang University, 
      Jiujiang, Jiangxi, P.R. China.
FAU - Xue, Guohui
AU  - Xue G
AD  - Department of Oncology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, P.R. China.
FAU - Xiong, Jianping
AU  - Xiong J
AD  - Department of Oncology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, P.R. China.
LA  - eng
PT  - Journal Article
PL  - India
TA  - Indian J Pathol Microbiol
JT  - Indian journal of pathology & microbiology
JID - 7605904
RN  - 0 (KRAS protein, human)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
SB  - IM
MH  - Adenocarcinoma of Lung/*genetics/pathology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - ErbB Receptors/genetics
MH  - Female
MH  - *Gene Expression Profiling
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Pericardial Effusion/*cytology/*genetics
MH  - Pleural Neoplasms/diagnosis/*genetics
MH  - Proto-Oncogene Proteins p21(ras)/genetics
MH  - Retrospective Studies
OTO - NOTNLM
OT  - Cytology
OT  - malignant pericardial effusion
OT  - oncogene mutation
OT  - pulmonary adenocarcinoma
EDAT- 2019/10/16 06:00
MHDA- 2020/02/23 06:00
CRDT- 2019/10/16 06:00
PHST- 2019/10/16 06:00 [entrez]
PHST- 2019/10/16 06:00 [pubmed]
PHST- 2020/02/23 06:00 [medline]
AID - IndianJPatholMicrobiol_2019_62_4_566_269079 [pii]
AID - 10.4103/IJPM.IJPM_69_19 [doi]
PST - ppublish
SO  - Indian J Pathol Microbiol. 2019 Oct-Dec;62(4):566-571. doi: 
      10.4103/IJPM.IJPM_69_19.