PMID- 31614634 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200928 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 11 IP - 10 DP - 2019 Oct 11 TI - Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-small Cell Lung Cancer. LID - 10.3390/cancers11101538 [doi] LID - 1538 AB - Background: Recent in vitro studies have indicated that irisin inhibits proliferation, migration and epithelial-mesenchymal transition. Irisin expression has not been studied in tumour tissues of non-small cell lung cancer (NSCLC) patients yet. The aim of the study was to determine the irisin expression in NSCLCs in comparison to the clinicopathological factors and expression of TTF-1, p63 and Ki-67. Material and methods: Tissue microarrays with 729 NSCLC and 140 non-malignant lung tissue (NMLT) were used to perform immunohistochemical reactions. Laser Capture Microdissection (LCM) was used to collect cancer and stromal cells from NSCLCs. FNDC5 expression was tested for LCM samples, 75 NSCLCs and 25 NMLTs with the RT-PCR technique. Western-blot, immunofluorescence reaction and RT-PCR assays were performed on lung cancer cell lines. Results: Irisin expression was observed in NSCLC cancer cells and stromal fibroblasts. In cancer cells, irisin expression was decreased in higher grades (G) of malignancy, tumour size (T) and according to lymph node metastasis. In stromal cells, irisin expression was increased in higher G and advanced T. A shorter overall survival was observed in patients with higher irisin expression in NSCLC stromal cells. Conclusions: Irisin expression in stromal fibroblasts may influence cancer cell proliferation and may be a prognostic factor for survival in NSCLC. FAU - Nowinska, Katarzyna AU - Nowinska K AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. katarzyna.nowinska@umed.wroc.pl. FAU - Jablonska, Karolina AU - Jablonska K AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. karolina.jablonska@umed.wroc.pl. FAU - Pawelczyk, Konrad AU - Pawelczyk K AD - Department of Thoracic Surgery, Wroclaw Medical University, 53-439 Wroclaw, Poland. kopaw@wp.pl. AD - Department of Thoracic Surgery, Lower Silesian Centre of Lung Diseases, 53-439 Wroclaw, Poland. kopaw@wp.pl. FAU - Piotrowska, Aleksandra AU - Piotrowska A AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. FAU - Partynska, Aleksandra AU - Partynska A AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. ola.partynska@onet.pl. FAU - Gomulkiewicz, Agnieszka AU - Gomulkiewicz A AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. agnieszka.gomulkiewicz@umed.wroc.pl. FAU - Ciesielska, Urszula AU - Ciesielska U AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. urszula.ciesielska@umed.wroc.pl. FAU - Katnik, Ewa AU - Katnik E AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. ewa.sadowska@interia.pl. FAU - Grzegrzolka, Jedrzej AU - Grzegrzolka J AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. jedrzej.grzegrzolka@gmail.com. FAU - Glatzel-Plucinska, Natalia AU - Glatzel-Plucinska N AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. n.m.glatzel@gmail.com. FAU - Ratajczak-Wielgomas, Katarzyna AU - Ratajczak-Wielgomas K AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. katarzyna.ratajczak-wielgomas@umed.wroc.pl. FAU - Podhorska-Okolow, Marzenna AU - Podhorska-Okolow M AD - Division of Ultrastructure Research, Wroclaw Medical University, 50-368 Wroclaw, Poland. marzenna.podhorska-okolow@umed.wroc.pl. FAU - Dziegiel, Piotr AU - Dziegiel P AD - Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland. piotr.dziegiel@umed.wroc.pl. AD - Department of Physiotherapy, Wroclaw University School of Physical Education, 51-612 Wroclaw, Poland. piotr.dziegiel@umed.wroc.pl. LA - eng GR - STM.A110.17.026/Uniwersytet Medyczny im. Piastow Slaskich we Wroclawiu/ PT - Journal Article DEP - 20191011 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC6826442 OTO - NOTNLM OT - Ki-67 OT - TTF-1 OT - cancer OT - cancer-associated fibroblasts OT - irisin OT - non-small cell lung cancer OT - p63 COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2019/10/17 06:00 MHDA- 2019/10/17 06:01 PMCR- 2019/10/11 CRDT- 2019/10/17 06:00 PHST- 2019/07/30 00:00 [received] PHST- 2019/09/04 00:00 [revised] PHST- 2019/10/10 00:00 [accepted] PHST- 2019/10/17 06:00 [entrez] PHST- 2019/10/17 06:00 [pubmed] PHST- 2019/10/17 06:01 [medline] PHST- 2019/10/11 00:00 [pmc-release] AID - cancers11101538 [pii] AID - cancers-11-01538 [pii] AID - 10.3390/cancers11101538 [doi] PST - epublish SO - Cancers (Basel). 2019 Oct 11;11(10):1538. doi: 10.3390/cancers11101538.