PMID- 31614977
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 11
IP  - 10
DP  - 2019 Oct 14
TI  - Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and 
      Electrostatic Interactions.
LID - 10.3390/pharmaceutics11100530 [doi]
LID - 530
AB  - Gelatin Type A (GA) and sodium alginate (SA) complexes were explored to 
      encapsulate epidermal growth factor (EGF), and thereby to circumvent its 
      proteolytic degradation upon topical application to chronic wounds. Phase 
      diagrams were constructed based on turbidity as a function of GA to SA ratio and 
      pH. Various GA-SA mixtures were compared for polydispersity index, zeta 
      potential, Z-average, and ATR-FTIR spectra. Trypsin digestion and human dermal 
      fibroblast scratch wound assay were done to evaluate the effects of EGF 
      encapsulation. The onset pH values for coacervation and precipitation were closer 
      together in high molecular weight GA (HWGA)-SA reaction mixtures than in low 
      molecular weight GA (LWGA)-SA, which was attributed to strong H-bonding 
      interactions between HWGA and SA probed by ATR-FTIR. EGF incorporation in both 
      HWGA-SA precipitates and LWGA-SA coacervates below the isoelectric point of EGF, 
      but not above it, suggests the contribution of electrostatic interactions between 
      EGF and SA. EGF encapsulated in LWGA-SA coacervates was effectively protected 
      from trypsin digestion and showed better in vitro scratch wound activity compared 
      to free EGF. LWGA-SA coacervates are suggested as a novel delivery system for 
      topical application of EGF to chronic wounds.
FAU - Jeong, Seonghee
AU  - Jeong S
AD  - College of Pharmacy, CHA University, Seongnam-si, Gyeonggi-do 463-400, Korea. 
      dooblue@hanmail.net.
FAU - Kim, ByungWook
AU  - Kim B
AD  - College of Pharmacy, CHA University, Seongnam-si, Gyeonggi-do 463-400, Korea.
FAU - Lau, Hui-Chong
AU  - Lau HC
AD  - College of Pharmacy, CHA University, Seongnam-si, Gyeonggi-do 463-400, Korea. 
      huichong.lau@gmail.com.
FAU - Kim, Aeri
AU  - Kim A
AD  - College of Pharmacy, CHA University, Seongnam-si, Gyeonggi-do 463-400, Korea. 
      arkim@cha.ac.kr.
LA  - eng
GR  - NRF-2017R1D1A1B03031548/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20191014
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC6835588
OTO - NOTNLM
OT  - H-bonding interactions
OT  - alginate
OT  - coacervate
OT  - diabetic foot ulcer
OT  - electrostatic interactions
OT  - epidermal growth factor (EGF)
OT  - gelatin
OT  - polyelectrolyte complex
COIS- The authors declare no conflict of interest.
EDAT- 2019/10/17 06:00
MHDA- 2019/10/17 06:01
PMCR- 2019/10/01
CRDT- 2019/10/17 06:00
PHST- 2019/08/16 00:00 [received]
PHST- 2019/09/24 00:00 [revised]
PHST- 2019/10/11 00:00 [accepted]
PHST- 2019/10/17 06:00 [entrez]
PHST- 2019/10/17 06:00 [pubmed]
PHST- 2019/10/17 06:01 [medline]
PHST- 2019/10/01 00:00 [pmc-release]
AID - pharmaceutics11100530 [pii]
AID - pharmaceutics-11-00530 [pii]
AID - 10.3390/pharmaceutics11100530 [doi]
PST - epublish
SO  - Pharmaceutics. 2019 Oct 14;11(10):530. doi: 10.3390/pharmaceutics11100530.