PMID- 31617507
OWN - NLM
STAT- MEDLINE
DCOM- 20200421
LR  - 20200421
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 144
IP  - 22
DP  - 2019 Nov 4
TI  - A biomimetic fluorescent nanosensor based on imprinted polymers modified with 
      carbon dots for sensitive detection of alpha-fetoprotein in clinical samples.
PG  - 6760-6772
LID - 10.1039/c9an01065c [doi]
AB  - A biomimetic fluorescent nanosensor based on molecularly imprinted polymers 
      modified with carbon dots (CDs@MIPs) has been prepared for rapid, selective and 
      sensitive detection of alpha-fetoprotein (AFP) in clinical samples. The 
      nanosensor was produced using vinyl-functionalized CDs (V-CDs) as transducer 
      elements and support materials, AFP as the template protein, 
      N-isopropylacrylamide (NIPAM) and 4-vinylphenylbronic acid (VPBA) as the 
      thermo-responsive and pH-responsive monomer, respectively, and ammonium 
      peroxodisulphate (APS) and N,N'-methylene bisacrylamide (MBA) as the initiator 
      and cross-linker, respectively. The newly synthesized nanosensor was 
      characterized by FT-IR, TEM, XRD and elemental analysis, which unambiguously 
      confirmed the successful formation of the nanosensor. The fluorescence quenching 
      degree of CDs@MIPs exhibited a good linear response to AFP in a concentration 
      range of 10 to 100 ng mL-1, the limit of detection (LOD) of 0.474 ng mL-1, and 
      high recoveries at three spiking levels of AFP ranging from 97.05% to 102.00%, 
      with relative standard deviations (RSDs) below 4.2% being obtained. Moreover, the 
      proposed CDs@MIPs were successfully exploited to detect AFP in human serum 
      samples. This study successfully established a novel method for rapid, 
      convenient, and highly sensitive and selective detection of AFP, which provides 
      new ideas for the detection of tumor markers.
FAU - Sun, Chenghong
AU  - Sun C
AD  - School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, China. 
      chenlina@njmu.edu.cnl.
FAU - Pan, Linli
AU  - Pan L
FAU - Zhang, Lei
AU  - Zhang L
FAU - Huang, Jiaojiao
AU  - Huang J
FAU - Yao, Dandan
AU  - Yao D
FAU - Wang, Chong-Zhi
AU  - Wang CZ
FAU - Zhang, Yu
AU  - Zhang Y
FAU - Jiang, Nan
AU  - Jiang N
FAU - Chen, Lina
AU  - Chen L
FAU - Yuan, Chun-Su
AU  - Yuan CS
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (4-vinylphenyl boronic acid)
RN  - 0 (AFP protein, human)
RN  - 0 (Acrylamides)
RN  - 0 (Boronic Acids)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polymers)
RN  - 0 (Sulfates)
RN  - 0 (Vinyl Compounds)
RN  - 0 (alpha-Fetoproteins)
RN  - 7440-44-0 (Carbon)
RN  - B7GFF17L9U (N-isopropylacrylamide)
RN  - EDK4RIE19C (N,N'-methylenebisacrylamide)
SB  - IM
MH  - Acrylamides/chemistry
MH  - Biomimetic Materials/*chemistry
MH  - Boronic Acids/chemistry
MH  - Carbon/chemistry
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - Limit of Detection
MH  - Molecular Imprinting/methods
MH  - Polymerization
MH  - Polymers/chemical synthesis/*chemistry
MH  - Quantum Dots/*chemistry
MH  - Spectrometry, Fluorescence/methods
MH  - Sulfates/chemistry
MH  - Vinyl Compounds/chemistry
MH  - alpha-Fetoproteins/*analysis
EDAT- 2019/10/17 06:00
MHDA- 2020/04/22 06:00
CRDT- 2019/10/17 06:00
PHST- 2019/10/17 06:00 [pubmed]
PHST- 2020/04/22 06:00 [medline]
PHST- 2019/10/17 06:00 [entrez]
AID - 10.1039/c9an01065c [doi]
PST - ppublish
SO  - Analyst. 2019 Nov 4;144(22):6760-6772. doi: 10.1039/c9an01065c.