PMID- 31618225
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 10
DP  - 2019
TI  - Identification and characterisation of capidermicin, a novel bacteriocin produced 
      by Staphylococcus capitis.
PG  - e0223541
LID - 10.1371/journal.pone.0223541 [doi]
LID - e0223541
AB  - One hundred human-derived coagulase negative staphylococci (CoNS) were screened 
      for antimicrobial activity using agar-based deferred antagonism assays with a 
      range of indicator bacteria. Based on the findings of the screen and subsequent 
      well assays with cell free supernatants and whole cell extracts, one strain, 
      designated CIT060, was selected for further investigation. It was identified as 
      Staphylococcus capitis and herein we describe the purification and 
      characterisation of the novel bacteriocin that the strain produces. This 
      bacteriocin which we have named capidermicin was extracted from the cell-free 
      supernatant of S. capitis CIT060 and purified to homogeneity using reversed-phase 
      high performance liquid chromatography (RP-HPLC). Matrix-assisted laser 
      desorption/ionization time-of-flight (MALDI-TOF) mass spectrometric (MS) analysis 
      revealed that the capidermicin peptide has a mass of 5,464 Da. Minimal inhibitory 
      concentration (MIC) experiments showed that capidermicin was active in the 
      micro-molar range against all the Gram-positive bacteria that were tested. 
      Antimicrobial activity was retained over a range of pHs (2-11) and temperatures 
      (10-121 degrees C x 15 mins). The draft genome sequence of S. capitis CIT060 was 
      determined and the genes predicted to be involved in the biosynthesis of 
      capidermicin were identified. These genes included the predicted capidermicin 
      precursor gene, and genes that are predicted to encode a membrane transporter, an 
      immunity protein and a transcriptional regulator. Homology searches suggest that 
      capidermicin is a novel member of the family of class II leaderless bacteriocins.
FAU - Lynch, David
AU  - Lynch D
AD  - Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland.
FAU - O'Connor, Paula M
AU  - O'Connor PM
AD  - Teagasc Food Research Centre, Moorepark, Fermoy, Cork, Ireland.
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Cotter, Paul D
AU  - Cotter PD
AD  - Teagasc Food Research Centre, Moorepark, Fermoy, Cork, Ireland.
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
FAU - Hill, Colin
AU  - Hill C
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Microbiology, University College Cork, Cork, Ireland.
FAU - Field, Des
AU  - Field D
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
AD  - School of Microbiology, University College Cork, Cork, Ireland.
FAU - Begley, Maire
AU  - Begley M
AUID- ORCID: 0000-0003-0067-8818
AD  - Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland.
AD  - APC Microbiome Ireland, University College Cork, Cork, Ireland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191016
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacteriocins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacteriocins/analysis/*biosynthesis/chemistry
MH  - Base Sequence
MH  - Chromatography, Reverse-Phase
MH  - Genome, Bacterial
MH  - Humans
MH  - Mass Spectrometry
MH  - Microbial Sensitivity Tests
MH  - Models, Molecular
MH  - Open Reading Frames
MH  - Protein Conformation
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Staphylococcal Infections/microbiology
MH  - Staphylococcus capitis/drug effects/genetics/*metabolism
MH  - Whole Genome Sequencing
PMC - PMC6795431
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/10/17 06:00
MHDA- 2020/03/17 06:00
PMCR- 2019/10/16
CRDT- 2019/10/17 06:00
PHST- 2019/05/28 00:00 [received]
PHST- 2019/09/22 00:00 [accepted]
PHST- 2019/10/17 06:00 [entrez]
PHST- 2019/10/17 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
PHST- 2019/10/16 00:00 [pmc-release]
AID - PONE-D-19-15137 [pii]
AID - 10.1371/journal.pone.0223541 [doi]
PST - epublish
SO  - PLoS One. 2019 Oct 16;14(10):e0223541. doi: 10.1371/journal.pone.0223541. 
      eCollection 2019.