PMID- 31618236 OWN - NLM STAT- MEDLINE DCOM- 20200316 LR - 20231014 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 14 IP - 10 DP - 2019 TI - Macroepidemiological aspects of porcine reproductive and respiratory syndrome virus detection by major United States veterinary diagnostic laboratories over time, age group, and specimen. PG - e0223544 LID - 10.1371/journal.pone.0223544 [doi] LID - e0223544 AB - This project investigates the macroepidemiological aspects of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by veterinary diagnostic laboratories (VDLs) for the period 2007 through 2018. Standardized submission data and PRRSV real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) test results from porcine samples were retrieved from four VDLs representing 95% of all swine samples tested in NAHLN laboratories in the US. Anonymized data were retrieved and organized at the case level using SAS (SAS(R) Version 9.4, SAS(R) Institute, Inc., Cary, NC) with the use of PROC DATA, PROC MERGE, and PROC SQL scripts. The final aggregated and anonymized dataset comprised of 547,873 unique cases was uploaded to Power Business Intelligence-Power BI(R) (Microsoft Corporation, Redmond, Washington) to construct dynamic charts. The number of cases tested for PRRSV doubled from 2010 to 2018, with that increase mainly driven by samples typically used for monitoring purposes rather than diagnosis of disease. Apparent seasonal trends for the frequency of PRRSV detection were consistently observed with a higher percentage of positive cases occurring during fall or winter months and lower during summer months, perhaps due to increased testing associated with well-known seasonal occurrence of swine respiratory disease. PRRSV type 2, also known as North American genotype, accounted for 94.76% of all positive cases and was distributed across the US. PRRSV type 1, also known as European genotype, was geographically restricted and accounted for 2.15% of all positive cases. Co-detection of both strains accounted for 3.09% of the positive cases. Both oral fluid and processing fluid samples, had a rapid increase in the number of submissions soon after they were described in 2008 and 2017, respectively, suggesting rapid adoption of these specimens by the US swine industry for PRRSV monitoring in swine populations. As part of this project, a bio-informatics tool defined as Swine Disease Reporting System (SDRS) was developed. This tool has real-time capability to inform the US swine industry on the macroepidemiological aspects of PRRSV detection, and is easily adaptable for other analytes relevant to the swine industry. FAU - Trevisan, Giovani AU - Trevisan G AUID- ORCID: 0000-0002-4980-526X AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. FAU - Linhares, Leticia C M AU - Linhares LCM AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. FAU - Crim, Bret AU - Crim B AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. FAU - Dubey, Poonam AU - Dubey P AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. FAU - Schwartz, Kent J AU - Schwartz KJ AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. FAU - Burrough, Eric R AU - Burrough ER AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. FAU - Main, Rodger G AU - Main RG AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. FAU - Sundberg, Paul AU - Sundberg P AD - Swine Health Information Center, Ames, Iowa, United States of America. FAU - Thurn, Mary AU - Thurn M AD - Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, United States of America. FAU - Lages, Paulo T F AU - Lages PTF AD - Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, United States of America. FAU - Corzo, Cesar A AU - Corzo CA AD - Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, United States of America. FAU - Torrison, Jerry AU - Torrison J AD - Veterinary Population Medicine, University of Minnesota, Saint Paul, Minnesota, United States of America. FAU - Henningson, Jamie AU - Henningson J AD - College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Herrman, Eric AU - Herrman E AD - College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Hanzlicek, Gregg A AU - Hanzlicek GA AD - College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Raghavan, Ram AU - Raghavan R AD - College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Marthaler, Douglas AU - Marthaler D AD - College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Greseth, Jon AU - Greseth J AD - Veterinary & Biomedical Sciences Department, South Dakota State University, Brookings, South Dakota, United States of America. FAU - Clement, Travis AU - Clement T AD - Veterinary & Biomedical Sciences Department, South Dakota State University, Brookings, South Dakota, United States of America. FAU - Christopher-Hennings, Jane AU - Christopher-Hennings J AD - Veterinary & Biomedical Sciences Department, South Dakota State University, Brookings, South Dakota, United States of America. FAU - Linhares, Daniel C L AU - Linhares DCL AD - Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa, United States of America. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191016 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Animals MH - Clinical Laboratory Services MH - Geography, Medical MH - Laboratories, Hospital MH - Porcine Reproductive and Respiratory Syndrome/*diagnosis/epidemiology/*virology MH - *Porcine respiratory and reproductive syndrome virus/classification/genetics MH - Real-Time Polymerase Chain Reaction MH - Sensitivity and Specificity MH - Swine PMC - PMC6795434 COIS- Paul Sundberg holds the position of Executive Director of SHIC, which provided funding to this project. This does not alter our adherence to PLOS ONE policies on sharing data and materials. EDAT- 2019/10/17 06:00 MHDA- 2020/03/17 06:00 PMCR- 2019/10/16 CRDT- 2019/10/17 06:00 PHST- 2019/04/09 00:00 [received] PHST- 2019/09/23 00:00 [accepted] PHST- 2019/10/17 06:00 [entrez] PHST- 2019/10/17 06:00 [pubmed] PHST- 2020/03/17 06:00 [medline] PHST- 2019/10/16 00:00 [pmc-release] AID - PONE-D-19-10159 [pii] AID - 10.1371/journal.pone.0223544 [doi] PST - epublish SO - PLoS One. 2019 Oct 16;14(10):e0223544. doi: 10.1371/journal.pone.0223544. eCollection 2019.