PMID- 31620023
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 10
DP  - 2019
TI  - TRPV1 and TRPV1-Expressing Nociceptors Mediate Orofacial Pain Behaviors in a 
      Mouse Model of Orthodontic Tooth Movement.
PG  - 1207
LID - 10.3389/fphys.2019.01207 [doi]
LID - 1207
AB  - Orthodontic force produces mechanical irritation and inflammation in the 
      periodontium, which is inevitably accompanied by pain. Despite its prevalence, 
      treatment of orthodontic pain is ineffective. Elucidating underlying neural 
      mechanisms is critical to improving the management of orthodontic pain. We have 
      assessed the contribution of transient receptor potential vanilloid subtype 1 
      (TRPV1) and the TRPV1-expressing subset of nociceptive afferents to pain 
      behaviors induced by orthodontic force in mice. Microfocus X-ray computed 
      tomography analysis showed that application of an orthodontic force of 10 g to 
      the maxillary first molar produced reliable tooth movement in mice. Mouse grimace 
      scale (MGS) was evaluated as an indication of non-evoked spontaneous pain and 
      bite force (BF) was measured for assessing bite-evoked nocifensive behaviors. 
      Orthodontic force increased MGS and decreased BF, both of which were interpreted 
      as increased levels of pain. These behaviors peaked at 1d and returned near to 
      the sham level at 7d. Retrograde labeling and immunohistochemical assays showed 
      TRPV1-expressing peptidergic afferents are abundantly projected to the 
      periodontium. Direct injection of resiniferatoxin into trigeminal ganglia (TG) 
      decreased TRPV1-expressing afferents by half in the targeted region of TG. The 
      chemical ablation of TRPV1-expressing afferents significantly attenuated 
      orthodontic pain behaviors assessed by MGS and BF. Consistently, the knockout of 
      TRPV1 also attenuated orthodontic force-induced changes in MGS and BF. These 
      results suggest that TRPV1 and TRPV1-expressing trigeminal nociceptors constitute 
      a primary pathway mediating orthodontic pain behaviors in mice. This model will 
      be useful for mechanistic studies on orthodontic pain aimed at developing novel 
      approaches for painless orthodontics.
CI  - Copyright (c) 2019 Wang, Kim, Ali, Ong, Pae and Chung.
FAU - Wang, Sheng
AU  - Wang S
AD  - Program in Neuroscience, Center to Advance Chronic Pain Research, Department of 
      Neural and Pain Sciences, School of Dentistry, University of Maryland, Baltimore, 
      MD, United States.
FAU - Kim, Martin
AU  - Kim M
AD  - Program in Neuroscience, Center to Advance Chronic Pain Research, Department of 
      Neural and Pain Sciences, School of Dentistry, University of Maryland, Baltimore, 
      MD, United States.
FAU - Ali, Zayd
AU  - Ali Z
AD  - Program in Neuroscience, Center to Advance Chronic Pain Research, Department of 
      Neural and Pain Sciences, School of Dentistry, University of Maryland, Baltimore, 
      MD, United States.
FAU - Ong, Katherine
AU  - Ong K
AD  - Program in Neuroscience, Center to Advance Chronic Pain Research, Department of 
      Neural and Pain Sciences, School of Dentistry, University of Maryland, Baltimore, 
      MD, United States.
FAU - Pae, Eung-Kwon
AU  - Pae EK
AD  - Department of Orthodontic and Pediatric Dentistry, School of Dentistry, 
      University of Maryland, Baltimore, MD, United States.
FAU - Chung, Man-Kyo
AU  - Chung MK
AD  - Program in Neuroscience, Center to Advance Chronic Pain Research, Department of 
      Neural and Pain Sciences, School of Dentistry, University of Maryland, Baltimore, 
      MD, United States.
LA  - eng
GR  - R01 DE023846/DE/NIDCR NIH HHS/United States
GR  - R01 DE027731/DE/NIDCR NIH HHS/United States
PT  - Journal Article
DEP - 20190920
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
EIN - Front Physiol. 2019 Oct 22;10:1353. PMID: 31695629
PMC - PMC6763553
OTO - NOTNLM
OT  - TRPV1
OT  - behavioral assays
OT  - orthodontic tooth movement
OT  - peptidergic nociceptors
OT  - periodontium
OT  - trigeminal ganglia
EDAT- 2019/10/18 06:00
MHDA- 2019/10/18 06:01
PMCR- 2019/09/20
CRDT- 2019/10/18 06:00
PHST- 2019/05/21 00:00 [received]
PHST- 2019/09/04 00:00 [accepted]
PHST- 2019/10/18 06:00 [entrez]
PHST- 2019/10/18 06:00 [pubmed]
PHST- 2019/10/18 06:01 [medline]
PHST- 2019/09/20 00:00 [pmc-release]
AID - 10.3389/fphys.2019.01207 [doi]
PST - epublish
SO  - Front Physiol. 2019 Sep 20;10:1207. doi: 10.3389/fphys.2019.01207. eCollection 
      2019.