PMID- 31620148 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20231014 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 10 DP - 2019 TI - The Use of Pentaploid Crosses for the Introgression of Amblyopyrum muticum and D-Genome Chromosome Segments Into Durum Wheat. PG - 1110 LID - 10.3389/fpls.2019.01110 [doi] LID - 1110 AB - The wild relatives of wheat provide an important source of genetic variation for wheat improvement. Much of the work in the past aimed at transferring genetic variation from wild relatives into wheat has relied on the exploitation of the ph1b mutant, located on the long arm of chromosome 5B. This mutation allows homologous recombination to occur between chromosomes from related but different genomes, e.g. between the chromosomes of wheat and related chromosomes from a wild relative resulting in the generation of interspecific recombinant chromosomes. However, the ph1b mutant also enables recombination to occur between the homologous genomes of wheat, e.g. A/B, A/D, B/D, resulting in the generation of wheat intergenomic recombinant chromosomes. In this work we report on the presence of wheat intergenomic recombinants in the genomic background of hexaploid wheat/Amblyopyrum muticum introgression lines. The transfer of genomic rearrangements involving the D-genome through pentaploid crosses provides a strategy by which the D-genome of wheat can be introgressed into durum wheat. Hence, a pentaploid crossing strategy was used to transfer D-genome segments, introgressed with either the A- and/or the B-genome, into the tetraploid background of two durum wheat genotypes Karim and Om Rabi 5 in either the presence or absence of different Am. muticum (2n = 2x = 14, TT) introgressions. Introgressions were monitored in backcross generations to the durum wheat parents via multi-color genomic in situ hybridization (mc-GISH). Tetraploid lines carrying homozygous D-genome introgressions, as well as simultaneous homozygous D- and T-genome introgressions, were developed. Introgression lines were characterized via Kompetitive Allele-Specific PCR (KASP) markers and multi-color fluorescence in situ hybridization (FISH). Results showed that new wheat sub-genomic translocations were generated at each generation in progeny that carried any Am. muticum chromosome introgression irrespective of the linkage group that the segment was derived from. The highest frequencies of homologous recombination were observed between the A- and the D-genomes. Results indicated that the genotype Karim had a higher tolerance to genomic rearrangements and T-genome introgressions compared to Om Rabi 5. This indicates the importance of the selection of the parental genotype when attempting to transfer/develop introgressions into durum wheat from pentaploid crosses. CI - Copyright (c) 2019 Othmeni, Grewal, Hubbart-Edwards, Yang, Scholefield, Ashling, Yahyaoui, Gustafson, Singh, King and King. FAU - Othmeni, Manel AU - Othmeni M AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. FAU - Grewal, Surbhi AU - Grewal S AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. FAU - Hubbart-Edwards, Stella AU - Hubbart-Edwards S AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. FAU - Yang, Caiyun AU - Yang C AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. FAU - Scholefield, Duncan AU - Scholefield D AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. FAU - Ashling, Stephen AU - Ashling S AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. FAU - Yahyaoui, Amor AU - Yahyaoui A AD - International Maize and Wheat Improvement Center (CIMMYT) Mexico, Mexico City, Mexico. FAU - Gustafson, Perry AU - Gustafson P AD - Division of Plant Sciences, University of Missouri, Columbia, MO, United States. FAU - Singh, Pawan K AU - Singh PK AD - International Maize and Wheat Improvement Center (CIMMYT) Mexico, Mexico City, Mexico. FAU - King, Ian P AU - King IP AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. FAU - King, Julie AU - King J AD - Nottingham BBSRC Wheat Research Centre, Division of Plant and Cop Sciences, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire, United Kingdom. LA - eng PT - Journal Article DEP - 20190918 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC6760530 OTO - NOTNLM OT - Amblyopyrum muticum OT - Kompetitive Allele-Specific PCR markers OT - durum wheat OT - in situ hybridization OT - introgression OT - pentaploid crosses EDAT- 2019/10/18 06:00 MHDA- 2019/10/18 06:01 PMCR- 2019/01/01 CRDT- 2019/10/18 06:00 PHST- 2019/06/14 00:00 [received] PHST- 2019/08/13 00:00 [accepted] PHST- 2019/10/18 06:00 [entrez] PHST- 2019/10/18 06:00 [pubmed] PHST- 2019/10/18 06:01 [medline] PHST- 2019/01/01 00:00 [pmc-release] AID - 10.3389/fpls.2019.01110 [doi] PST - epublish SO - Front Plant Sci. 2019 Sep 18;10:1110. doi: 10.3389/fpls.2019.01110. eCollection 2019.