PMID- 31620995
OWN - NLM
STAT- MEDLINE
DCOM- 20200402
LR  - 20200402
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 190
IP  - 3
DP  - 2020 Mar
TI  - Development of a Novel Electroactive Cardiac Patch Based on Carbon Nanofibers and 
      Gelatin Encouraging Vascularization.
PG  - 931-948
LID - 10.1007/s12010-019-03135-6 [doi]
AB  - Tissue engineering makes it possible to fabricate scaffolds that can help the 
      function of defective tissues or even the most complex organs such as the heart. 
      Carbon nanofibers (CNFs), because of their high mechanical strength and 
      electrical properties, can improve the functional coupling of cardiomyocytes and 
      their electrophysiological properties. In this study, electroactive CNF/gelatin 
      (Gel) nanofibrous cardiac patches were prepared by an electrospinning method. 
      Scanning electron microscope (SEM) evaluation of prepared scaffolds showed 
      randomly oriented nanofibers. The electrical conductivity of the CNF/Gel 
      scaffolds was assessed by a four-probe device and was in the semiconducting range 
      (~ 10(-5) S/m). The result of an MTT assay confirmed the excellent 
      biocompatibility of electroactive CNF/Gel scaffolds. Also, CNF-containing 
      scaffolds supported cardiomyocyte adhesion and increased expression of the 
      cardiac genes including TrpT-2, Actn4, and Conx43 compared with the 
      non-conductive counterpart. Our findings also confirmed the angiogenic potential 
      of CNF/Gel scaffolds as compatible and electroactive platforms for cardiac tissue 
      engineering.
FAU - Mehrabi, Arezou
AU  - Mehrabi A
AD  - Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
FAU - Baheiraei, Nafiseh
AU  - Baheiraei N
AD  - Tissue Engineering and Applied Cell Sciences Division, Department of Hematology, 
      Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 
      n.baheiraei@modares.ac.ir.
FAU - Adabi, Mahdi
AU  - Adabi M
AD  - Department of Medical Nanotechnology, School of Advanced Technologies in 
      Medicine, Tehran University of Medical Sciences, Tehran, Iran.
FAU - Amirkhani, Zahra
AU  - Amirkhani Z
AD  - Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares 
      University, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20191016
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 7440-44-0 (Carbon)
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Carbon/*chemistry
MH  - Gelatin/*chemistry
MH  - *Heart
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Male
MH  - Mice
MH  - Myocytes, Cardiac/cytology
MH  - Nanofibers/*chemistry
MH  - *Neovascularization, Physiologic
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
OTO - NOTNLM
OT  - Carbon nanofiber
OT  - Cardiac patch
OT  - Electrospinning
OT  - Tissue engineering
OT  - Vascularization
EDAT- 2019/10/18 06:00
MHDA- 2020/04/03 06:00
CRDT- 2019/10/18 06:00
PHST- 2019/07/06 00:00 [received]
PHST- 2019/08/25 00:00 [accepted]
PHST- 2019/10/18 06:00 [pubmed]
PHST- 2020/04/03 06:00 [medline]
PHST- 2019/10/18 06:00 [entrez]
AID - 10.1007/s12010-019-03135-6 [pii]
AID - 10.1007/s12010-019-03135-6 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2020 Mar;190(3):931-948. doi: 
      10.1007/s12010-019-03135-6. Epub 2019 Oct 16.