PMID- 3162216
OWN - NLM
STAT- MEDLINE
DCOM- 19880411
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 229
IP  - 1
DP  - 1988 Feb 29
TI  - Inactivation of tissue inhibitor of metalloproteinases by neutrophil elastase and 
      other serine proteinases.
PG  - 157-60
AB  - Tissue inhibitor of metalloproteinases (TIMP) from cultured bovine dental pulp 
      inhibits human rheumatoid synovial matrix metalloproteinase 3 (MMP-3) with a 
      stoichiometry of 1:1 on a molar basis. Among the serine proteinases examined, 
      human neutrophil elastase, trypsin and alpha-chymotrypsin destroyed the 
      inhibitory activity of TIMP against MMP-3 by degrading the inhibitor molecule 
      into small fragments. In contrast, the inhibitory activity of TIMP was not 
      significantly reduced by the actions of cathepsin G, pancreatic elastase and 
      plasmin. These data indicate that neutrophils which infiltrate tissues in various 
      inflammatory conditions may play an important role in regulating TIMP activity in 
      vivo through the action of neutrophil elastase.
FAU - Okada, Y
AU  - Okada Y
AD  - Department of Pathology, School of Medicine, Kanazawa University, Japan.
FAU - Watanabe, S
AU  - Watanabe S
FAU - Nakanishi, I
AU  - Nakanishi I
FAU - Kishi, J
AU  - Kishi J
FAU - Hayakawa, T
AU  - Hayakawa T
FAU - Watorek, W
AU  - Watorek W
FAU - Travis, J
AU  - Travis J
FAU - Nagase, H
AU  - Nagase H
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.1 (Chymotrypsin)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
RN  - EC 3.4.21.4 (Trypsin)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
MH  - Animals
MH  - Arthritis, Rheumatoid/enzymology
MH  - Cattle
MH  - Chymotrypsin/metabolism
MH  - Enzyme Inhibitors/*pharmacology
MH  - Humans
MH  - Metalloendopeptidases/*antagonists & inhibitors
MH  - Neutrophils/*enzymology
MH  - Pancreatic Elastase/*blood
MH  - Serine Endopeptidases/*blood
MH  - Synovial Membrane/enzymology
MH  - Tissue Inhibitor of Metalloproteinases
MH  - Trypsin/metabolism
EDAT- 1988/02/29 00:00
MHDA- 1988/02/29 00:01
CRDT- 1988/02/29 00:00
PHST- 1988/02/29 00:00 [pubmed]
PHST- 1988/02/29 00:01 [medline]
PHST- 1988/02/29 00:00 [entrez]
AID - 10.1016/0014-5793(88)80817-2 [doi]
PST - ppublish
SO  - FEBS Lett. 1988 Feb 29;229(1):157-60. doi: 10.1016/0014-5793(88)80817-2.