PMID- 31622607
OWN - NLM
STAT- MEDLINE
DCOM- 20191217
LR  - 20191217
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 238
DP  - 2019 Dec 1
TI  - Rheumatoid arthritis induces enteric neurodegeneration and jejunal inflammation, 
      and quercetin promotes neuroprotective and anti-inflammatory actions.
PG  - 116956
LID - S0024-3205(19)30883-5 [pii]
LID - 10.1016/j.lfs.2019.116956 [doi]
AB  - AIMS: The aim of our study was to study the pathological mechanisms induced by 
      the rheumatoid arthritis (RA) on the Enteric Nervous System (ENS). MAIN METHODS: 
      We evaluated the effect of the chronic arthritis and its treatment with 50 mg/kg 
      quercetin alone (AQ) and combined with 17.5 mg/kg ibuprofen (AIQ) for 60 days on 
      neurons, glial cells and intestinal wall. Other groups were used: control (C), 
      arthritic (A) and arthritic treated with 17.5 mg/kg ibuprofen (AI). After 60 
      days, the jejunum was removed and processed for immunohistochemical techniques. 
      Immunostainings were performed for HuC/D and S100 (myenteric and submucosal 
      plexuses), and GFAP (only myenteric plexus), while immunolabeling for CD45 and 
      CD20 lymphocytes was performed using cryosections. Western blot was performed for 
      GDNF, S100 and GFAP. KEY FINDINGS: A group yielded a remarkable density decrease 
      of the neurons and glial cells with morphometric changes in the myenteric and 
      submucosal plexuses, reduction of the GDNF expression and GFAP-related parameters 
      (GFAP expression, occupancy area and GFAP-expressing glial cells) and intestinal 
      inflammation and atrophy of the mucosa and intestinal wall. AQ group 
      substantially reversed most of these effects, except for intestinal atrophy of 
      the jejunum. The AI and AIQ groups displayed lower beneficial results than AQ for 
      parameters related to the neurons and glial cells, although AIQ did not prevent 
      the inflammation of the mucosa. SIGNIFICANCE: The severe chronic rheumatoid 
      arthritis induced severe effects on ENS and mucosa, and quercetin treatment 
      continues to be an important antioxidant supplement preventing the progression of 
      the RA severity.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Piovezana Bossolani, Gleison Daion
AU  - Piovezana Bossolani GD
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil.
FAU - Silva, Bruna Thais
AU  - Silva BT
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil.
FAU - Colombo Martins Perles, Juliana Vanessa
AU  - Colombo Martins Perles JV
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil.
FAU - Lima, Mariana Machado
AU  - Lima MM
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil.
FAU - Vieira Frez, Flavia Cristina
AU  - Vieira Frez FC
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil.
FAU - Garcia de Souza, Sara Raquel
AU  - Garcia de Souza SR
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil.
FAU - Sehaber-Sierakowski, Camila Caviquioli
AU  - Sehaber-Sierakowski CC
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil.
FAU - Bersani-Amado, Ciomar Aparecida
AU  - Bersani-Amado CA
AD  - Department of Pharmacology and Therapeutic, Universidade Estadual de Maringa, 
      Maringa, Parana, Brazil.
FAU - Zanoni, Jacqueline Nelisis
AU  - Zanoni JN
AD  - Department of Morphological Sciences, Universidade Estadual de Maringa, 
      87020-900, Maringa, Parana, Brazil. Electronic address: jnzanoni@uem.br.
LA  - eng
PT  - Journal Article
DEP - 20191014
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Neuroprotective Agents)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/pharmacology
MH  - Arthritis, Experimental/chemically induced/*complications
MH  - Arthritis, Rheumatoid/*complications
MH  - Enteric Nervous System/drug effects/pathology
MH  - Inflammation/*drug therapy/etiology/pathology
MH  - Jejunum/*drug effects/immunology/pathology
MH  - Male
MH  - Neurodegenerative Diseases/*drug therapy/etiology/pathology
MH  - Neuroprotection/drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - Quercetin/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - Enteric nervous system
OT  - Glia
OT  - Quercetin
OT  - Rheumatoid arthritis
EDAT- 2019/10/18 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/10/18 06:00
PHST- 2019/08/21 00:00 [received]
PHST- 2019/10/08 00:00 [revised]
PHST- 2019/10/10 00:00 [accepted]
PHST- 2019/10/18 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/10/18 06:00 [entrez]
AID - S0024-3205(19)30883-5 [pii]
AID - 10.1016/j.lfs.2019.116956 [doi]
PST - ppublish
SO  - Life Sci. 2019 Dec 1;238:116956. doi: 10.1016/j.lfs.2019.116956. Epub 2019 Oct 
      14.