PMID- 31622806
OWN - NLM
STAT- MEDLINE
DCOM- 20191211
LR  - 20191217
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 305
DP  - 2020 Feb 1
TI  - Effect of EDTA enriched diets on farmed fish allergenicity and muscle quality; a 
      proteomics approach.
PG  - 125508
LID - S0308-8146(19)31626-7 [pii]
LID - 10.1016/j.foodchem.2019.125508 [doi]
AB  - Fish is one of the most common elicitors of food-allergic reactions worldwide. 
      These reactions are triggered by the calcium-binding muscle protein 
      beta-parvalbumin, which was shown to have reduced immunoglobulin E (IgE)-binding 
      capacity upon calcium depletion. This work aimed to reduce gilthead seabream 
      allergenicity using diets supplemented with a calcium chelator. Three 
      experimental feeds were tested, differing in ethylenediaminetetraacetic acid 
      (EDTA) supplementation, and its effects on muscle and parvalbumin's 
      IgE-reactivity were analyzed. Chromatographic determination of EDTA showed no 
      accumulation in the muscle and sensory results demonstrated that the lowest 
      concentration did not affect fish quality as edible fish. Proteomics revealed one 
      protein related to muscle contraction with significantly different relative 
      abundance. Immunoblot assays performed with fish-allergic patients sera indicated 
      a 50% reduction in IgE-reactivity upon EDTA presence. These preliminary results 
      provide the basis for the further development of a non-GMO approach to modulate 
      fish allergenicity and improve safety of aquaculture fish.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - De Magalhaes, Claudia Raposo
AU  - De Magalhaes CR
AD  - CCMAR, Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal. 
      Electronic address: csraposo@ualg.pt.
FAU - Schrama, Denise
AU  - Schrama D
AD  - CCMAR, Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal. 
      Electronic address: dschrama@ualg.pt.
FAU - Fonseca, Flavio
AU  - Fonseca F
AD  - Instituto Federal de Educacao, Ciencia e Tecnologia do Amazonas - Campus Zona 
      Leste, Av. Cosme Ferreira, 8045, Bairro Gilberto Mestrinho, 69086-475 Manaus, AM, 
      Brazil. Electronic address: flavio.fonseca@ifam.edu.br.
FAU - Kuehn, Annette
AU  - Kuehn A
AD  - Luxembourg Institute of Health, Department of Infection and Immunity, 29, Rue 
      Henri Koch, L-4354 Esch-sur-Alzette, Luxembourg. Electronic address: 
      Annette.kuehn@lih.lu.
FAU - Morisset, Martine
AU  - Morisset M
AD  - National Unit of Immunology and Allergology, Centre Hospitalier de Luxembourg, 
      Luxembourg; Allergy Unit, Angers University Hospital, 4 Rue Larrey, 49993 Angers, 
      France. Electronic address: Martine.Morisset@chu-angers.fr.
FAU - Ferreira, Sara R
AU  - Ferreira SR
AD  - CCMAR, Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal. 
      Electronic address: srferreira@ualg.pt.
FAU - Goncalves, Amparo
AU  - Goncalves A
AD  - IPMA I.P., Portuguese Institute for the Sea and Atmosphere, Division of 
      Aquaculture and Upgrading, Rua Alfredo Magalhaes Ramalho, 6, 1495-006 Lisboa, 
      Portugal. Electronic address: amparo@ipma.pt.
FAU - Rodrigues, Pedro M
AU  - Rodrigues PM
AD  - CCMAR, Centre of Marine Sciences, University of Algarve, 8005-139 Faro, Portugal. 
      Electronic address: pmrodrig@ualg.pt.
LA  - eng
PT  - Journal Article
DEP - 20190911
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Parvalbumins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Animals
MH  - *Aquaculture
MH  - Edetic Acid/*analysis
MH  - Fishes/*immunology/metabolism
MH  - *Food Quality
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Muscles/*chemistry
MH  - Parvalbumins/immunology
MH  - *Proteomics
MH  - Seafood
OTO - NOTNLM
OT  - Ethylenediaminetetraacetic acid (PubChem CID: 6049)
OT  - Fish allergens
OT  - Fish nutrition
OT  - Gilthead seabream
OT  - IgE-reactivity
OT  - Parvalbumin
EDAT- 2019/10/18 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/10/18 06:00
PHST- 2019/05/07 00:00 [received]
PHST- 2019/09/05 00:00 [revised]
PHST- 2019/09/09 00:00 [accepted]
PHST- 2019/10/18 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/10/18 06:00 [entrez]
AID - S0308-8146(19)31626-7 [pii]
AID - 10.1016/j.foodchem.2019.125508 [doi]
PST - ppublish
SO  - Food Chem. 2020 Feb 1;305:125508. doi: 10.1016/j.foodchem.2019.125508. Epub 2019 
      Sep 11.