PMID- 31626878
OWN - NLM
STAT- MEDLINE
DCOM- 20210104
LR  - 20210104
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 714
DP  - 2020 Jan 1
TI  - Suppression of miR-10a-5p in bone marrow mesenchymal stem cells enhances the 
      therapeutic effect on spinal cord injury via BDNF.
PG  - 134562
LID - S0304-3940(19)30665-2 [pii]
LID - 10.1016/j.neulet.2019.134562 [doi]
AB  - BACKGROUNDS/AIMS: Brain-derived neurotrophic factor (BDNF) plays a primary role 
      in the maturation, proliferation, and differentiation of neuronal cells, can 
      induce bone-marrow-derived mesenchymal stem cells (MSCs) to differentiate into 
      nerve cells. This study aims to explore whether regulation of BDNF through 
      microRNAs (miRNAs) in MSCs may further enhance the therapeutic effect on spinal 
      cord injury (SCI). METHODS: Bioinformatics analyses were done to predict miRNAs 
      that target BDNF in MSCs. Dual-luciferase reporter gene assays were performed to 
      verify the target relationship between microRNA and BDNF. We examined the mRNA 
      and protein levels of BDNF in MSCs by RT-qPCR and Western blot, respectively. CCK 
      8 assay was chosen to assess cell viability. MSCs were transduced with 
      miR-10a-5p-ASO, which were transplanted into rats that underwent SCI. The tissue 
      integrity percentage, cavity volume, and Basso-Beattie-Bresnahan (BBB) scale were 
      assessed. Neurofilament (NF) was detected using immunohistochemistry. 
      Histological features of spinal cord tissues examined following HE staining. 
      RESULTS: MiR-10a-5p inhibited protein translation of BDNF, through binding to the 
      3'-UTR of the BDNF. MSCs transduced with MiR-10a-5p-ASO further increased the 
      tissue integrity percentage, decreased cavity volume, and enhanced the recovery 
      of BBB score in SCI model rats, compared to control MSCs. CONCLUSION: 
      Upregulation of BDNF by miR-10a-5p suppression in MSCs further improve the 
      therapeutic potential of MSCs in treating SCI in rats.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - Zhang, Tongxia
AU  - Zhang T
AD  - Department of Neurology, Research Institute of Neuromuscular and 
      Neurodegenerative Diseases, Qilu Hospital of Shandong University, Jinan, 250012, 
      China.
FAU - Liu, Chong
AU  - Liu C
AD  - Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of 
      Brain and Brain-Inspired Science, Shandong University, Jinan, 250012, China.
FAU - Chi, Lingyi
AU  - Chi L
AD  - Department of Neurosurgery, Qilu Hospital of Shandong University and Institute of 
      Brain and Brain-Inspired Science, Shandong University, Jinan, 250012, China. 
      Electronic address: chilingyi@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191015
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN10 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Oligonucleotides, Antisense)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Bone Marrow Cells
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cell Differentiation
MH  - Cell Proliferation/drug effects/*genetics
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/drug effects/*metabolism
MH  - MicroRNAs/antagonists & inhibitors/*metabolism
MH  - Neurogenesis/drug effects/*genetics
MH  - Oligonucleotides, Antisense/pharmacology
MH  - Protein Biosynthesis
MH  - Rats
MH  - Spinal Cord Injuries/metabolism/pathology/*therapy
MH  - Up-Regulation
OTO - NOTNLM
OT  - BDNF
OT  - Bone marrow mesenchymal stem cells
OT  - Spinal cord injury
OT  - miR-10a-5p
EDAT- 2019/10/19 06:00
MHDA- 2021/01/05 06:00
CRDT- 2019/10/19 06:00
PHST- 2019/06/20 00:00 [received]
PHST- 2019/09/28 00:00 [revised]
PHST- 2019/10/14 00:00 [accepted]
PHST- 2019/10/19 06:00 [pubmed]
PHST- 2021/01/05 06:00 [medline]
PHST- 2019/10/19 06:00 [entrez]
AID - S0304-3940(19)30665-2 [pii]
AID - 10.1016/j.neulet.2019.134562 [doi]
PST - ppublish
SO  - Neurosci Lett. 2020 Jan 1;714:134562. doi: 10.1016/j.neulet.2019.134562. Epub 
      2019 Oct 15.