PMID- 31627364
OWN - NLM
STAT- MEDLINE
DCOM- 20201016
LR  - 20211204
IS  - 2072-6651 (Electronic)
IS  - 2072-6651 (Linking)
VI  - 11
IP  - 10
DP  - 2019 Oct 17
TI  - Carboxyl-Functionalized, Europium Nanoparticle-Based Fluorescent 
      Immunochromatographic Assay for Sensitive Detection of Citrinin in Monascus 
      Fermented Food.
LID - 10.3390/toxins11100605 [doi]
LID - 605
AB  - A fluorescent immunochromatographic test strip (FICTS) based on the use of 
      europium nanoparticles (EuNPs) was developed and applied to detect citrinin (CIT) 
      in Monascus fermented food. The sensitivity of the immunoassay to detect CIT was 
      greatly improved by the use of a specific monoclonal antibody to attach EuNPs to 
      form a probe. Under optimum conditions, the visual detection limit was 2.5 ng/mL, 
      and the detection limit of the instrument was 0.05 ng/mL. According to the 
      results, the IC(50) was 0.4 ng/mL. Matrix interference from various Monascus 
      fermented foods was investigated in food sample detection. The immunosensor also 
      demonstrated high recoveries (86.8-113.0%) and low relative standard deviations 
      (RSDs) (1.8-15.3%) when testing spiked Monascus fermented food. The detection 
      results of this method showed a good correlation (R(2) > 0.98) with 
      high-performance liquid chromatography (HPLC). The results showed that the FICTS 
      method could be used as a rapid, sensitive method to detect CIT in Monascus 
      fermented food.
FAU - Chen, Erjing
AU  - Chen E
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou 310018, China. s1709071002@cjlu.edu.cn.
FAU - Xu, Ying
AU  - Xu Y
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou 310018, China. s1809071028@cjlu.edu.cn.
FAU - Ma, Biao
AU  - Ma B
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou 310018, China. 16a0701109@cjlu.edu.cn.
FAU - Cui, Haifeng
AU  - Cui H
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou 310018, China. hfcui@cjlu.edu.cn.
FAU - Sun, Chuanxin
AU  - Sun C
AD  - Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, 
      Swedish University of Agricultural Science (SLU), P.O. Box 7080, SE-75007 
      Uppsala, Sweden. Chuanxin.Sun@slu.se.
FAU - Zhang, Mingzhou
AU  - Zhang M
AUID- ORCID: 0000-0002-8203-8090
AD  - Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, 
      China Jiliang University, Hangzhou 310018, China. zmzcjlu@cjlu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191017
PL  - Switzerland
TA  - Toxins (Basel)
JT  - Toxins
JID - 101530765
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 3S697X6SNZ (Citrinin)
RN  - 444W947O8O (Europium)
RN  - 7440-57-5 (Gold)
RN  - ZIF514RVZR (Serum Albumin, Human)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry
MH  - Antigens/chemistry
MH  - Citrinin/*analysis/chemistry
MH  - Europium/*chemistry
MH  - Female
MH  - Fermented Foods/*analysis
MH  - Fluorescence
MH  - Food Contamination/analysis
MH  - Gold/chemistry
MH  - Immunoassay
MH  - Metal Nanoparticles/*chemistry
MH  - Mice, Inbred BALB C
MH  - Monascus/*chemistry
MH  - Serum Albumin, Bovine/chemistry
MH  - Serum Albumin, Human/chemistry
PMC - PMC6832703
OTO - NOTNLM
OT  - Citrinin
OT  - Europium nanoparticles
OT  - Fluorescent immunochromatographic assay
OT  - Monascus fermented food
COIS- The authors declare no competing financial interest.
EDAT- 2019/10/20 06:00
MHDA- 2020/10/21 06:00
PMCR- 2019/10/01
CRDT- 2019/10/20 06:00
PHST- 2019/08/25 00:00 [received]
PHST- 2019/10/03 00:00 [revised]
PHST- 2019/10/15 00:00 [accepted]
PHST- 2019/10/20 06:00 [entrez]
PHST- 2019/10/20 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/10/01 00:00 [pmc-release]
AID - toxins11100605 [pii]
AID - toxins-11-00605 [pii]
AID - 10.3390/toxins11100605 [doi]
PST - epublish
SO  - Toxins (Basel). 2019 Oct 17;11(10):605. doi: 10.3390/toxins11100605.