PMID- 31629034 OWN - NLM STAT- MEDLINE DCOM- 20210104 LR - 20210104 IS - 1872-7972 (Electronic) IS - 0304-3940 (Linking) VI - 714 DP - 2020 Jan 1 TI - Low-dose alcohol ameliorated homocysteine-induced anxiety-related behavior via attenuating oxidative stress in mice. PG - 134568 LID - S0304-3940(19)30671-8 [pii] LID - 10.1016/j.neulet.2019.134568 [doi] AB - Recent studies showed that homocysteine (Hcy) levels were obviously elevated in patients with anxiety, furthermore, oxidative stress and inflammation were closely linked with Hcy-related damage. Despite alcohol exposure has differential effects on different forms of anxiety, the role of alcohol on anxiety-related behavior induced by high Hcy levels is still not entirely clear. The present study investigated the protective potential of low-dose alcohol against homocysteine-induced anxiety-related behavior and explored the possible underlying mechanisms. Mice were administered intragastrically with methionine (2.0 g/kg/day) or alcohol (0.6 g/kg/day). After 21 days of administration, the anxiety-related behavior was evaluated through open field (OF) and elevated plus maze (EPM) tests, and the variations of oxidative stress and inflammation levels were measured. The results of OF and EPM tests showed that the anxiety-related behavior in mice was prevented by alcohol treatment. Alcohol lowered the elevated serum Hcy levels and alleviated the damage of hippocampal tissues in hyperhomocysteinemia (HHcy) mice. Meanwhile, the superoxide dismutase (SOD) activity of the hippocampal tissues enhanced, and the malondialdehyde (MDA) concentration of the hippocampal tissues and the serum interleukin-1beta (IL-1beta) level decreased. In addition, after administering alcohol, the increase of superoxide dismutase 1 (SOD1), heme oxygenase 1 (HO-1) protein expression and the decrease of IL-1beta protein expression were also detected in HHcy mice hippocampal tissues. Taken together, low-dose alcohol significantly ameliorated the Hcy-induced anxiety-related behavior in mice, which might be related to SOD1 and HO-1 upregulation and IL-1beta downregulation. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Cheng, Jie AU - Cheng J AD - Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. FAU - Li, Fan AU - Li F AD - Basic Medical Experiment Teaching Center, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. FAU - Sun, Xiaoming AU - Sun X AD - Basic Medical Experiment Teaching Center, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. FAU - Liu, Shuqin AU - Liu S AD - Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, Shaanxi, 710061, China. FAU - Chen, Lina AU - Chen L AD - Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, Shaanxi, 710061, China. FAU - Tian, Feng AU - Tian F AD - Grade 2015, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. FAU - Zhao, Zhenghang AU - Zhao Z AD - Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, Shaanxi, 710061, China. FAU - Hu, Hao AU - Hu H AD - Department of Pharmacology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China; Basic Medical Experiment Teaching Center, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University, Ministry of Education of China, Xi'an, Shaanxi, 710061, China. Electronic address: huhao@mail.xjtu.edu.cn. FAU - Li, Xiaogang AU - Li X AD - Department of Anesthesiology, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. Electronic address: lxg945945@sina.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191016 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Central Nervous System Depressants) RN - 0 (IL1B protein, mouse) RN - 0 (Interleukin-1beta) RN - 0 (Membrane Proteins) RN - 0LVT1QZ0BA (Homocysteine) RN - 3K9958V90M (Ethanol) RN - EC 1.14.14.18 (Heme Oxygenase-1) RN - EC 1.14.14.18 (Hmox1 protein, mouse) RN - EC 1.15.1.1 (Sod1 protein, mouse) RN - EC 1.15.1.1 (Superoxide Dismutase-1) SB - IM MH - Animals MH - Anxiety/*metabolism/psychology MH - Behavior, Animal/*drug effects MH - Central Nervous System Depressants/*pharmacology MH - Elevated Plus Maze Test MH - Ethanol/*pharmacology MH - Heme Oxygenase-1/drug effects/metabolism MH - Hippocampus/*drug effects/metabolism MH - Homocysteine MH - Hyperhomocysteinemia/*metabolism/psychology MH - Inflammation MH - Interleukin-1beta/drug effects/metabolism MH - Male MH - Membrane Proteins/drug effects/metabolism MH - Mice MH - Open Field Test MH - Oxidative Stress/*drug effects MH - Superoxide Dismutase-1/drug effects/metabolism OTO - NOTNLM OT - Alcohol OT - Anxiety-related behavior OT - Homocysteine OT - Inflammation OT - Oxidative stress EDAT- 2019/10/20 06:00 MHDA- 2021/01/05 06:00 CRDT- 2019/10/20 06:00 PHST- 2019/08/13 00:00 [received] PHST- 2019/10/08 00:00 [revised] PHST- 2019/10/15 00:00 [accepted] PHST- 2019/10/20 06:00 [pubmed] PHST- 2021/01/05 06:00 [medline] PHST- 2019/10/20 06:00 [entrez] AID - S0304-3940(19)30671-8 [pii] AID - 10.1016/j.neulet.2019.134568 [doi] PST - ppublish SO - Neurosci Lett. 2020 Jan 1;714:134568. doi: 10.1016/j.neulet.2019.134568. Epub 2019 Oct 16.