PMID- 31629090
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-1597 (Electronic)
IS  - 1572-1000 (Linking)
VI  - 33
DP  - 2021 Mar
TI  - Significance of in vitro photodynamic cytodiagnosis using 5-aminolevulinic acid 
      in solid pancreatic tumors extracted by endoscopic ultrasound-guided fine-needle 
      aspiration.
PG  - 101581
LID - S1572-1000(19)30432-6 [pii]
LID - 10.1016/j.pdpdt.2019.101581 [doi]
AB  - BACKGROUND: Recently, photodynamic diagnosis using 5-aminolevulinic acid (5-ALA) 
      has gained attention for the diagnosis of neoplastic diseases. In the present 
      study, an in vitro method of photodynamic cytodiagnosis (PDCD) performed using 
      the reagent 5-ALA in the cytodiagnosis of solid pancreatic tumors was developed. 
      Here, we assess the accuracy of PDCD for malignancy. MATERIALS AND METHODS: 
      EUS-FNA was performed from September 2015 to March 2018 in patients with solid 
      pancreatic tumors at Osaka Rosai Hospital. Samples were diagnosed independently 
      by an expert pathologist and a medical doctor with conventional cytology and 
      PDCD. RESULTS: A total of 53 patients (35 males, average age: 70.2 years old) 
      were enrolled. The definitive diagnoses were 7 benign lesions and 46 malignant 
      lesions. Using the in vitro PDCD method, the detection of reddish fluorescence in 
      cell samples indicated cancer cells. PDCD had a sensitivity of 91.3% (42/46) and 
      a specificity of 100% (7/7), while conventional cytology had a sensitivity of 
      93.5% (43/46) and a specificity of 85.7% (6/7). Two patients were successfully 
      diagnosed with malignancy only by the PDCD method. CONCLUSIONS: In vitro PDCD 
      performed using the 5-ALA method can effectively and safely identify a diagnosis 
      of pancreatic cancer without requiring an expert pathologist. The sensitivity of 
      this technique could be increased in the diagnosis of pancreatic malignancy by 
      combining it with the conventional method.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - Hirao, Motohiro
AU  - Hirao M
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan. 
      Electronic address: snowmotty@yahoo.co.jp.
FAU - Hosui, Atsushi
AU  - Hosui A
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Mimura, Akihiro
AU  - Mimura A
AD  - Department of Pathology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Tanimoto, Takafumi
AU  - Tanimoto T
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Ohnishi, Kosaku
AU  - Ohnishi K
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Kusumoto, Yukihiro
AU  - Kusumoto Y
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Yamaguchi, Toshio
AU  - Yamaguchi T
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Yamada, Takuya
AU  - Yamada T
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Miwa, Hideaki
AU  - Miwa H
AD  - Department of Pathology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
FAU - Hiramatsu, Naoki
AU  - Hiramatsu N
AD  - Department of Gastroenterology, Osaka Rosai Hospital, Sakai, Osaka, Japan.
LA  - eng
PT  - Journal Article
DEP - 20191016
PL  - Netherlands
TA  - Photodiagnosis Photodyn Ther
JT  - Photodiagnosis and photodynamic therapy
JID - 101226123
RN  - 0 (Photosensitizing Agents)
RN  - 88755TAZ87 (Aminolevulinic Acid)
SB  - IM
MH  - Aged
MH  - Aminolevulinic Acid
MH  - Endoscopic Ultrasound-Guided Fine Needle Aspiration
MH  - Humans
MH  - Male
MH  - *Pancreatic Neoplasms/diagnostic imaging
MH  - *Photochemotherapy/methods
MH  - Photosensitizing Agents
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Aminolevulinic acid
OT  - EUS
OT  - Pancreatic adenocarcinoma
OT  - Pancreatic tumor
OT  - Photodynamic diagnosis
EDAT- 2019/10/20 06:00
MHDA- 2021/05/15 06:00
CRDT- 2019/10/20 06:00
PHST- 2019/08/28 00:00 [received]
PHST- 2019/10/15 00:00 [accepted]
PHST- 2019/10/20 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2019/10/20 06:00 [entrez]
AID - S1572-1000(19)30432-6 [pii]
AID - 10.1016/j.pdpdt.2019.101581 [doi]
PST - ppublish
SO  - Photodiagnosis Photodyn Ther. 2021 Mar;33:101581. doi: 
      10.1016/j.pdpdt.2019.101581. Epub 2019 Oct 16.