PMID- 31629408 OWN - NLM STAT- MEDLINE DCOM- 20200330 LR - 20200330 IS - 1757-2215 (Electronic) IS - 1757-2215 (Linking) VI - 12 IP - 1 DP - 2019 Oct 19 TI - Intercellular adhesion molecule-1 expression and serum levels as markers of pre-clinical atherosclerosis in polycystic ovary syndrome. PG - 97 LID - 10.1186/s13048-019-0566-5 [doi] LID - 97 AB - BACKGROUND: Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disorder characterized by obesity, hyperandrogenism, and insulin resistance. Intercellular adhesion molecule-1 (ICAM-1) is a proinflammatory and proatherogenic cytokine which is associated with atherosclerosis, insulin resistance, and cardiovascular disease (CVD). The pathogenesis of PCOS is not precisely known. Thus, the purpose of this study was to investigate the potential role of ICAM-1 expression and serum ICAM-1 concentrations in pathogenesis of PCOS. Moreover, we aimed to evaluate the possible relationship between ICAM-1 gene expression with carotid intima-media thickness as well as clinic-morphological features of PCOS. METHODS: This case control study enrolled 180 patients with PCOS and 120 controls groups and they were stratified according to their fasting plasma glucose (FPG) into three subgroups; normal glucose tolerance (NGT) [n = 75], those with impaired glucose tolerance (IGT) [n = 65], and 40 patients with type 2 diabetes mellitus (T2DM). Circulating ICAM-1 expression levels were determined by real time polymerase chain reaction (RT-PCR). Serum ICAM-1 concentrations were measured using enzyme-linked immunosorbent assay (ELISA). RESULTS: Our results revealed that PCOS patients had higher values of ICAM-1expression and serum levels. Among PCOS patients, T2DM patients had the highest values of ICAM-1 expression and serum levels compared to IGT and NGT subgroups. The ICAM-1 expression and serum levels were significantly positive correlated with cardiovascular risk and PCOS phenotypes. Linear regression test showed that HOMA-IR was the main predictors of serum ICAM-1 levels in PCOS. Receiver operating characteristic curve (ROC) analysis revealed that, the power of ICAM-1 expression levels was higher than serum ICAM-1 in diagnosis of PCOS and in differentiating T2DM from IGT and NGT subgroups. Interestingly, combination of both ICAM-1 expression and serum levels improved the diagnostic role of serum ICAM-1. CONCLUSION: ICAM-1 expression and serum levels were higher in women with PCOS compared to control group also, there was a strong independent association between higher ICAM-1 expression and serum levels with cardiovascular risks in PCOS group. FAU - Rashad, Nearmeen M AU - Rashad NM AUID- ORCID: 0000-0002-8587-0616 AD - Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. nrashad78@yahoo.com. FAU - El-Shal, Amal S AU - El-Shal AS AD - Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Abomandour, Hala G AU - Abomandour HG AD - Cardiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Aboelfath, Amr Mostafa Kamel AU - Aboelfath AMK AD - Obstetrics and Gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Rafeek, Mohamed El Sayed AU - Rafeek MES AD - Obstetrics and Gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Badr, Mohammad Samir AU - Badr MS AD - Obstetrics and Gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Ali, Ayman E AU - Ali AE AD - Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Yousef, Mohammed S AU - Yousef MS AD - Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Fathy, Maha Abdelhamid AU - Fathy MA AD - Physiology Department, Faculty of Medicine-Zagazig University, Zagazig, Egypt. FAU - Sharaf El Din, Mustafa Taha Abdelfattah AU - Sharaf El Din MTA AD - Obstetrics and Gynecology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. LA - eng PT - Journal Article DEP - 20191019 PL - England TA - J Ovarian Res JT - Journal of ovarian research JID - 101474849 RN - 0 (Biomarkers) RN - 0 (ICAM1 protein, human) RN - 0 (RNA, Messenger) RN - 126547-89-5 (Intercellular Adhesion Molecule-1) SB - IM MH - Adult MH - Atherosclerosis/*etiology/pathology MH - Biomarkers MH - Case-Control Studies MH - Female MH - *Gene Expression MH - Humans MH - Intercellular Adhesion Molecule-1/*blood/*genetics MH - Middle Aged MH - Polycystic Ovary Syndrome/blood/complications/*genetics MH - RNA, Messenger/genetics MH - ROC Curve MH - Young Adult PMC - PMC6800507 OTO - NOTNLM OT - Cardiovascular OT - Enzyme-linked immunosorbent assay OT - Intercellular adhesion molecule-1 OT - Polycystic ovary syndrome OT - Type 2 diabetes mellitus COIS- The authors declare that they have no competing interests. EDAT- 2019/10/21 06:00 MHDA- 2020/03/31 06:00 PMCR- 2019/10/19 CRDT- 2019/10/21 06:00 PHST- 2019/05/28 00:00 [received] PHST- 2019/09/10 00:00 [accepted] PHST- 2019/10/21 06:00 [entrez] PHST- 2019/10/21 06:00 [pubmed] PHST- 2020/03/31 06:00 [medline] PHST- 2019/10/19 00:00 [pmc-release] AID - 10.1186/s13048-019-0566-5 [pii] AID - 566 [pii] AID - 10.1186/s13048-019-0566-5 [doi] PST - epublish SO - J Ovarian Res. 2019 Oct 19;12(1):97. doi: 10.1186/s13048-019-0566-5.