PMID- 31629858 OWN - NLM STAT- MEDLINE DCOM- 20200721 LR - 20200721 IS - 1090-2430 (Electronic) IS - 0014-4886 (Linking) VI - 323 DP - 2020 Jan TI - Acrolein is involved in ischemic stroke-induced neurotoxicity through spermidine/spermine-N1-acetyltransferase activation. PG - 113066 LID - S0014-4886(19)30215-8 [pii] LID - 10.1016/j.expneurol.2019.113066 [doi] AB - BACKGROUND AND PURPOSE: Ischemic stroke is the most common type of cerebrovascular event and is responsible for approximately 85% of all strokes in Taiwan. Neurons contain high concentrations of polyamines, which are prone to various pathological states in the brain and are perturbed after cerebral ischemia. Acrolein, an alpha,beta-unsaturated aldehyde, has been suggested as the primary culprit of neuronal damage in stroke patients. However, the mechanism by which acrolein induces neuronal damage during ischemic stroke is not clear. METHODS: Urinary 3-hydroxypropyl mercapturic acid (3-HPMA), an acrolein-glutathione (GSH) metabolite, plasma acrolein-protein conjugates (Acr-PC) and plasma GSH levels were analyzed to correlate disease severity and prognosis of stroke patients compared with control subjects. In vivo middle cerebral artery occlusion (MCAO) animal models and an in vitro oxygen glucose deprivation (OGD) stroke model were used to investigate the mechanisms of acrolein-induced neuronal damage. RESULTS: A deregulated acrolein metabolism, including significantly increased plasma Acr-PC levels, decreased urinary 3-HPMA levels and decreased plasma GSH levels, was found in stroke patients compared to control subjects. We further observed that acrolein was produced during ischemia resulting in brain damage in in vivo MCAO animal model. The induction of acrolein in neuronal cells during OGD occurred due to the increased expression of spermidine/spermine N1-acetyltransferase (SSAT) by NF-kB pathway activation. In addition, acrolein elicited a vicious cycling of oxidative stress resulting in neurotoxicity. Finally, N-acetylcysteine effectively prevented OGD-induced neurotoxicity by scavenging acrolein. CONCLUSION: Overall, our current results demonstrate that acrolein is a culprit of neuronal damage through GSH depletion in stroke patients. The mechanism underlying the role of acrolein in stroke-related neuronal damage occurs through SSAT-induced polyamine oxidation by NF-kB pathway activation. These results provide a novel mechanism of neurotoxicity in stroke patients, aid in the development of neutralizing or preventive measures, and further our understanding of neural protection. CI - Copyright (c) 2019 Elsevier Inc. All rights reserved. FAU - Liu, Jin-Hui AU - Liu JH AD - Institute of Food Safety and Health Risk Assessment, National Yang-Ming UniversRity, Taipei, Taiwan. FAU - Wang, Tse-Wen AU - Wang TW AD - Institute of Food Safety and Health Risk Assessment, National Yang-Ming UniversRity, Taipei, Taiwan. FAU - Lin, Yung-Yang AU - Lin YY AD - Department of Neurology, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan; Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Division of Cerebrovascular Diseases, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan; Institute of Brain Science, National Yang-Ming University, Taipei, Taiwan; Department of CritiWcal Care Medicine, Taipei Veterans General Hospital, Taipei, Taiwan. FAU - Ho, Wen-Chien AU - Ho WC AD - Department of Pharmacology, National Yang-Ming University, Taipei, Taiwan. FAU - Tsai, Hong-Chieh AU - Tsai HC AD - Department of Neurosurgery, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan; Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan; School of Traditional Chinese Medicine, Chang Gung University, Taoyuan 333, Taiwan. FAU - Chen, Shih-Pin AU - Chen SP AD - Department of Neurology, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan; Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan; Brain Research Center, National Yang-Ming University, Taipei, Taiwan. FAU - Lin, Anya Maan-Yuh AU - Lin AM AD - Department of Pharmacology, National Yang-Ming University, Taipei, Taiwan; Faculty of Pharmacy, National Yang-Ming University, Taipei, Taiwan; Department of Medical Research, Taipei-Veterans General Hospital, Taipei, Taiwan. FAU - Liu, Tsung-Yun AU - Liu TY AD - Institute of Food Safety and Health Risk Assessment, National Yang-Ming UniversRity, Taipei, Taiwan. FAU - Wang, Hsiang-Tsui AU - Wang HT AD - Department of Pharmacology, National Yang-Ming University, Taipei, Taiwan. Electronic address: htwang01@ym.edu.tw. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191017 PL - United States TA - Exp Neurol JT - Experimental neurology JID - 0370712 RN - 7864XYD3JJ (Acrolein) RN - EC 2.3.1.- (Acetyltransferases) RN - EC 2.3.1.57 (diamine N-acetyltransferase) RN - GAN16C9B8O (Glutathione) RN - U87FK77H25 (Spermidine) SB - IM MH - Acetyltransferases/*metabolism MH - Acrolein/*metabolism MH - Aged MH - Animals MH - Brain/metabolism MH - Enzyme Activation/physiology MH - Female MH - Glutathione/metabolism MH - Humans MH - Male MH - Middle Aged MH - Rats MH - Signal Transduction/*physiology MH - Spermidine/*metabolism MH - Stroke/*metabolism OTO - NOTNLM OT - 3-HPMA OT - Acrolein OT - Glutathione OT - Ischemic stroke OT - Neurotoxicity OT - SSAT EDAT- 2019/10/21 06:00 MHDA- 2020/07/22 06:00 CRDT- 2019/10/21 06:00 PHST- 2019/07/04 00:00 [received] PHST- 2019/09/06 00:00 [revised] PHST- 2019/09/18 00:00 [accepted] PHST- 2019/10/21 06:00 [pubmed] PHST- 2020/07/22 06:00 [medline] PHST- 2019/10/21 06:00 [entrez] AID - S0014-4886(19)30215-8 [pii] AID - 10.1016/j.expneurol.2019.113066 [doi] PST - ppublish SO - Exp Neurol. 2020 Jan;323:113066. doi: 10.1016/j.expneurol.2019.113066. Epub 2019 Oct 17.