PMID- 31630178 OWN - NLM STAT- MEDLINE DCOM- 20200930 LR - 20231203 IS - 1460-2377 (Electronic) IS - 0953-8178 (Print) IS - 0953-8178 (Linking) VI - 32 IP - 2 DP - 2020 Feb 7 TI - Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia. PG - 133-141 LID - 10.1093/intimm/dxz071 [doi] AB - Accumulating evidence has revealed that lymphoid tissue-resident commensal bacteria (e.g. Alcaligenes spp.) survive within dendritic cells. We extended our previous study by investigating microbes that persistently colonize colonic macrophages. 16S rRNA-based metagenome analysis using DNA purified from murine colonic macrophages revealed the presence of Stenotrophomonas maltophilia. The in situ intracellular colonization by S. maltophilia was recapitulated in vitro by using bone marrow-derived macrophages (BMDMs). Co-culture of BMDMs with clinically isolated S. maltophilia led to increased mitochondrial respiration and robust IL-10 production. We further identified a 25-kDa protein encoded by the gene assigned as smlt2713 (recently renamed as SMLT_RS12935) and secreted by S. maltophilia as the factor responsible for enhanced IL-10 production by BMDMs. IL-10 production is critical for maintenance of the symbiotic condition, because intracellular colonization by S. maltophilia was impaired in IL-10-deficient BMDMs, and smlt2713-deficient S. maltophilia failed to persistently colonize IL-10-competent BMDMs. These findings indicate a novel commensal network between colonic macrophages and S. maltophilia that is mediated by IL-10 and smlt2713. CI - (c) The Author(s) 2019. Published by Oxford University Press on behalf of The Japanese Society for Immunology. FAU - Takahashi, Ichiro AU - Takahashi I AD - Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. AD - Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan. FAU - Hosomi, Koji AU - Hosomi K AD - Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan. FAU - Nagatake, Takahiro AU - Nagatake T AD - Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan. FAU - Toubou, Hirokazu AU - Toubou H AD - Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. FAU - Yamamoto, Daiki AU - Yamamoto D AD - Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. FAU - Hayashi, Ikue AU - Hayashi I AD - Department of Mucosal Immunology, Graduate School of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan. FAU - Kurashima, Yosuke AU - Kurashima Y AD - International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. FAU - Sato, Shintaro AU - Sato S AD - International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. FAU - Shibata, Naoko AU - Shibata N AD - International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. FAU - Goto, Yoshiyuki AU - Goto Y AD - International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. FAU - Maruyama, Fumito AU - Maruyama F AD - Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan. FAU - Nakagawa, Ichiro AU - Nakagawa I AD - Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan. FAU - Kuwae, Asaomi AU - Kuwae A AD - Laboratory of Bacterial Infection, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan. FAU - Abe, Akio AU - Abe A AD - Laboratory of Bacterial Infection, Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan. FAU - Kunisawa, Jun AU - Kunisawa J AD - Laboratory of Vaccine Materials, Center for Vaccine and Adjuvant Research and Laboratory of Gut Environmental System, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-Osaka, Japan. AD - International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. AD - Graduate School of Medicine, Graduate School of Pharmaceutical Sciences, and Graduate School of Dentistry, Osaka University, Suita-Osaka, Japan. AD - Graduate School of Medicine, Kobe University, Kobe-Hyogo, Japan. FAU - Kiyono, Hiroshi AU - Kiyono H AD - International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Int Immunol JT - International immunology JID - 8916182 RN - 0 (IL10 protein, mouse) RN - 130068-27-8 (Interleukin-10) SB - IM MH - Animals MH - Coculture Techniques MH - Female MH - Homeostasis/immunology MH - Interleukin-10/deficiency/immunology MH - Macrophages/*immunology/microbiology MH - Mice MH - Mice, Inbred BALB C MH - Mice, Inbred C57BL MH - Mice, Knockout MH - Mice, SCID MH - Stenotrophomonas maltophilia/*immunology PMC - PMC10689348 OTO - NOTNLM OT - IL-10 OT - colitis OT - gut-resident macrophage OT - intracellular commensal bacteria OT - symbiotic factor smlt2713 EDAT- 2019/10/21 06:00 MHDA- 2020/10/02 06:00 PMCR- 2019/10/20 CRDT- 2019/10/21 06:00 PHST- 2019/09/24 00:00 [received] PHST- 2019/10/17 00:00 [accepted] PHST- 2019/10/21 06:00 [pubmed] PHST- 2020/10/02 06:00 [medline] PHST- 2019/10/21 06:00 [entrez] PHST- 2019/10/20 00:00 [pmc-release] AID - 5601250 [pii] AID - dxz071 [pii] AID - 10.1093/intimm/dxz071 [doi] PST - ppublish SO - Int Immunol. 2020 Feb 7;32(2):133-141. doi: 10.1093/intimm/dxz071.