PMID- 31630258
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20200909
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 186
IP  - 11
DP  - 2019 Oct 19
TI  - Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using 
      platinum nanoclusters as a label in an immunoassay.
PG  - 705
LID - 10.1007/s00604-019-3868-9 [doi]
AB  - The authors describe the use of platinum nanoclusters (PtNCs) as bimodal labels 
      in a competitive immunoassay for immunoglobulin E (IgE). Both fluorometry and 
      inductively coupled plasma - mass spectrometry (ICP-MS) are used. Optimization of 
      the PtNCs synthesis using lipoic acid as ligand was carried out. The time for 
      synthesis and the effect of NaOH added to the PtNCs precursor mixture was 
      optimized with the aim to obtain PtNCs with strong red fluorescence and low size 
      dispersity. Maximal fluorescence was obtained at excitation/emission wavelengths 
      of 455/620 nm. The average diameter (1.5 nm) and crystal structure (face-centered 
      cubic structure) of the PtNCs were determined by HR-TEM. It was calculated that 
      each PtNC contains 116 Pt atoms at average. Labelling of the antibody (Ab) 
      against IgE with PtNCs was optimized in terms of recognition capabilities and 
      fluorescence intensity. A molar ratio (Ab:PtNCs) of 1:11 is found to be best. A 
      competitive immunoassay for IgE was developed and detection was carried out by 
      using both ICP-MS (by measuring (195)Pt) and fluorometry. The limit of detection 
      (LOD) of the fluoroimmunoassay is 0.6 ng mL(-1) of IgE. The LOD of the ICP-MS 
      method is as low as 0.08 ng mL(-1). The method was evaluated by analyzing four 
      (spiked) serum samples by ICP-MS. No sample pretreatment excepting dilution is 
      needed. Results compared favorably with those obtained by a commercial ELISA kit. 
      Graphical abstract Schematic representation of the bimodal quantification 
      (fluorescence and ICP-MS) of immunoglobulin E (Ig E) in human serum using 
      antibody against human Ig E, labelled with several platinum nanoclusters (NCs) as 
      immunoprobe. Elemental mass spectrometry (MS) allows high amplification of the 
      signal because of the high number of platinum atoms per nanocluster (~116 Pt/NC).
FAU - Lores-Padin, Ana
AU  - Lores-Padin A
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain.
FAU - Cruz-Alonso, Maria
AU  - Cruz-Alonso M
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain.
FAU - Gonzalez-Iglesias, Hector
AU  - Gonzalez-Iglesias H
AD  - Instituto Universitario Fernandez-Vega (Fundacion de Investigacion Oftalmologica, 
      Universidad de Oviedo), Avda. Dres. Fernandez-Vega, 34, 33012, Oviedo, Spain. 
      h.gonzalez@fio.as.
AD  - Instituto Oftalmologico Fernandez-Vega, Avda. Dres. Fernandez-Vega, 34, 33012, 
      Oviedo, Spain. h.gonzalez@fio.as.
FAU - Fernandez, Beatriz
AU  - Fernandez B
AUID- ORCID: 0000-0002-2592-1442
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain. fernandezbeatriz@uniovi.es.
AD  - Instituto Universitario Fernandez-Vega (Fundacion de Investigacion Oftalmologica, 
      Universidad de Oviedo), Avda. Dres. Fernandez-Vega, 34, 33012, Oviedo, Spain. 
      fernandezbeatriz@uniovi.es.
FAU - Pereiro, Rosario
AU  - Pereiro R
AD  - Department of Physical and Analytical Chemistry, University of Oviedo, Julian 
      Claveria 8, 33006, Oviedo, Spain.
AD  - Instituto Universitario Fernandez-Vega (Fundacion de Investigacion Oftalmologica, 
      Universidad de Oviedo), Avda. Dres. Fernandez-Vega, 34, 33012, Oviedo, Spain.
LA  - eng
GR  - CTQ2016-79015-R/Agencia estatal de investigacion/International
GR  - MECD-17-FPU16/01363/Ministerio de Educacion/International
GR  - RYC-2014-1498/Ministerio Economia y Competitividad/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191019
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - 0 (Fluorescent Dyes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 49DFR088MY (Platinum)
SB  - IM
MH  - Fluorescent Dyes/*chemistry
MH  - Fluoroimmunoassay/methods
MH  - Fluorometry/methods
MH  - Humans
MH  - Immunoglobulin E/*blood/immunology
MH  - Limit of Detection
MH  - Mass Spectrometry/methods
MH  - Metal Nanoparticles/*chemistry
MH  - Platinum/chemistry
MH  - Proof of Concept Study
OTO - NOTNLM
OT  - Carbodiimide crosslinking
OT  - Elemental mass spectrometry
OT  - Fluoroimmunoassay
OT  - Immunoprobe
OT  - Lipoic acid
OT  - One-pot synthesis
EDAT- 2019/10/21 06:00
MHDA- 2020/04/14 06:00
CRDT- 2019/10/21 06:00
PHST- 2019/05/21 00:00 [received]
PHST- 2019/09/20 00:00 [accepted]
PHST- 2019/10/21 06:00 [entrez]
PHST- 2019/10/21 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
AID - 10.1007/s00604-019-3868-9 [pii]
AID - 10.1007/s00604-019-3868-9 [doi]
PST - epublish
SO  - Mikrochim Acta. 2019 Oct 19;186(11):705. doi: 10.1007/s00604-019-3868-9.