PMID- 31631599
OWN - NLM
STAT- MEDLINE
DCOM- 20191114
LR  - 20220411
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 50
IP  - 3
DP  - 2019 May
TI  - [Renal Injury and Its Mechanisms in Lepr (db/ db) Mice].
PG  - 334-338
AB  - OBJECTIVE: To study the mechanism of renal injury in Lepr (db/ db) mice with the 
      leptin receptor homozygous deficiency. METHODS: Ten male of 28-week-old Lepr 
      (db/+) mice with leptin receptor heterozygous deficiency were selected as control 
      group and ten male Lepr (db/ db) mice with leptin receptor homozygous deficiency 
      were used in this study. After fasting for 8 hours, the body mass, fasting blood 
      glucose (FBG) and glycosylated hemoglobulin (HbA1c) of the mice were measured. 
      Blood of the mice was obtained from femoral artery before euthanasia. Serum 
      creatinine (CRE), blood urea nitrogen (BUN), superoxide dismutase (SOD), 
      glutathione (GSH) and malonaldehyde (MDA) were detected by corresponding kits, 
      and serum interleukin-1beta (IL-1beta), monocyte chemotactic protein-1 (MCP-1) and 
      tumor necrosis factor-alpha (TNF-alpha) were measured using enzyme-linked immunosorbent 
      assay (ELISA) method. The kidney was taken for pathological observation. The 
      expression levels of nuclear factor E2-related factor 2 (Nrf2) and nuclear factor 
      kappa B (NF-kappaB) in renal were analyzed by Western blot. The mitochondria of renal 
      was isolated by the corresponding kit. Meanwhile, the expression level of lipoic 
      acid synthase (LIAS) in renal mitochondria was measured by Western blot. RESULTS: 
      The body mass, FPG, HbA1c, CRE and BUN levels of the Lepr (db/ db) mice were 
      significantly increased in comparison with the Lepr (db/+) mice ( P<0.05). 
      Compared with the Lepr (db/+) mice, the Lepr (db/ db) mice renal exhibited 
      glomerular hypertrophy, thickened basement membrane and capillary wall, the 
      mesangial matrix expansion and mesangial cell hyperplasia. Compared with the Lepr 
      (db/+) mice, the serum level of GSH in the Lepr (db/ db) mice was decreased 
      significantly ( P<0.05). The levels of MDA and concentrations of MCP-1, IL-1beta and 
      TNF-alpha in serum of the Lepr (db/ db) mice were higher than those of the Lepr 
      (db/+) mice ( P<0.05). Compared with the Lepr (db/+) mice, the expression of LIAS 
      and Nrf2 protein in the Lepr (db/ db) mice renal were decreased ( P<0.05), while 
      the expression of NF-kappaB protein was increased ( P<0.05). CONCLUSION: LIAS, Nrf2 
      and NF-kappaB might play significant roles through regulation of oxidative stress and 
      inflammation in the renal injury of Lepr (db/ db) mice.
CI  - Copyright(c) by Editorial Board of Journal of Sichuan University (Medical Science 
      Edition).
FAU - Yan, Ting-Ting
AU  - Yan TT
AD  - Department of Toxicology, School of Public Health, Xinxiang Medical University, 
      Xinxiang 453003, China.
FAU - Xu, Guang-Cui
AU  - Xu GC
AD  - Department of Toxicology, School of Public Health, Xinxiang Medical University, 
      Xinxiang 453003, China.
FAU - Peng, Qiang
AU  - Peng Q
AD  - Department of Toxicology, School of Public Health, Xinxiang Medical University, 
      Xinxiang 453003, China.
FAU - Wu, Wei-Dong
AU  - Wu WD
AD  - Department of Toxicology, School of Public Health, Xinxiang Medical University, 
      Xinxiang 453003, China.
FAU - Yi, Xian-Wen
AU  - Yi XW
AD  - Department of Toxicology, School of Public Health, Xinxiang Medical University, 
      Xinxiang 453003, China.
FAU - Zhao, Ying-Zheng
AU  - Zhao YZ
AD  - Department of Toxicology, School of Public Health, Xinxiang Medical University, 
      Xinxiang 453003, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Receptors, Leptin)
RN  - EC 2.8.1.- (Sulfurtransferases)
RN  - EC 2.8.1.- (lipoic acid synthase)
SB  - IM
MH  - Animals
MH  - Kidney/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - NF-E2-Related Factor 2/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Oxidative Stress
MH  - Receptors, Leptin/*genetics
MH  - Sulfurtransferases/*metabolism
OTO - NOTNLM
OT  - LIAS
OT  - Leprdb/db mice
OT  - NF-kappaB
OT  - Nrf2
OT  - Renal injury
EDAT- 2019/10/22 06:00
MHDA- 2019/11/15 06:00
CRDT- 2019/10/22 06:00
PHST- 2019/10/22 06:00 [entrez]
PHST- 2019/10/22 06:00 [pubmed]
PHST- 2019/11/15 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2019 May;50(3):334-338.